The qpcR Package - NexTag Supports Open Source Initiatives

More documents

Recommendations

Info

18 pcrbatchAuthor(s)Andrej-Nikolai SpiessExamplesm
pcrfit 19DetailsValue<strong>The</strong> qPCR raw data should be arranged with the cycle numbers in the first column with the name"Cycles". All subsequent columns must be plain raw data with sensible column descriptions. Ifreplicates are defined, the output will contain a numbering of groups (i.e. "group1" for the firstreplicate group). <strong>The</strong> model selection process is optional, but we advocate using this for obtainingbetter parameter estimates. Normalization has been described to improve certain qPCR analyses,but this has still to be independently evaluated.A dataframe with the results in columns containing the calculated values with descriptions and themethod used as the name prefix.Author(s)Andrej-Nikolai SpiessReferencesA standard curve based method for relative real time PCR data processing. Larionov et al., BMCBioinformatics, 6: 62.Examples## complete dataset## Not run:temp
Page 1 and 2: The qpcR PackageFebruary 16, 2008Ty
Page 3 and 4: RMSE 3Author(s)Andrej-Nikolai Spies
Page 5 and 6: calib 5DetailsThe real-time PCR was
Page 7 and 8: confband 7Examples###define dilutio
Page 9 and 10: efficiency 9Examplesm
Page 11 and 12: evidence 11efficiency(m2, type = "m
Page 13 and 14: expfit 13expfitCalculation of PCR e
Page 15 and 16: mchoice 15ValueA list with the foll
Page 17: pcrGOF 17DetailsValueFor a more det
Page 21 and 22: pcropt1 21Argumentsfilesepheaderquo
Page 23 and 24: pcrplot 23pcrplotPlotting PCR data
Page 25 and 26: qpcR 25Usagepcrpred(object, newdata
Page 27 and 28: epform 27confband qPCR data from Ru
Page 29 and 30: eps2 29Examplesdata(reps)### on sin
Page 31 and 32: esVar 31resVarResidual variance of
Page 33 and 34: sliwin 33DetailsValueTo avoid fits
Page 35: INDEX 35mchoice, 15modlist, 16multd

The qpcR Package - NexTag Supports Open Source Initiatives

Create successful ePaper yourself

Delete template?

Save as template?