The qpcR Package - NexTag Supports Open Source Initiatives

30 reps3reps3 A qPCR dilution experiment with replicates (Stratagene MX-Pro3000P)DescriptionA high quality dilution experiment with six 4-fold dilutions of the cDNA, and three replicates foreach dilution.Usagedata(reps3)FormatA data frame with the PCR cycles and 21 qPCR runs with three replicates of six 4-fold dilutions.The replicates are defined by FX.Y (X = dilution number, Y = replicate number).DetailsThe real-time PCR was conducted with primers for the S27a housekeeping gene in a MXPro3000Pinstrument (Stratagene). Data was ROX-normalized, but without smoothing.SourceHeike Cappallo-Obermann, Bone Marrow Transplantation Unit, University Hospital Hamburg-Eppendorf.Examplesdata(reps3)### on single datam1