The qpcR Package - NexTag Supports Open Source Initiatives

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32 sliwinDetailsThe real-time PCR was conducted for a 102 bp amplicon in an Opticon2 instrument.SourceSupplemental data 1 to the paper.ReferencesSigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developingautomated high-throughput applications. Rutledge RG, Nucleic Acids Research, 2004, 32, e178.Examplesdata(rutledge)### on single datam1
sliwin 33DetailsValueTo avoid fits with a high R-squared in the baseline region, the second derivative maximum is takenas a fixpoint. This value is consequently always near to the exponential region of the data and avoidsthe problem above. The efficiency is calculated by E = exp(slope), as the transformed raw datawas based on the natural logarithm. The initial template fluorescence (F0) is thus calculated byF 0 = exp(intercept).A list with the following components:effmaxrmaxinitthe maximal PCR efficiency.the maximum regression coefficient.the initial template fluorescence, i.e. ’F0’.Author(s)Andrej-Nikolai SpiessReferencesAssumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. RamakersC et al., Neuroscience Letters, 2003, 339, 62-66.Examplesm
Page 1 and 2: The qpcR PackageFebruary 16, 2008Ty
Page 3 and 4: RMSE 3Author(s)Andrej-Nikolai Spies
Page 5 and 6: calib 5DetailsThe real-time PCR was
Page 7 and 8: confband 7Examples###define dilutio
Page 9 and 10: efficiency 9Examplesm
Page 11 and 12: evidence 11efficiency(m2, type = "m
Page 13 and 14: expfit 13expfitCalculation of PCR e
Page 15 and 16: mchoice 15ValueA list with the foll
Page 17 and 18: pcrGOF 17DetailsValueFor a more det
Page 19 and 20: pcrfit 19DetailsValueThe qPCR raw d
Page 21 and 22: pcropt1 21Argumentsfilesepheaderquo
Page 23 and 24: pcrplot 23pcrplotPlotting PCR data
Page 25 and 26: qpcR 25Usagepcrpred(object, newdata
Page 27 and 28: epform 27confband qPCR data from Ru
Page 29 and 30: eps2 29Examplesdata(reps)### on sin
Page 31: esVar 31resVarResidual variance of
Page 35: INDEX 35mchoice, 15modlist, 16multd

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