Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

HUMAN MOLECULAR DIAGNOSTICS CATALOGUE 2011GASTROENTERITIS INFECTIONSNorovirus I & IIRef: Dia-Norovirus (I/II)-050Norovirus is an RNA virus that causes approximately 90% ofepidemic non-bacterial outbreaks of gastroenteritis around theworld (1) , and may be responsible for 50% of all foodborne outbreaksof gastroenteritis in the US (2) . Norovirus affects people of all ages.The viruses are transmitted by faecally contaminated food orwater, by person-to-person contact (3) and via aerosolization of thevirus and subsequent contamination of surfaces (4) .(1)Lindesmith L, Moe C, Marionneau S, et al. (2003). «Human susceptibility andresistance to Norwalk virus infection». Nat. Med. 9 (5): 548–53. (2) WiddowsonMA, Sulka A, Bulens SN, et al. (2005). «Norovirus and foodborne disease,United States, 1991-2000». Emerging Infect. Dis. 11 (1): 95–102. (3) GoodgameR (2006). «Norovirus gastroenteritis». Curr Gastroenterol Rep 8 (5): 401–8.(4)Said MA, Perl TM, Sears CL (November 2008). «Healthcare epidemiology:gastrointestinal flu: norovirus in health care and long-term care facilities».Clinical Infectious Diseases : an Official Publication of the Infectious DiseasesSociety of America 47 (9): 1202–8.VALIDATIONResearched pathogens:• Norovirus 1: FAM dye, emission: 520 nm• Norovirus 2: yellow dye, emission: 553 nmTarget genes: • RdRp/Capsid junction (Norovirus 1)• RdRp/Capsid junction) (Norovirus 2)Number of wells by test: 1 (Norovirus 1 / Norovirus 2)Samples used:• StoolValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• QIAamp DNA Stool mini kit (Qiagen)• MagNa pure LC system (Roche)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche lightCycler (480)• Biorad (ICycler/IQ5/CFX96)• Qiagen RotorGene (6000)• Stratagene (MX3000P/3005P)• Cepheid (SmartCycler II)PCR volumes: 50 µl, 25 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 (50 µl PCR)• 100 (25 µl PCR)In combination with:• Universal inhibition control (DIA-UIC-050):DIA-UIC(DR)-050: orange dye, emission: 576 nmDIA-UIC(TR)-050: texas red dye, emission: 603 nmDIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm• RNA extraction & PCR inhibition control (DIA-EIC/RNA)-050:DIA-EIC/RNA(DR)-050: orange dye, emission: 576 nmDIA-EIC/RNA(TR)-050: texas red dye, emission: 603 nmDIA-EIC/RNA(Cy5)-050: Cy5 dye, emission: 662 nm14 | www.diagenodediagnostics.com