Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

Human respiratory syncytialvirusRef: Dia-RSV-050Human respiratory syncytial virus (RSV) is a respiratory infectionof upper and lower respiratory tract in infants and young children.It causes bronchiolitis and pneumonia.Human respiratory syncytial virus is a negative-sense, envelopedRNA virus. The virion is variable in shape and size (averagediameter from 120 to 300 nm). It is also unstable in the environment(surviving only a few hours on environmental surfaces).VALIDATIONResearched pathogen:• Human respiratory syncytial virus: FAM dye, emission: 520 nmTarget genes:• N (Human respiratory syncytial virus)Number of wells by test: 1 (Human respiratory syncytial virus)Samples used:• Bronchoalveolar lavage• Sputum• Nasopharyngeal swabs/washesValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• QIAamp ® Viral RNA Mini Kit (Qiagen)• MagNa pure LC system (Roche)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche LightCycler ® (2.0/480)• Biorad (iCycler/IQ5/CFX96)• Qiagen Rotor-Gene (6000)• Stratagene (MX3000P/3005P)• Cepheid (SmartCycler II)PCR volumes: 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 reactions (50 µl PCR, final volume)• 100 reactions (25 µl PCR, final volume)• 120 reactions (20 µl PCR, final volume)In combination with:• Universal inhibition control (DIA-UIC-050):DIA-UIC(YD)-050: yellow dye, emission: 549 nmDIA-UIC(DR)-050: orange dye, emission: 576 nmDIA-UIC(TR)-050: texas red dye, emission: 603 nmDIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm• RNA extraction & PCR inhibition control (DIA-EIC/RNA)-050:DIA-EIC/RNA(YD)-050: yellow dye, emission: 549 nmDIA-EIC/RNA(DR)-050: orange dye, emission: 576 nmDIA-EIC/RNA(TR)-050: texas red dye, emission: 603 nmDIA-EIC/RNA(Cy5)-050: Cy5 dye, emission: 662 nm31