Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

HUMAN MOLECULAR DIAGNOSTICS CATALOGUE 2011RESPIRATORY INFECTIONSMycobacterium tuberculosisRef: Dia-MT-050Mycobacterium tuberculosis (MTB) is a pathogenic bacterialspecies of the genus Mycobacterium and the causative agent ofmost cases of tuberculosis.Tuberculosis is a common and severe infectious disease thatusually attacks the lungs but can also affect the central nervoussystem, the lymphatic system, the circulatory system, thegenitourinary system, the gastrointestinal system, bones, joints,and even the skin.Other mycobacteria such as Mycobacterium bovis, Mycobacteriumafricanum, Mycobacterium canetti, and Mycobacterium microtialso cause tuberculosis, but these species are less common.VALIDATIONResearched pathogen:• Mycobacterium tuberculosis: FAM dye, emission: 520 nmTarget genes:• IS6110 (Mycobacterium tuberculosis)Number of wells by test: 1 (Mycobacterium tuberculosis)Samples used:• Bronchoalveolar lavage• SputumValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• QIAamp ® DNA Mini Kit (50) (Blood and Body Fluid Spin Protocol) (Qiagen)• MagNa pure LC system (Roche)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche LightCycler ® (2.0/480)• Biorad (iCycler/IQ5/CFX96)• Qiagen Rotor-Gene (6000)• Stratagene (MX3000P/3005P)• Cepheid (SmartCycler II)PCR volumes: 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 reactions (50 µl PCR, final volume)• 100 reactions (25 µl PCR, final volume)• 120 reactions (20 µl PCR, final volume)In combination with:• Universal inhibition control (DIA-UIC-050):DIA-UIC(YD)-050: yellow dye, emission: 549 nmDIA-UIC(DR)-050: orange dye, emission: 576 nmDIA-UIC(TR)-050: texas red dye, emission: 603 nmDIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm• DNA extraction & PCR inhibition control (DIA-EIC/DNA)-050:DIA-EIC/DNA(YD)-050: yellow dye, emission: 549 nmDIA-EIC/DNA(DR)-050: orange dye, emission: 576 nmDIA-EIC/DNA(TR)-050: texas red dye, emission: 603 nmDIA-EIC/DNA(Cy5)-050: Cy5 dye, emission: 662 nm34 | www.diagenodediagnostics.com