Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

Diagenode’s wide range of kits enables new levels of throughput, cost savingsand ease-of-use for real-time qPCR IVD.HIGHER THROUGHPUT LOWER COSTALL EXTRACTION METHODS ALL qPCR PLATFORMSALL MASTER MIXEST0T130’ 60’QC in the Extraction Step*Patient sampleDiagenode’s extractionand inhibition controlSmart qPCR Set-UpOur kits are designed and validated for a variety ofreal-time qPCR platforms** as well as for differentextraction protocols. The kits are validated with themost commonly-used master mixes. To achieve optimalsensitivity with our kits, we provide recommendations onwhich master mixes will provide the best results.KIT 3Ex: LegionellaKIT 1Ex: Varicella zoster virusKIT 2Ex: CytomegalovirusOnly one set of qPCR cycles for ALL Diagenode’s kits allows costand time savings. The same PCR plate can be used for testingdifferent pathogens.SampleBPositive controlDiagenode suggests using DNA or RNA extraction andinhibition controls (Dia-EIC/DNA-050, Dia-EIC/RNA-050)for highest test accuracy. These controls are availablewith different dyes: YD, Cy5, DR and TR (see page 31).Note: If you do not use an extraction control, you can useour universal inhibition control (Dia-UIC-050). This contolis available with different dyes: YD, Cy5, DR and TR.Human sampleDiagenode’s primers andprobes (pathogen + ctl***)Master mix(Your favorite**)Diagenode’s positivecontrolDiagenode’s primers andprobes (pathogen + ctl***)Master mix(Your favorite**)* Most standard extraction methods are compatible withDiagenode’s kits.** Most have been validated with our kits. Ask for results!*** ctl = Dia-EIC/DNA-050, Dia-EIC/RNA-050 or Dia-UIC-050