Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

HUMAN MOLECULAR DIAGNOSTICS CATALOGUE 2011HERPES VIRUSESVaricella zoster virusRef: Dia-VZV-050Varicella zoster virus (VZV) is one of eight herpes viruses known toinfect humans. It commonly causes chicken-pox in children andboth shingles and postherpetic neuralgia in adults.Primary VZV infection results in chickenpox (varicella), which mayrarely result in complications including encephalitis or pneumonia.Even when clinical symptoms of chickenpox have resolved, VZVremains dormant in the nervous system of the infected person(virus latency), in the trigeminal and dorsal root ganglia (Steineret al., 2007). In about 10-20% of cases, VZV reactivates later in lifeproducing a disease known as herpes zoster or shingles. Seriouscomplications of shingles include postherpetic neuralgia, zostermultiplex, myelitis, herpes ophthalmicus, or zoster sine herpete.Steiner I, Kennedy PG, Pachner AR (2007). «The neurotropic herpes viruses:herpes simplex and varicella-zoster». Lancet Neurol 6 (11): 1015–28.VALIDATIONResearched pathogen:• Varicella zoster virus: FAM dye, emission: 520 nmTarget genes:• Glycoprotein B (ORF 31 gene, Varicella zoster virus)Number of wells by test: 1 (Varicella zoster virus)Samples used:• Bronchoalveolar lavage• Tissue/biopsy• Vesicular fluid• Ocular fluid• Swab corneal scraping• Cerebrospinal fluid• Amniotic fluid• Plasma• SerumValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• MagNa pure LC system (Roche)• QIAamp ® UltraSens Virus Kit (Qiagen)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche LightCycler ® (2.0/480)• Biorad (iCycler/IQ5/CFX96)• Qiagen Rotor-Gene (6000)• Stratagene (MX3000P/3005P)• Cepheid (SmartCycler II)PCR volumes: 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 reactions (50 µl PCR, final volume)• 100 reactions (25 µl PCR, final volume)• 120 reactions (20 µl PCR, final volume)In combination with:• Universal inhibition control (DIA-UIC-050):DIA-UIC(YD)-050: yellow dye, emission: 549 nmDIA-UIC(DR)-050: orange dye, emission: 576 nmDIA-UIC(TR)-050: texas red dye, emission: 603 nmDIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm• DNA extraction & PCR inhibition control (DIA-EIC/DNA)-050:DIA-EIC/DNA(YD)-050: yellow dye, emission: 549 nmDIA-EIC/DNA(DR)-050: orange dye, emission: 576 nmDIA-EIC/DNA(TR)-050: texas red dye, emission: 603 nmDIA-EIC/DNA(Cy5)-050: Cy5 dye, emission: 662 nm24 | www.diagenodediagnostics.com