Human Infectiology MDx | Catalogue 2011 - Diagenode Diagnostics

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HUMAN MOLECULAR DIAGNOSTICS CATALOGUE 2011RESPIRATORY INFECTIONSRespiratory RNA virusesmultiplexRef: Dia-ResRNAVir-050Human influenza (HI) A/B, human respiratory syncytial virus(RSV), human metapneumovirus (MPV), human rhinovirus (HRV)and human parainfluenza (HPIV) 1/2/3/4 are respiratory infectionsof upper and lower respiratory tract in infants and young children.They cause bronchiolitis and pneumonia.Influenza is a negative-sense, enveloped RNA virus. Its morphologyis spherical or filamentous with a diameter of 80 to120 nm. Itsmost severe symptoms are associated with influenza A viruses.Many sub-types of influenza A viruses are known and classifiedaccording to the origin of the hemagglutinin and neuraminidase.Influenza B viruses are antigenically more stable than influenza A.This explains that they cause less severe epidemics.RSV is a negative-sense, enveloped RNA virus. The virion isvariable in shape and size (average diameter from 120 to 300 nm).It is also unstable in the environment (surviving only a few hourson environmental surfaces).MPV is a negative-sense, single stranded RNA virus. Earlyassessments suggest that this virus may be similar to respiratorysyncytial virus in having two distinct genotypes and a similarepidemiology.HRV is a positive-sense, single strand RNA virus within anicosahedral (20-sided) capsid. Approximately 101 serotypes arecurrently identified.HPIVs are a group of four different serotypes of single-strandedRNA viruses belonging to the paramyxovirus family. They are thesecond most common cause of lower respiratory tract infectionin younger children.36 | www.diagenodediagnostics.comVALIDATIONResearched pathogens:• Human influenza A: FAM dye, emission: 520 nm• Human influenza B: yellow dye, emission: 549 nm• Human respiratory syncytial virus: FAM dye, emission: 520 nm• Human metapneuvirus: FAM dye, emission: 520 nm• Human rhinovirus: yellow dye, emission: 549 nm• Human parainfluenza 1: FAM dye, emission: 520 nm• Human parainfluenza 2: FAM dye, emission: 520 nm• Human parainfluenza 3: yellow dye, emission: 549 nm• Human parainfluenza 4: yellow dye, emission: 549 nm(RNA extraction & PCR inhibition control: yellow dye, emission: 549 nm)Target genes: • MP (Human influenza A)• NP (Human influenza B)• N (Human respiratory syncytial virus)• N (Human metapneumovirus)• HN (Human parainfluenza 1)• HN (Human parainfluenza 2)• HN (Human parainfluenza 3)• Phosphoprotein (Human parainfluenza 4)Number of wells by test: 51. Human influenza A/ Human influenza B2. Human respiratory syncytial virus/ RNA extraction & PCR inhibition control3. Human metapneuvirus/ Human rhinovirus4. Human parainfluenza 1/ Human parainfluenza 35. Human parainfluenza 2/ Human parainfluenza 4Samples used:• Bronchoalveolar lavage• Sputum• Nasopharyngeal swabs/washesValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• QIAamp ® Viral RNA Mini Kit (Qiagen)• MagNa pure LC system (Roche)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche LightCycler ® (480/2.0 on request)• Biorad (iCycler/IQ5/CFX96)• Qiagen Rotor-Gene (6000)• Stratagene (MX3000P/3005P)PCR volumes: 50 µl, 25 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 reactions (50 µl PCR, final volume)• 100 reactions (25 µl PCR, final volume)
Swine influenzaRef: Dia-swH1N1-050Swine influenza (also called H1N1 flu, swine flu) is an infection byany one of several types of swine influenza virus. Swine influenzavirus (SIV) is any strain of the influenza family of viruses thatis endemic in pigs. As of 2009, the known SIV strains includeinfluenza C and the subtypes of influenza A known as H1N1,H1N2, H3N1, H3N2, and H2N3 (1) .Transmission of the virus from pigs to humans is not commonand does not always lead to human influenza, often resulting onlyin the production of antibodies in the blood. If transmission doescause human influenza, it is called zoonotic swine flu.Swine influenza. The Merck Veterinary Manual. 2008. Retrieved on April 30, 2009.http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/121407.htm.VALIDATIONResearched pathogen:• Swine H1N1 influenza A virus: FAM dye, emission: 520 nmTarget genes:• HA (Swine H1N1 influenza A virus)Number of wells by test: 1 (Swine H1N1 influenza A virus)Samples used:• Bronchoalveolar lavage• Nasopharyngeal swabs/washesValidated extraction methods: • Nuclisens easyMAG ® System (Biomerieux)• MagNa pure LC system (Roche)• QIAamp ® Viral RNA Mini Kit (Qiagen)Real-time PCR systems: • ABI (7000/7300/7500/7900)• Roche LightCycler ® (2.0/480)• Biorad (iCycler/IQ5/CFX96)• Qiagen Rotor-Gene (6000)• Stratagene (MX3000P/3005P)• Cepheid (SmartCycler II)PCR volumes: 50 µl, 25 µl, 20 µl (depending of the real-time PCR system used)PCR reactions/kit:• 50 reactions (50 µl PCR, final volume)• 100 reactions (25 µl PCR, final volume)• 120 reactions (20 µl PCR, final volume)In combination with:• Universal inhibition control (DIA-UIC-050):DIA-UIC(YD)-050: yellow dye, emission: 549 nmDIA-UIC(DR)-050: orange dye, emission: 576 nmDIA-UIC(TR)-050: texas red dye, emission: 603 nmDIA-UIC(Cy5)-050: Cy5 dye, emission: 662 nm• RNA extraction & PCR inhibition control (DIA-EIC/RNA)-050:DIA-EIC/RNA(YD)-050: yellow dye, emission: 549 nmDIA-EIC/RNA(DR)-050: orange dye, emission: 576 nmDIA-EIC/RNA(TR)-050: texas red dye, emission: 603 nmDIA-EIC/RNA(Cy5)-050: Cy5 dye, emission: 662 nm37
Page 3 and 4: The secure way to performanceCATALO
Page 5 and 6: The Technology of Diagenode’s IVD
Page 7 and 8: Universal inhibition controlRef: Di
Page 9 and 10: Gastroenteritisbacteria multiplexRe
Page 11 and 12: Dientamoeba fragilisRef: Dia-DF-050
Page 13 and 14: Gastroenteritisviruses multiplexRef
Page 15 and 16: Chlamydia trachomatisRef: Dia-CT-05
Page 17 and 18: Mycoplasma genatiliumRef: Dia-MG-05
Page 19: Trichomonas vaginalisRef: Dia-TV-05
Page 22 and 23: HUMAN MOLECULAR DIAGNOSTICS CATALOG
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Page 47 and 48: Diagenode’s expertise the secure

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