2011-2012 Catalogue - PeproTech, Inc.

www.peprotech.com2011-2012 CatalogueAntigen Affinity Purification of AntibodiesINFORMATION & TECHNICAL SUPPORTCytokines are signaling proteins necessary for cell- body production. Consequently, the sera obtained fromto-cell communication throughout the body. In accordancewith their role in preserving vital biological func-I-4) typically contain minute amounts of cytokine-spec-host animals after immunization with cytokines (Figuretions, the amino-acid sequence of homologous cytokines ific antibodies. Isolation of these antibodies by stanfromdifferent mammalian species is generally highly dard purification procedures (e.g. ion-exchange chromatography)is tedious and ineffective. On the other conserved, rendering them poor immunogens for anti-hand,Figure I-1 - Antigen Affinity Purification of Antibodies(A.) Preparation of separation resin. (B.) Specific binding of Antibody to the resin. (C.) pH elution of the antibody from the affinity column.154To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz