2011-2012 Catalogue - PeproTech, Inc.

www.peprotech.com2011-2012 CatalogueImmunohistochemistryINFORMATION & TECHNICAL SUPPORTThe analysis of tissues and cells for the presenceWorking with cells and tissue can pose difficultproblems due to the elaborate and complex natureof specific proteins is an important component of numerousclinical diagnostic and research programs. of these structures. In order to accurately examine theseImmunohistochemistry (histo=tissue) and immunocytochemistry(cyto=cell) are powerful techniques that uti- possible, the native state of the tissue being analyzed.complex samples it is vital to preserve, to the extentlize the specific interaction between a target protein and This is accomplished through a technique known asits corresponding antibody to visualize a protein of in- fixation. The fixation process is a cornerstone in immunohistochemistryand helps maintain the integrityterest in a complex biological sample. The terms immunohistochemistry(IHC) and immunocytochemistry and authenticity of the proteins and structures presentare often used interchangeably due to the intimate rela- in the samples. This is usually achieved by inhibitingtionship between cells and tissues. Two common de- the innate degradative processes present in the samplet e c t i o n m e t h o d s u s e d i n I H C a r e d i r e c t tissue by some combination of cryostasis, treatment withimmunoflouresence, in which the detecting antibody is chemical fixatives, and embedding tissue samples indirectly labeled with a fluorophore such as fluorescein paraffin. One of the most commonly used fixative comorrhodamine, and immunoenzymatic staining, in which binations is the formalin-fixed paraffin-embeddedthe detecting antibody is conjugated to a chromogen (FFPE) fixation method. To achieve maximum recognitionof the target protein, it is necessary to optimizeactivating enzyme either directly, or indirectly throughthe use of a conjugated secondary antibody.the fixation process for each sample type analyzed. Fac-Figure V-1 - Indirect Immunoenzymatic Detection Method172To Order: EC: Tel: +44 (0)20 7610 3062 • email: info@peprotech.co.ukBestellungen: AUSTRIA: Tel: +43 (0)1 405 9696 • email: info@peprotech.atObjednávky: Česká Rep.: Tel: +420 225 992 284 • email: info@peprotech.cz