Database on medicinal plants used in ayurveda - AYUSH Research ...

Komatsu et al., (1999) reported 103 types of proteins by a procedure for
separation and characterization of soluble proteins from suspension cultures
of rice.
Okamoto et al., (1996), reported the effect of oxygen percentage on
regeneration of plantlets from cell culture of rice. They maintained the callus
in bioreactors containing different media and a special attachment for supply
of oxygen. The observation showed that regeneration efficiency was
maximum in cultures provided with 40% dissolved oxygen available in or
provided with aeration 12 mg/L D.O. in controlled condition. Observations
were recorded that plants grown in bioreactor were 90% healthy.
Similarly, effect of CO2 on growth and survival of rice regenerants was
reported by Seko and Nishimura, (1996). They grew the rice regenerants on
sugar free medium under different concentration of CO2 (0.4, 50 and 100
mmol mol -1 ). Concentration of CO2 increased upto 50 mmol mol -1 was
found to be effective for survival and shoot growth of rice regenerants. CO2
at a concentration of 100 mmol mol l- and above caused decrease in survival
and over all growth of plantlets.
Seraj and coworkers (1997), conducted experiments to observe the response
of regeneration of callus derived from mature and immature embryos of 15
Indian rice varieties. They used modified MS and N6 medium for induction
of calli and regeneration, respectively. They also noted that some varieties
show regeneration response within 5-7 / 15-20 days. They also stated that
regeneration response of individual Indica rice varieties were unpredictable
because of large difference in regeneration percent, i.e. 0 to 97%.
Similarly, wide range of variation in morphological characteristics were
recorded by Ogura et al., 1987; Kanda et al., 1988; Lee et al., 1989; Su et al.,
1992 and Mezencev et al., 1995. Somaclonal variation was also reported by
Kharabian and Darabi, (2005). They observed cytological mutation in various
chromosomes in regenerated plants of rice.
REFERENCES
Akatsukal T; Kodama O; Koto H; Kono Y; Takeuchi S (1983), 3-Hydroxy-7-oxosandaracopimaradiene
(Oryzalexin-A), a new phytolexin isolated from rice blast leaves. Agri
Biol Chem. 47: 445.
Alemanno L; Guiderdoni E (1994), Increased doubled haploid plant regeneration from rice
(Oryza sativa L.) anthers cultured on colchicine-supplemented media. Plant Cell Reports.
13: 432-436.
Alvarez AM et al. (1995), Four rice seed cDNA clones belonging to the alphaamylase/trypsin
inhibitor gene family encode potential rice allergens, Biosci. Biotechnol. and
Biochem. 59(7) : 1304-1308.
337