15.12.2012 Views

When citing an abstract from the 2009 Annual - Society for ...

When citing an abstract from the 2009 Annual - Society for ...

When citing an abstract from the 2009 Annual - Society for ...

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 555.4/AA32<br />

Topic: D.01.d. Olfaction: Central circuits <strong>an</strong>d neurotr<strong>an</strong>smitters<br />

Support: NSERC<br />

OGS<br />

University of Ottawa<br />

Title: Elucidating <strong>the</strong> neural circuitry involved with sex pheromones signaling in male goldfish<br />

Authors: *W. E. LADO 1 , D. C. SPANSWICK 2 , J. E. LEWIS 1 , V. L. TRUDEAU 1 ;<br />

1 2<br />

Biol., Univ. of Ottawa, Ottawa, ON, C<strong>an</strong>ada; Warwick Med. Sch., Univ. of Warwick,<br />

COVENTRY, United Kingdom<br />

Abstract: The sex pheromones, 17 αλπηα, 20 βετα-dihydroxy-4-pregene-3-one <strong>an</strong>d<br />

prostagl<strong>an</strong>din-F2αλπηα, elicit specific reproductive behaviours in male goldfish. To better<br />

underst<strong>an</strong>d <strong>the</strong> neural circuitry involved with sex pheromones signalling in goldfish<br />

reproduction, we used male goldfish brain expl<strong>an</strong>ts. The adv<strong>an</strong>tage of working with <strong>the</strong> expl<strong>an</strong>ts<br />

is that <strong>the</strong> neural circuitry <strong>from</strong> <strong>the</strong> olfactory bulb (OB) to <strong>the</strong> preoptic area (POA) is preserved.<br />

We electrically stimulated <strong>the</strong> OB <strong>an</strong>d per<strong>for</strong>med whole-cell current clamp recordings <strong>from</strong><br />

neurons in <strong>the</strong> POA to discern <strong>the</strong> functional properties <strong>an</strong>d connections of this circuitry. These<br />

neurons fired action potentials spont<strong>an</strong>eously with some also displaying burst firing. We<br />

demonstrate evidence <strong>for</strong> a monosynaptic connection between <strong>the</strong> OB <strong>an</strong>d POA implying a role<br />

<strong>for</strong> sex pheromones signal tr<strong>an</strong>smission through <strong>the</strong> medial olfactory tract. A major component<br />

of <strong>the</strong> synaptic connection was blocked by DL-2-amino-5-phosphonopent<strong>an</strong>oic acid (AP5), <strong>an</strong><br />

N-Methyl-D-Aspartate (NMDA) receptor <strong>an</strong>tagonist. 6-cy<strong>an</strong>o-7-nitroquinoxaline-2, 3-dione<br />

(CNQX), a non NMDA receptor <strong>an</strong>tagonist, subsequently blocked <strong>the</strong> rest of <strong>the</strong> response.<br />

Overall, <strong>the</strong>se results indicate that <strong>the</strong> functional connection between <strong>the</strong> OB <strong>an</strong>d POA of male<br />

goldfish mediated by glutamatergic neurotr<strong>an</strong>smission is monosynaptic. This is consistent with<br />

<strong>the</strong> major role glutamate plays in <strong>the</strong> hormonal regulation of reproduction in male goldfish.<br />

Disclosures: W.E. Lado, None; D.C. Sp<strong>an</strong>swick, None; J.E. Lewis, None; V.L. Trudeau,<br />

None.<br />

Poster<br />

555. Olfactory Bulb: Synapses <strong>an</strong>d Circuits<br />

Location: South Hall A

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!