15.12.2012 Views

When citing an abstract from the 2009 Annual - Society for ...

When citing an abstract from the 2009 Annual - Society for ...

When citing an abstract from the 2009 Annual - Society for ...

SHOW MORE
SHOW LESS

You also want an ePaper? Increase the reach of your titles

YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.

Poster<br />

506. Neurogenesis II<br />

Location: South Hall A<br />

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 506.17/A38<br />

Topic: A.02.d. Neuronal differentiation<br />

Title: Analysis of mice with disruption of gamma protocadherin genes in excitatory neurons<br />

Authors: *Y. ITOGA 1 , S. HAMADA 2 , T. HIRABAYASHI 1 , T. YAGI 1 ;<br />

1 Kokoro Biol. Group, Grad. Sch. of Frontier Biosci., OSAKA UNIVERSITY, Suita / Osaka,<br />

Jap<strong>an</strong>; 2 Dev. Nutr. <strong>an</strong>d Hlth. Sci., Fukuoka Women's Univ., Fukuoka, Jap<strong>an</strong><br />

Abstract: Clustered protocadherins (Pcdhs) are diverse cell adhesion molecules. Genes encoding<br />

about 60 clustered Pcdhs are arr<strong>an</strong>ged in three clusters (Pcdhα, Pcdhβ, <strong>an</strong>d Pcdhγ) at a single<br />

chromosomal allele in mammali<strong>an</strong> genome. Among <strong>the</strong>m, Pcdhα <strong>an</strong>d Pcdhγ have multiple<br />

variable exons <strong>an</strong>d three const<strong>an</strong>t exons. Single exon of <strong>the</strong> multiple variable exons c<strong>an</strong> combine<br />

with three const<strong>an</strong>t exons by cis splicing of <strong>the</strong> mRNA. In <strong>the</strong> clustered Pcdh family, totally <strong>the</strong>re<br />

are about 60 distinct iso<strong>for</strong>ms. Each neuron expresses distinct combination of two or three each<br />

clustered Pcdh iso<strong>for</strong>ms, suggesting that clustered Pcdhs have a role <strong>for</strong> generating neural<br />

diversity <strong>an</strong>d org<strong>an</strong>ization in <strong>the</strong> brain. According to <strong>the</strong> previous reports, gene targeted mice<br />

lacking <strong>the</strong> Pcdhγ gene cluster showed neuronal loss by apoptotic cell death in <strong>the</strong> spinal cord<br />

<strong>an</strong>d died shortly after birth. To <strong>an</strong>alyze <strong>the</strong> function of Pcdhγ in neural development more detail,<br />

we generated mice with conditional alleles in which Pcdhγ proteins c<strong>an</strong> be null <strong>from</strong> specific cell<br />

types by Cre recombination. We crossed <strong>the</strong> conditional Pcdhγ mice with α-calmodulin kinase 2<br />

Cre (CaMK2Cre) tr<strong>an</strong>sgenic mice to selectively inactivate Pcdhγ proteins in excitatory neurons<br />

in <strong>the</strong> <strong>for</strong>ebrain. In <strong>the</strong> mut<strong>an</strong>ts, we detected apoptotic cells in <strong>the</strong>ir hippocampus, amygdala, <strong>an</strong>d<br />

piri<strong>for</strong>m cortex. Interestingly, apoptotic cells were neurons in which CaMK2 promoter would not<br />

work. These results suggest that Pcdhγ protiens regulate survival of neurons non-cell<br />

autonomously.<br />

Disclosures: Y. Itoga, None; S. Hamada, None; T. Hirabayashi, None; T. Yagi, None.<br />

Poster<br />

506. Neurogenesis II

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!