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When citing an abstract from the 2009 Annual - Society for ...

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mammali<strong>an</strong> Suppressor of Hairless (RBP-J, a component of <strong>the</strong> Notch signaling pathway) or/<strong>an</strong>d<br />

its paralogue RBP-L but retain <strong>the</strong>ir ability to <strong>for</strong>m dimeric complex with class-A bHLH<br />

proteins, had graded effects on retinal lamination <strong>an</strong>d cell specification. Notably <strong>the</strong> complete<br />

loss of interactions with RBP-J <strong>an</strong>d RBP-L abolished <strong>the</strong> activity of ectopic Ptf1a on amacrine<br />

<strong>an</strong>d horizontal cell differentiation.<br />

Toge<strong>the</strong>r, our results indicate that, in <strong>the</strong> chick retina, Ptf1a is sufficient to promote<br />

horizontal/amacrine cell genesis <strong>an</strong>d to inhibit g<strong>an</strong>glion cell differentiation while progenitors that<br />

misexpress Ptf1a retain <strong>the</strong>ir ability to progress through cell cycle. Our study demonstrates that<br />

Ptf1a acts as part of a tr<strong>an</strong>scription complex containing RBP cofactors, as previously suggested<br />

in <strong>the</strong> mouse retina.<br />

Disclosures: E. Lelievre, None; L. Houille, None; V. Brajeul, None; A. Slembrouck,<br />

None; S. Thomasseau, None; J. Sahel, None; O. Goureau, None; X. Guillonneau, None.<br />

Poster<br />

505. Cell Lineage <strong>an</strong>d Cell Fate Specification<br />

Location: South Hall A<br />

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 505.8/A8<br />

Topic: A.02.b. Cell lineage <strong>an</strong>d cell fate specification<br />

Support: Schram-Stiftung<br />

Wilhelm-S<strong>an</strong>der-Stiftung<br />

DFG<br />

Title: Hox genes in <strong>the</strong> pre-specification of sympa<strong>the</strong>tic <strong>an</strong>d parasympa<strong>the</strong>tic ciliary neuron<br />

progenitors<br />

Authors: L. HUBER, J. STUBBUSCH, M. FERDIN, *H. ROHRER;<br />

Max-Pl<strong>an</strong>ck Inst. Brain Res., 60528 Fr<strong>an</strong>kfurt/M, Germ<strong>an</strong>y<br />

Abstract: Autonomic neuron development is initiated by BMPs acting on neural crest cells in<br />

<strong>the</strong> primordia of sympa<strong>the</strong>tic <strong>an</strong>d parasympa<strong>the</strong>tic g<strong>an</strong>glia. BMPs induce different patterns of<br />

tr<strong>an</strong>scription factors in sympa<strong>the</strong>tic <strong>an</strong>d parasympa<strong>the</strong>tic ciliary g<strong>an</strong>glia, which indicates intrinsic<br />

differences between BMP-responsive progenitors in different autonomic g<strong>an</strong>glia (Müller <strong>an</strong>d<br />

Rohrer, 2002).<br />

Here we have addressed <strong>the</strong> molecular basis of this intrinsic difference <strong>an</strong>d pre-specification

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