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When citing an abstract from the 2009 Annual - Society for ...

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Poster<br />

510. Regeneration: PNS<br />

Location: South Hall A<br />

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 510.24/B57<br />

Topic: A.08.b. Regeneration: PNS<br />

Title: Schw<strong>an</strong>n cell-like differentiation of adipose-derived mesenchymal stem cells<br />

Authors: *C. RADTKE 1,2 , P. M. VOGT 2 , B. SCHMITZ 2 , M. SPIES 2 , J. D. KOCSIS 1 ;<br />

1 Yale Univ. Sch. Med., West Haven, CT, CT; 2 Dept. of Plastic, H<strong>an</strong>d <strong>an</strong>d Reconstructive<br />

Surgery, H<strong>an</strong>nover Medicla Sch., H<strong>an</strong>nover, Germ<strong>an</strong>y<br />

Abstract: Recent attention has focused on <strong>the</strong> potential use of bone marrow cells which contain<br />

mesenchymal stem cells to repair <strong>the</strong> CNS because it contains populations of precursors that are<br />

multipotent <strong>an</strong>d c<strong>an</strong> differentiate into a number of cell types including bone, cartilage <strong>an</strong>d muscle<br />

cells. Mesenchymal stem cells c<strong>an</strong> be isolated <strong>from</strong> adipose tissue of <strong>the</strong> adult rat. These cells<br />

have been shown to differentiate after appropriate induction into various cell lines, e.g. fat cells,<br />

muscle, cartilage <strong>an</strong>d bone as mesenchymal stem cells <strong>from</strong> bone marrow. In addition,<br />

mesenchymal stem cells s derived <strong>from</strong> bone marrow were able to tr<strong>an</strong>sdiffentiate into glia-like<br />

cells. The question we asked was if adipose derived stem cells c<strong>an</strong> also be differentiated <strong>an</strong>d<br />

tr<strong>an</strong>sdifferentiate into glia-like cells. Adipose-derived mesenchymal stem cells were isolated<br />

<strong>from</strong> adult rats, cultured <strong>an</strong>d differentiation was induced by basis fibroblast growth factor <strong>an</strong>d<br />

epidermal growth factor in vitro. Morphology <strong>an</strong>d fur<strong>the</strong>r immunocytochemical markers of<br />

differentiated cells <strong>an</strong>d undifferentiated cells were compared. Here, we demonstrate that adiposederived<br />

mesenchymal stem cells <strong>for</strong>m nestin-positive neurosphere-like structures after induction.<br />

Fur<strong>the</strong>r differentiation leads to signific<strong>an</strong>t morphological ch<strong>an</strong>ges <strong>an</strong>d to expression of <strong>the</strong><br />

characteristic Schw<strong>an</strong>n cell marker S100 <strong>an</strong>d p75 nerve growth factor receptor <strong>an</strong>d <strong>the</strong><br />

characteristic marker GFAP <strong>for</strong> astrocytes. The simult<strong>an</strong>eous expression of characteristic glia<br />

marker of <strong>the</strong> central nervous system <strong>an</strong>d <strong>the</strong> peripheral nervous system is <strong>an</strong> exceptional feature<br />

of olfactory ensheathing cells with unique properties regarding remyelination <strong>an</strong>d enh<strong>an</strong>cement<br />

of axonal regeneration. These results indicate that adipose-derived mesenchymal stem cells<br />

grown as neuropsheres c<strong>an</strong> differentiate morphological <strong>an</strong>d functional into <strong>an</strong> Schw<strong>an</strong>n cell-like<br />

glia population.<br />

Disclosures: C. Radtke, None; P.M. Vogt, None; B. Schmitz, None; M. Spies, None; J.D.<br />

Kocsis, None.

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