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prohormones in this model, <strong>the</strong> multitude of final peptide <strong>for</strong>ms arising <strong>from</strong> alternative splicing,<br />

<strong>an</strong>d extensive posttr<strong>an</strong>slational processing of <strong>the</strong> final peptides. Mass spectrometric<br />

measurements at <strong>the</strong> peptide level offer signific<strong>an</strong>t adv<strong>an</strong>tage as <strong>the</strong>y allow detailed high<br />

throughput characterization of structurally similar gene products without <strong>the</strong> requirement of a<br />

priori knowledge of <strong>the</strong> identity of relev<strong>an</strong>t peptides.<br />

In this study we use label-free mass spectrometry (MS) based workflow <strong>for</strong> spatial <strong>an</strong>d temporal<br />

<strong>an</strong>alysis of peptides <strong>from</strong> pl<strong>an</strong>ari<strong>an</strong>s regenerating <strong>an</strong>terior <strong>an</strong>d posterior tissues <strong>an</strong>d characterize a<br />

subset of peptides potentially associated with <strong>the</strong> regeneration process. Peptides were measured<br />

in individual samples of <strong>an</strong>terior <strong>an</strong>d posterior regenerates collected at various time points postamputation<br />

by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry<br />

(MALDI-TOF MS). Principle component <strong>an</strong>alyses (PCA) of complex peptide profiles in<br />

regenerating tissues revealed temporal trends in peptide ch<strong>an</strong>ges taking place during 72 h postamputation.<br />

A subset of novel putative peptides that exhibit time-dependent ch<strong>an</strong>ges were<br />

characterized by liquid chromatography <strong>an</strong>d t<strong>an</strong>dem mass spectrometry (LC/MS-MS) with <strong>the</strong><br />

aid of bioin<strong>for</strong>matic tools <strong>an</strong>d <strong>an</strong> in-house database of prohormones expressed in S.<br />

mediterr<strong>an</strong>ea. Differentially detected peptides have been found to originate <strong>from</strong> several novel<br />

neuropeptide prohormones.<br />

Disclosures: E.V. Rom<strong>an</strong>ova, None; J.J. Collins, None; P.A. Newmark, None; J.V.<br />

Sweedler, None.<br />

Poster<br />

507. Stem Cells: Basic Biology II<br />

Location: South Hall A<br />

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 507.7/A46<br />

Topic: A.03.b. Stem cells: Basic biology II: Expression patterns, properties, <strong>an</strong>d responses<br />

Support: Division of Intramural Research, NINDS<br />

Title: Comprehensive phenotyping reveals <strong>the</strong> true identities of neural stem cells <strong>an</strong>d<br />

intermediate progenitors <strong>an</strong>d resolves <strong>the</strong>ir ontogenetically ch<strong>an</strong>ging lineage progressions<br />

underlying cortical neurogenesis<br />

Authors: *D. MARIC, Y. H. CHANG, J. L. BARKER;<br />

NINDS/NIH, Be<strong>the</strong>sda, MD<br />

Abstract: We used immunolabeling with a multitude of developmentally relev<strong>an</strong>t markers to<br />

phenotype neural stem cells (NSC) <strong>an</strong>d intermediate progenitors (IP) emerging during <strong>the</strong>

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