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When citing an abstract from the 2009 Annual - Society for ...

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induced c-FOS cells was signific<strong>an</strong>tly increased if <strong>an</strong>imals were active due to FAA or awakened<br />

by <strong>the</strong> experimenter. The effect of activity is supported by a positive correlation between <strong>the</strong><br />

number of wheel revolutions <strong>an</strong>d <strong>the</strong> number of c-FOS cells in <strong>the</strong> SCN. Surprisingly, c-FOS<br />

was not observed in GRP cells, a subpopulation of SCN cells that respond to light during <strong>the</strong><br />

subjective night. In <strong>the</strong> absence of a light-pulse, <strong>the</strong> <strong>an</strong>imals in <strong>the</strong> Awakened condition had<br />

more shell (not core) c-FOS compared to <strong>the</strong> Undisturbed condition. There was no difference<br />

between FAA <strong>an</strong>d Awakened groups in c-FOS in ei<strong>the</strong>r <strong>the</strong> presence or absence of light. These<br />

results suggest that <strong>the</strong> SCN responds to light during <strong>the</strong> subjective day <strong>an</strong>d that activity, whe<strong>the</strong>r<br />

endogenously org<strong>an</strong>ized or exogenously driven, enh<strong>an</strong>ces this response.<br />

Disclosures: E. Rodriguez, None; J. LeSauter, None; R. Silver, None.<br />

Poster<br />

574. Entrainment <strong>an</strong>d Phase Shifts: Neurons, Tr<strong>an</strong>smitters, Genes, <strong>an</strong>d Proteins<br />

Location: South Hall A<br />

Time: Tuesday, October 20, <strong>2009</strong>, 8:00 am - 12:00 noon<br />

Program#/Poster#: 574.5/FF55<br />

Topic: E.08.a. Entrainment <strong>an</strong>d phase shifts<br />

Support: UMDNJ foundation<br />

Title: Membr<strong>an</strong>e gu<strong>an</strong>ylate cyclase in circadi<strong>an</strong> rhythms<br />

Authors: *V. VENKATARAMAN 1 , R. SWANSON 1 , A. KRISHNAN 1 , T. DUDA 2 ;<br />

1 Cell Biol., UMDNJ, Strat<strong>for</strong>d, NJ; 2 Salus Univ., Elkins Park, PA<br />

Abstract: Cyclic GMP plays a critical role in setting <strong>the</strong> phase in circadi<strong>an</strong> rhythms. However,<br />

<strong>the</strong> mech<strong>an</strong>ism by which it is regulated is unclear. The results <strong>from</strong> this study suggest that<br />

membr<strong>an</strong>e gu<strong>an</strong>ylate cyclase is <strong>the</strong> source <strong>an</strong>d that its activity is regulated by <strong>the</strong> neuronal<br />

calcium sensor protein neurocalcin δ. This would also enable a tight coupling of <strong>the</strong> system to<br />

calcium, which is already <strong>an</strong> established regulator of generation <strong>an</strong>d mainten<strong>an</strong>ce of circadi<strong>an</strong><br />

rhythms.<br />

Disclosures: V. Venkataram<strong>an</strong>, None; R. Sw<strong>an</strong>son, None; A. Krishn<strong>an</strong>, None; T. Duda,<br />

None.<br />

Poster

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