Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
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8. Colony hybridizalion sc.eening ot transtomed baderia . . . .. . 42<br />
9, F6striclion enzyme analysis ol positivo clones . .,.... . .,.46<br />
ro. DNA sequence analysis ot <strong>H4</strong> Promoter conslrucls I . -... ... -49<br />
11 ONA sequonce analysis ol <strong>H4</strong> Promoler conslrucls ll . .,,... -. -. 51<br />
12. ONA sequence anatsis ol <strong>H4</strong> promotsr construcls lll . ...... 53<br />
13. Schematic diagr€m illustating the prsparalion of plasmld probe<br />
15.<br />
17.<br />
14.<br />
20.<br />
21.<br />
22.<br />
23.<br />
24.<br />
25_<br />
lo. use in gelshm assays -...............-..... -.. -. .. .. -. -. -. -.... ... - 60<br />
MoFhology ol human H6La s3 ceMcal carcjnoma cels (A),<br />
rat osteosarcoma (Ros) c€||s (B) and rcl ostsoblasl (RoB)<br />
cerls (c) . .<br />
ProdJdon ol <strong>H4</strong> promo€ CAT slabl€ c€lllines<br />
Aralys s ol srabecell ines<br />
Souhern analys s ol staoly kansleded cell l nes .<br />
CAT acttrity ot stably tr&slsclsd <strong>H4</strong> Promoler CAT c€nslructs -<br />
Sy'cnroniarol olsldble c6llli1es ...... - -<br />
Pholograph ol synchro.ized HeLa cels a hours alter release<br />
trom sec€nd rym'd ne blocl<br />
lsolato. ot n-c el<br />
Nucled run-on lranscription analysis ol synchronized slabl€ c€ll<br />
lsolarion o'toralc€ll-lar RNAlron synch'on zod ce ls<br />
Electrophor€ic mobilily shilt assay condi ons lor H N F P, M & O .<br />
Mutalional analysis ol <strong>H4</strong> site ll protein-oNA inleractions . . . .<br />
Mutational afalysis ol <strong>H4</strong> sit6 ll prolein oNA inleractions . . .,.<br />
79<br />
a6<br />
8a<br />
93<br />
95<br />
99<br />
'106<br />
110<br />
119<br />
121<br />
127
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