Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
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Crct C@nd d H. GNf,'/MAi@<br />
'2izE:Ce<br />
Materials and Methods<br />
MUTAGENESIS <strong>OF</strong> SITE II BYi'OLECULAR CLONING<br />
lh€ hisron€ <strong>H4</strong> gens promorg (F19. 5) us€d in this sludy is detivsd<br />
lrom plasmad FOIOa which was cloned in this laborarory by Si6na er at<br />
(1983). The 0.2 kb prcmoter ol d|e <strong>H4</strong>-Fo108 gene was llsed lo lhe caT<br />
g€ne by or. Anna Aams€y-Ewing (Fam36y'Ewng €t a/., 1994) Th€ res!fting<br />
4.55 kb iusion construct was desiEnal€d pFOICaCAT; this plasmid repf6s€nts<br />
rh6 witd type conEttuct, which was used hroughod ltE mutag€nees studi€s'<br />
Muranr p.omolsr c!.rsLucls co.tanhg th6 c T rcpo.ler 9en6 w€.€ Pr6pded<br />
by an origonucl€oridE cassetle apprcachs (Frgs, 5,6 a.d 7) .<br />
Plasmd us€d in lhs studY contalns the <strong>H4</strong> proximaL prornol€f ol lh€<br />
pFOlOa gen€ and spans nuclsolidos (nt) '240 to _38 (rslativs ro $e<br />
a
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