Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...
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Selection ol cell linss contalning chimeric <strong>H4</strong> promoter CAT repoder<br />
geneconstrucls.,..,,,.._...........,........._..._..._..,...,..,,,...,...,..,,...,..........73<br />
Prepdarion oi glycerot stocks ior stabl€ c€ll lin6s ...._..._..............,..,, 76<br />
Soufhsrn analysis .....,.,.....-... ......-..-...--..-...-.......-.....,...,....,_,,............ Z6<br />
lsolalionofgeriomicDNA...,....,,..,...,..._.....,,,.,,.,,,.,,.,..................,...,.76<br />
Transf€r ot ONA to a msmbrarous sotid suppoft .,...,................._..... g1<br />
ttepar€tion ol €diolabeiled prcb€ ror Sourhem hybridiza on ......... 83<br />
Slafdardprocodurelorhybridizarion.............................................g4<br />
synchronizatonolsrabteHeLacelttin€s..........................................85<br />
suspension cullure of H€La s3 cell lines with integrat€d reponer<br />
genos ...................._............................_........................................... 85<br />
Doublelhymidn€blocksynchronizarionproced!rc.........................91<br />
Monitonng cell syncnrcny ..................-..,...,,...,.... .... .................,...._ 92<br />
O€terminarion ol ONA synthesis ,..,..,..,,..,....,............_...._..._..,..,,....,.. 97<br />
Nucl€arrun on lranscription: tsotation ofNuct€i ,.,,,..,,.............,..,. 97<br />
rsoralion oinlcei ................_......,..,,...97<br />
TEnscription reaction ......._....._.,,,....... 99<br />
DNASlot-8|oi.............................................................................. 102<br />
Pr€p8ration oi hybrldization membranas using ihe manlotd I sot<br />
lsolation ol totatcettutar RNA ton mamalian c6lts ........._.....,,.... .... i05<br />
Fibonuclease protection assay ............................................... ...... 109<br />
/, vr't o t anscripljon reaction ........,..,........_..._..._......._...... .....,.._..... 1 12<br />
Hyb.idiairon ol RNA transcripis and sampte RNA ,,,._.........._...,.,,,, j j 2
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