Page 1 \ ?^p 6r.1 CELL CYCLE CONTROL OF HUMAN H4 ...

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A2i2 t: cen crcIo cohrt,!.t ul G.re ft@tdut expression ol hi$on6 gene va ants aloch€rnisrry,28:5318-5322 SHERWOOO, P. W,TSANO, S V ANO OSLEY, M A'' 1993 characle'izalon ol HlRl and HlR2, iwo genes reqLrired lol regulation 0l hislone gene lBnscipnon h saccharornyc€s cercvisias Mol ce/' a'o' . 13: 28_38 SIERFA, F., STEIN, 7069-7046, G. SITTMAN, D 8., GFAVES' AND STEIN, J., 1983. human <strong>H4</strong> histone gone SIVE, H L, HEINTZ, N., ROEDEF' R.G' gene expression during the HeLa Mo| cat, Biol-, 4: 2723-2734 SIVE H.L, HEINTZ, N, AND FOEDER' ebmenls are requir€d lor maxrmal struclure and ,n vrllo' Noc/. Actds. Fss., 11: MARZLUFF, w.F., 1983. Hidone mRNA ar |he level ol aanscriplion and mRNA scr" usq, ao: 1849'18:€ hi$one HzBgen€ Mol ce/i 81Ol 3329-3340. '6i 1s84. Fegulaton ot human hslone cell c-ycle requnes protein svnlhes's R,G., 1986. MlllLple seqlence ,n vttro t.ansdiption oi a human SOuTHERN, PJ ANDBERG P. 1982 Ttanslodalion 'l mammaL6n cers ro a.trbrotic resistance wlih a bacterial ge'e lnder conlfol ol lhe SV'10 eany region promorer' J Mo', Appt Genet 1:327'341 STAUBER C ANO SCHUiiPERLL. O , 1988 3 Pro'essr'g 'i pre mBNA oravs 185
,rit 9: Ceu Cycle C.htot ot Ha C.n6 f@&tiotim a major rc16 in prolitaration-dependenl reg'rlaiion ol histone gen8 expresslon lvuct Aclds Bes., 16: 9399'9414. STEIN, G.S., SIEfiM, F., STEIN, J,!-, PLUMB, M., MABASHI. F, 1984, Organizallon and gxprsssion ot human hision6 qenes h: Htslote genes (eds. G.s. st€in. J.l. st€in and wF. Marzlufi. J.-) P 3S7-455 John Wiley and Sons, NewYork. STEIN, G-, LIAN, J., STEIN, J., BRIOGS, N., SHALHOIJB, V., WFIGHT, '
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\ ?^p 6r.1 CELL CYCLE CONTROL OF HU
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In tho narn€ of Alah, olt G6cloua
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Acknowl6dg6msnts Contents Abstract
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Selection ol cell linss contalning
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ACKNOWLEDGEMENTS
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I would also lkg ro rhank Dr. Pafic
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ABSTRACT
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i.leraclion elem€nt, sil6 ll, Thr
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8. Colony hybridizalion sc.eening o
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INTRODUCTION
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t t F: con cda c@tot d H. G@ r@Mbn
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Azh F: C6n Ca/cro C@t.t ot H. G.n.
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Aziz f: Celi Ctcle Cotutol ol H4 Ae
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= 2. q t6l tl9t E L:I la-lf titl I.
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En F. Ce Cycl. Cantd d h. Gft ra.sc
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,r1z F Cett cy.te connot ol H4 Gene
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A2i2F: Cettcdt cotubtot e@IrNiotid
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Ntz F: Ce Cwte Contd d 114 Gm tansc
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t- s- i;> f z x + n q e o, { T T z
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AzzF cett Crct. ContolatH4Gen ldn*t
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a2i2 F cdt c$t. connd d H4 Gm Tdnsc
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o rl$orrE GEIE FEEI{IXU =; FrN -!r
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!e 3lhllrllon c.I cycl. control aa
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A.E F: Cet CEl. Cotuot ot H4 t3.M f
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Crct C@nd d H. GNf,'/MAi@ '2izE:Ce
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9C| -= = , o:li?eiP :< :::? il il 7
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s.tudi. dhcrm or do"rn! -l /4 I I I
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aziz F: cel crd. cotuot d 81 cd. fn
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a2lz F: celt cyd. c.ntol d H4 G@. f
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A2iz F Cett cycta contat al Hl Gene
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E2 F. cen crct. Connat al H4 c*e Ta
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Colony hybridization 4a aa a a
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,dd f . c.t cfi|e cotud d H4 cd'e f
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Restriction Analysis Of Mutants e'b
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,d, F: ccl crc!. cqfrol ot H10d fdt
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AtIzF: Cd CJ.L Cdtuotol BaGq:€lat
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,.8 F: 6t W @tlol o, rk 0.,t rn',df
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ldD F: Cett CrL Cotuot at t14 G* Iw
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A2E F. cdt crcto Cantat at Ha Gqa r
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Aziz F: Cdl Ced. Cd*ol ol H4 Gse ra
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o E o !!tH I rtog 5l €s c_g - t-
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kir F: cettctttd contal at H4 cen.
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arE F: CeI Crct Cotuatat 114 G40 fa
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c A B e'l' t i _ f '', \J..$.'
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Nz F: Cel Ctd. Ctuol at h4 Cq. faBd
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Aziz F: Celt Cyct. Contd d H4 6@e l
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azE F. celt cy.b contol al H4 Gene
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A Geneticin (G4'18) rssistanl HeLa
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NizF: Cen qcb cttuat al tu G@ ft|tl
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M F: Cd W W.l h. 4.. 7'.t'll'Pn'd F
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'dit F: ce qcle cotuot ol Ha Gm ra@
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M. F: Ce|| Crd. Cqnrot ot Ha Gerc T
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azi. F: cetl cttcL ctu.t or Ha Gtu
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kiz F: c.lt
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ktz F. cen ctct6 ctuat at tu 6e rft
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Azi2 F: Cen Cvcte Contot .t H4 Gere
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I 200000 1mmo DNA Synth.3l3 In Sync
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High mitotic index alter relsase tr
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ND F Cen atct6 cannot ot B4 6ene rr
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NE F: Cell C6t cotuat .t H4 cffi fh
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A22 F: Cett cytl. Connd d H4 64. rn
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hnF cet cyttd cannot ot B4 6end ltu
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Nuclear Run-on TranscriPlion wr Fol
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NlzF Cett cr.t. canuat oI H4 Gene f
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RNA isolated f rom cell synchrony H
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A2iz F: c.u cwL contat al Ha GeN f@
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RESULTS
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trb F: cett clcL cotuot al H4 Gen.
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M2F:Co Crrt6 Cdrtot ot H4G.n. fn.pi
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HiNF-tvl HiNF-P HiNF-D random DNA p
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andom DNA poly G/C competitor oligo
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a2i2 F: ceu ctcte contol al H4 Gene
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aziz F: c.ll crd. cdtd 0t H4 Gene t
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compeutor oligonucleolides: Site ll
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A1i2 F: Ceu a:rcte cotuot ot H1 G@
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andom DNA poly G/C competilor oligo
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EEF:Ce Olcta Cont t ol114 Gene l@ns
Page 155 and 156: , F: cal qcb cotfrtl d rh 9.n ftuaf
Page 157 and 158: kL E d N. Cdfr.t ol k e.rE ltlr&tu
Page 159 and 160: Mt F: Cett C\.1e Catud cn Ha Ger. I
Page 161 and 162: A.*F: Cen q.h cdtuotot Ha 6an ranfl
Page 163 and 164: 3!j{99!caaw arir c",. r_*rooo FI9 3
Page 165 and 166: I -a2b F: cel cyde c@xol d B1 aa@ h
Page 167 and 168: -= GAPDH mRNA = = 012 66 hours afle
Page 169 and 170: Fq *' I E t li! lil tlr ilt Frlr li
Page 171 and 172: (! (! t- (Y) F o 'F (E d z l t52 +
Page 173 and 174: E Comparison ol TgT9 ratios lor cAT
Page 175 and 176: Azd F: Cd Ctcle Ca\ttol ot ltt Gtuf
Page 177 and 178: :tE &I qiE{, ccaoi oftaa Cer tir fi
Page 179 and 180: kEE@q.l.co''o,a,tl,od,',rutg/bt!,.
Page 181 and 182: ibEc|,Otd.c!,'t,dlHao'tllep,rcl Fg.
Page 183 and 184: DrscussroN
Page 185 and 186: I azz F Con CYb Cnror ot H4 G@e ntn
Page 187 and 188: ,@2 F. Cdt CEle Cotud .l U1 G.N fan
Page 189 and 190: l NE P C.l qE/€ Cotud d H4 Gw f@f
Page 191 and 192: aznF:couct l. cdtold H16@f@tioti@ s
Page 193 and 194: ari2 F: c.t] cyctd contnt ot H1 Ger
Page 195 and 196: Atiz F. Cdl cycl6co.ttol d H4 Gene
Page 197 and 198: l A2izF C.ttctct (onntt ot a. calo
Page 199 and 200: azi. F: cei c\cb cotuot d H4 G@ fon
Page 201 and 202: NizF Can a$t. c@tot ot H4 G.n fftNc
Page 203 and 204: Atiz F: Catl Cvcte contot ol H4 6.n
Page 205: Aztz F a.tt ctcte conr ot .t Hl 6en
Page 209 and 210: tai. F: ceu cycte cantat ot ,r1 Gen
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