Ni. F. Cer qib chhnat ol <strong>H4</strong> G6ne fanetptd ORAAENT, 8., BODE, C. ANO DOENECKE, D., 1993, SITCIUiE ANd exp.ession ot the mous€ tBsticlld Hl hisron€ gen6 (H1t). a,ocrr,n. Biapl,ys. Acta, 121 6: 31 1 -313, DMBENI. S.. KARDALINOU, E. expression ot lhe human Gen6, 103: 263-264. AND DOENECKE, D-, 1991, Slructur€ and g€n6 encodi^g rosriorlar H1 histone (H1r). OYNAN, WS,, AND TJ|AN, R., 1983. tsotation ot {ransc ption iacto.s thai disc minare balw€en difiorent promolers ,6clgnized by RNA porym6Gs6 tl. C€l, 32:669680. EHR]NGER, A.. OENISON, S.H. AND i,1AY, G.S., 1990. S€quencg oGanizarion and €rpression oa th€ core histone Gen- Cenel , 222. 4 16-424. genes ot Aspetgillus nidutans. ttal. EILEAS A., BOUTENFA, H., TRIESE S., AND DOENECXE, D, 1994. ROIE O' A disral promoter eleme in th€ S.phase controt ot rhe human H1.2 hisbne g€ne rransciprion. Eu t. J. Bi@hn., 223: $7 -57 4. EMMEF, M,. OE CROMBFUGGHE, 8,, PASTAN, I., ANO PEFLMAN, F, 1970, Cyclc AMP receptor prolein ol E. co/i: ils role in the synthess ol inducible enzymes. Proc. Nad Acad. sci US4, 66: 480,487. FLETCHER, C., HEINTZ, N. ANO BOEOER, G.R., 1987. Puriticaton and chafact€nzalion of OTF.I a transcription lacto. regu aling ceLt cycte expression oi a human histone H2b gene. ce|,51:773 7a1 't73
Atiz F. Cdl cycl6co.ttol d <strong>H4</strong> Gene ftunscr ti@ GILBEFT. W, AND MULLER-HILL 4., 1966. lsa::r cn ot 'ho lac r€prsssor P.oc, sct us4, s6: 1a91ra9a. 'Var. ^cad- GOMEZ.GUADFAOO, A,, FOUSSEAU, S,, FENAUD, J. AND FUIZ.CARFILLO, A., 1992. Fep.sssion ot tho H5 hlstono gene by a iador lrom erythrocyles ihat binds to the r€gion ol lranscriPton inillation EMAO J., 1t:1857-1866, GORMAN, C.M., M<strong>OF</strong>FAT, L.F. ANO HOWARD, 4.H., 1942. RSCOMbTANI g€nom€s which expross chlorampheaicol ac6lYl.ransle.as9 in mammaliancells.Mol. C€ll. 8,o/,, 2: 10441051 GAEENBEFG, tM.E. AND ZIFF, E,8.. 1984. Stimuia on ol3T3 c€lls induces l.ansciplion ol the cJos proto-onctEene. ,'ualu.6, al 1 : 433_4la GFIMES, S. F., WOLFE, S. A., ANDEBSON, J. V,, STEIN, G.S', ANO STEIN' J.L, 1990. StrucuraL and tlndional analysis ol the rat lestis-speciiic hlsrone Hlr gene. J. cel, Biachen.,44 1.17 GRIMES, S R., woLFE, s. A. AND KOPPEL, D a.,1992. T€mpo.al coiielatLo. between lhe appearance ol les s_specifc DNA bindlng proleins and the onset ol tansc pion ol lhe restis specific hislone Hll gene E p cell Res., 2o1 : 2 16-224. GROSSCHEOL R. ANO BInNSTIEL, l\,1L., 1S€0. Spac€r DNA sequences opsteam ol lhe T.A.T-A-A.A.T'A seque.ce are essenlla lor promono' or H2A hrstone gene rranscrirnlon in viva Prcc Natt Acad. sct usa
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\ ?^p 6r.1 CELL CYCLE CONTROL OF HU
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In tho narn€ of Alah, olt G6cloua
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Acknowl6dg6msnts Contents Abstract
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Selection ol cell linss contalning
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ACKNOWLEDGEMENTS
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I would also lkg ro rhank Dr. Pafic
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ABSTRACT
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i.leraclion elem€nt, sil6 ll, Thr
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8. Colony hybridizalion sc.eening o
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INTRODUCTION
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t t F: con cda c@tot d H. G@ r@Mbn
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Azh F: C6n Ca/cro C@t.t ot H. G.n.
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Aziz f: Celi Ctcle Cotutol ol H4 Ae
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= 2. q t6l tl9t E L:I la-lf titl I.
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En F. Ce Cycl. Cantd d h. Gft ra.sc
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,r1z F Cett cy.te connot ol H4 Gene
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A2i2F: Cettcdt cotubtot e@IrNiotid
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Ntz F: Ce Cwte Contd d 114 Gm tansc
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t- s- i;> f z x + n q e o, { T T z
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AzzF cett Crct. ContolatH4Gen ldn*t
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a2i2 F cdt c$t. connd d H4 Gm Tdnsc
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o rl$orrE GEIE FEEI{IXU =; FrN -!r
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!e 3lhllrllon c.I cycl. control aa
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A.E F: Cet CEl. Cotuot ot H4 t3.M f
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Crct C@nd d H. GNf,'/MAi@ '2izE:Ce
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9C| -= = , o:li?eiP :< :::? il il 7
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s.tudi. dhcrm or do"rn! -l /4 I I I
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aziz F: cel crd. cotuot d 81 cd. fn
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a2lz F: celt cyd. c.ntol d H4 G@. f
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A2iz F Cett cycta contat al Hl Gene
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E2 F. cen crct. Connat al H4 c*e Ta
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Colony hybridization 4a aa a a
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,dd f . c.t cfi|e cotud d H4 cd'e f
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Restriction Analysis Of Mutants e'b
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,d, F: ccl crc!. cqfrol ot H10d fdt
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AtIzF: Cd CJ.L Cdtuotol BaGq:€lat
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,.8 F: 6t W @tlol o, rk 0.,t rn',df
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ldD F: Cett CrL Cotuot at t14 G* Iw
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A2E F. cdt crcto Cantat at Ha Gqa r
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Aziz F: Cdl Ced. Cd*ol ol H4 Gse ra
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o E o !!tH I rtog 5l €s c_g - t-
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kir F: cettctttd contal at H4 cen.
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arE F: CeI Crct Cotuatat 114 G40 fa
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c A B e'l' t i _ f '', \J..$.'
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Nz F: Cel Ctd. Ctuol at h4 Cq. faBd
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Aziz F: Celt Cyct. Contd d H4 6@e l
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azE F. celt cy.b contol al H4 Gene
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A Geneticin (G4'18) rssistanl HeLa
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NizF: Cen qcb cttuat al tu G@ ft|tl
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M F: Cd W W.l h. 4.. 7'.t'll'Pn'd F
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'dit F: ce qcle cotuot ol Ha Gm ra@
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M. F: Ce|| Crd. Cqnrot ot Ha Gerc T
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azi. F: cetl cttcL ctu.t or Ha Gtu
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kiz F: c.lt
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ktz F. cen ctct6 ctuat at tu 6e rft
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Azi2 F: Cen Cvcte Contot .t H4 Gere
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I 200000 1mmo DNA Synth.3l3 In Sync
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High mitotic index alter relsase tr
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ND F Cen atct6 cannot ot B4 6ene rr
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NE F: Cell C6t cotuat .t H4 cffi fh
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A22 F: Cett cytl. Connd d H4 64. rn
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hnF cet cyttd cannot ot B4 6end ltu
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Nuclear Run-on TranscriPlion wr Fol
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NlzF Cett cr.t. canuat oI H4 Gene f
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RNA isolated f rom cell synchrony H
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A2iz F: c.u cwL contat al Ha GeN f@
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RESULTS
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trb F: cett clcL cotuot al H4 Gen.
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M2F:Co Crrt6 Cdrtot ot H4G.n. fn.pi
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HiNF-tvl HiNF-P HiNF-D random DNA p
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andom DNA poly G/C competitor oligo
- Page 143 and 144: a2i2 F: ceu ctcte contol al H4 Gene
- Page 145 and 146: aziz F: c.ll crd. cdtd 0t H4 Gene t
- Page 147 and 148: compeutor oligonucleolides: Site ll
- Page 149 and 150: A1i2 F: Ceu a:rcte cotuot ot H1 G@
- Page 151 and 152: andom DNA poly G/C competilor oligo
- Page 153 and 154: EEF:Ce Olcta Cont t ol114 Gene l@ns
- Page 155 and 156: , F: cal qcb cotfrtl d rh 9.n ftuaf
- Page 157 and 158: kL E d N. Cdfr.t ol k e.rE ltlr&tu
- Page 159 and 160: Mt F: Cett C\.1e Catud cn Ha Ger. I
- Page 161 and 162: A.*F: Cen q.h cdtuotot Ha 6an ranfl
- Page 163 and 164: 3!j{99!caaw arir c",. r_*rooo FI9 3
- Page 165 and 166: I -a2b F: cel cyde c@xol d B1 aa@ h
- Page 167 and 168: -= GAPDH mRNA = = 012 66 hours afle
- Page 169 and 170: Fq *' I E t li! lil tlr ilt Frlr li
- Page 171 and 172: (! (! t- (Y) F o 'F (E d z l t52 +
- Page 173 and 174: E Comparison ol TgT9 ratios lor cAT
- Page 175 and 176: Azd F: Cd Ctcle Ca\ttol ot ltt Gtuf
- Page 177 and 178: :tE &I qiE{, ccaoi oftaa Cer tir fi
- Page 179 and 180: kEE@q.l.co''o,a,tl,od,',rutg/bt!,.
- Page 181 and 182: ibEc|,Otd.c!,'t,dlHao'tllep,rcl Fg.
- Page 183 and 184: DrscussroN
- Page 185 and 186: I azz F Con CYb Cnror ot H4 G@e ntn
- Page 187 and 188: ,@2 F. Cdt CEle Cotud .l U1 G.N fan
- Page 189 and 190: l NE P C.l qE/€ Cotud d H4 Gw f@f
- Page 191 and 192: aznF:couct l. cdtold H16@f@tioti@ s
- Page 193: ari2 F: c.t] cyctd contnt ot H1 Ger
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- Page 199 and 200: azi. F: cei c\cb cotuot d H4 G@ fon
- Page 201 and 202: NizF Can a$t. c@tot ot H4 G.n fftNc
- Page 203 and 204: Atiz F: Catl Cvcte contot ol H4 6.n
- Page 205 and 206: Aztz F a.tt ctcte conr ot .t Hl 6en
- Page 207 and 208: ,rit 9: Ceu Cycle C.htot ot Ha C.n6
- Page 209 and 210: tai. F: ceu cycte cantat ot ,r1 Gen