Research Group - IPK Gatersleben

Abteilung Genbank/Department of Genebank
Fig. 14: Example of two records
of Differential Scanning Calorimeter
measuring heat flow in
shoot tips of Solanum tuberosum
L. “Désirée”. Samples were
cooled from 0 to -150 °C and
afterwards rewarmed from -150
to 0 °C, both with a speed of
10 °C/min. Green line: control
without cryoprotection. High
water contents are documented
by the crystallisation and melting
peaks. Blue line: sample after
cryoprotection with PVS (0.4 M
sucrose + 30 % glycerol + 15 %
ethylene glycol + 15 % DMSO) for
30 min. The cryoprotected tips
exhibited glass transition (Tg) at
-41.59 °C (magnified part of the
record in the inlay marked by red
lines). Transfer of the cell solution
from freezable water to highly
viscous solution being able to
form stable glasses is one of the
main principles of cryopreservation.
Pictures below the diagram:
Shoot tip isolated from an in vitro
plantlet of S. tuberosum “Désirée”
prior to cryopreservation
(left); regenerated shoot eight
weeks after rewarming (right)
(Photo: A. Kaczmarczyk).
to 1,433 accessions of garlic and 460 of shallot. The DNA
isolation was performed in collaboration with the research
group Experimental Taxonomy. The project-based
cryopreservation activity comprises 40 accessions in preparatory
in vitro propagation and three accessions in final
cryopreservation. For virus elimination five accessions
were sent to the partners. The garlic image database was
transferred into the group from the research group Plant
Data Warehouse (C. Zanke, G. Matzig, J. Keller, F. Blattner,
T. Funke, U. Scholz, M. Lange).
46
Collaboration
Within the Institute:
Dept. of Genebank, Research Group Genome Diversity;
Prof. A. Graner;
Dept. of Genebank, Research Group Genebank Documentation;
Dr. H. Knüpffer, M. Oppermann;
Dept. of Genebank, Research Group Plant Data Warehouse;
Prof. I. Große, T. Funke, S. Weise;
Dept. of Genebank, Research Group Resources Genetics
and Reproduction; Dr. A. Börner;