Research Group - IPK Gatersleben

Abteilung Genbank/Department of Genebank
Research Group:
External Branch “North”
Head: Dr. Klaus J. Dehmer
Scientists
IPK financed
Willner, Evelin (P)
Goals
Collection, conservation, characterisation, evaluation,
service, documentation and research activities of/on
potato, oil and forage crop plant genetic resources.
Research Report
Within three collections − clonally propagated genotypes
of Andean or equatorial origin (AKS; 490 entries, mainly
seven cultivated species), ,590 cultivars and breeding
lines of Solanum tuberosum ssp. tuberosum (KKS, vegetatively
propagated) and ,859 accessions of 139 wild and
cultivated species from Central and South America (WKS,
propagated as populations via seeds); the Groß Lüsewitz
Potato Collections (GLKS; K.J. Dehmer) contain a total of
5,945 accessions and 140 species. 1,161 genebank accessions
were distributed to 177 requestors, 15 coming from
foreign countries (71 accessions shipped).
In 007, 63 AKS and 609 KKS accessions were cultivated
and characterised in the field with ten plants each, and
6 WKS accessions (194 from seedlings, 3 from tubers)
were sexually propagated in the greenhouse. Via in vitro
cultivation, , 7 KKS and 490 AKS accessions are maintained,
while more than 1,000 KKS entries are cryopreserved
at Gatersleben (research group In vitro Storage
and Cryopreservation).
According to the respective ELISA tests, 2,508 in vitro
samples are free of the six most common potato viruses.
The Plant Protection Offices at Hannover and Rostock
tested GLKS potato material for quarantine viruses (330
accs.), quarantine bacteria (349), and PSTVd (586).
Evaluations were carried out for resistance to Globodera
pallida (43 WKS accessions/ 03 genotypes, State Plant
Protection Office Rostock) and Phytophthora infestans
on tubers (91 WKS accessions/449 genotypes; BAZ/ILK).
Tubers of 39 KKS entries were cultivated for the evaluation
of their taste, while tuber material of 4 selected accessions
was propagated for a stability research project.
In the greenhouse, 40 WKS accessions with ten genotypes
each were grown for the production of tubers to be used
48
in potato wart resistance tests and as base material for
protoplast fusions within the KOSY project (BBA, Phytowelt).
Regarding research on duplicate identification within
the KKS, efforts to homologise SSR marker sets for germplasm
characterisations on an international level were
continued.
The Oil Plants and Forage Crops Collections at Malchow
(E. Willner) maintain 14,089 accessions ( ,455 samples of
oil plants, 10,407 forage grasses, 1, 7 forage legumes).
A further increase in the number of accessions resulted
from the transfer of varieties no longer listed on the official
variety list from the Federal Seed Board (Bundessortenamt)
to Malchow (48 cultivars). In 007, a total of 1,330
accessions were cultivated, either for multiplication (738)
and/or characterisation (488) or evaluation (104).
Germination tests were conducted for 1,744 accessions.
According to FAO genebank standards, 54 % of the whole
Malchow collection is stored as an active and base collection
with safety duplicates at IPK Gatersleben (7,673 accessions),
while 81 % of the whole collection is available
for seed requests. A total of 554 samples were provided
to 36 users, whereof 19 seed deliveries with 408 samples
were for foreign countries.
Characterisations were performed on 1,14 grass accessions,
64 samples of rape, mustard or forage kale accessions
for an initial description of their morphological
and phenological traits as well as for the confirmation of
their botanical classification. Field evaluations were carried
out for Lolium perenne (81 accessions); 50 accessions
collected from different European countries and 31 breeding
lines were analysed for trait variability and/or green
matter yield.
For the Genebank management processes that are supported
by the Genebank Information System (GBIS/M),
data were further consolidated (collaboration with M.
Oppermann, H. Knüpffer, U. Lohwasser, research group
Genebank Documentation, research group Resources Genetics
and Reproduction).
The European Central Poa Database (http://poa.ipkgatersleben.de)
was further advanced, containing passport
data of 5, 16 accessions from 38 Poa species (in collaboration
with S. Weise, research group Bioinformatics
and Information Technology). These originate from
56 different countries and are maintained at 1 institutes
in 18 European countries. Progress was made in
the identification of “Originality” and “Primary Holder”
matters, which will allow to avoid unnecessary regenerations
and to improve the sharing of responsibilities.
As a result of a former and joint Lolium project with the
research group Genome Diversity, both E. Willner and
K.J. Dehmer contributed essentially to the development
of a Diversity Study Toolkit (DiSTo) for a combined and
web-based analysis of passport, characterisation, evaluation
and molecular data by the Plant Data Warehouse
group.