reSolution_LNT_No1_en - Leica Microsystems

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BIOLOGY Biological Electron Microscopy Dr. Martin W. Goldberg, Durham University, UK The Electron Microscopy (EM) laboratory in the School of Biological and Biomedical Sciences at Durham University is an integral part of a wider facility spanning a range of advanced imaging tools (laser scanning and spinning disc confocal microscopes, TIRF microscopy and live cell imaging) as well as an ultra high resolution (
Such sample preparation is also amenable to immuno-gold labelling, so that we can use antibodies to locate cargoes and transporters in transit, as well as the proteins that make up this gateway (the NPC) (Fig.4). Contact Dr. Martin W. Goldberg, Durham University, UK m.w.goldberg@durham.ac.uk Instruments related to this sample preparation: Leica EM TP Automated Routine Tissue Processor Leica EM ACE200 Low Vacuum Coater BIOLOGY Fig. 3: High magnifi cation transmission electron micrograph of high pressure frozen, freeze substituted yeast cell, clearly showing both leafl ets of the inner and the outer nuclear membranes, with ribosomes docked on the outer membrane and NPCs at points where the two membranes are joined. Fig. 4: Transmission electron micrograph of high pressure frozen, freeze substituted yeast cell immuno-gold labelled for a NPC protein. Leica EM CPD300 Automated Critical Point Dryer Leica EM ACE600 High Vacuum Coater NANOTECHNOLOGY 7
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Page 11 and 12: References 1. MSDS Nisshin: http://
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Page 17 and 18: Atomic Force Microscopy Study of a
Page 19 and 20: Fig. 2 a,b: Large scale image of a
Page 21 and 22: University of Wollongong Electron M
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reSolution_LNT_No1_en - Leica Microsystems

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