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December 1, 2012 EvolDir 101<br />

Dean<br />

Dr. Dean C. Adams Professor Department of Ecology,<br />

Evolution, and Organismal Biology Department<br />

of Statistics Iowa State University Ames, Iowa 50011<br />

www.public.iastate.edu/ ˜ dcadams/ phone: 515-294-<br />

3834<br />

dcadams@iastate.edu<br />

Software Transformer-4<br />

Dear EvolDir members:<br />

We are pleased to announce the release of the software<br />

T4-2.0.0, which greatly enhances the capabilities of its<br />

precedessor T4-1.3. T4-2.0.0 boosts the analysis of any<br />

genotype matrix in PC, Mac or Linux, and it admits<br />

data coding formats compatible with all the molecular<br />

techniques used in the investigation of biodiversity,<br />

either codominant (allozymes, nuclear microsatellites,<br />

SNP coded with two letters per genotype), or dominant<br />

(chloroplast microsatellites coded with three digits per<br />

haplotype, AFLPs, RFLPs, ISSR or any other molecular<br />

technique that can be binary-coded, or DNA sequence<br />

matrices). DNA sequence, SNP coded with one<br />

letter per genotype, and RAD matrices are considered<br />

dominant by T4 2.0.0, and they can only be imported<br />

to this version of T4 from fasta, mega, nexus, or phylip<br />

files.<br />

T4-2.0.0 is suitable for matrices containing an unlimited<br />

number of alleles per locus, loci, individuals, populations,<br />

or taxa (diploid individuals, in the case of codominant<br />

data). The only limit is the space available in the<br />

hard disk of the user’s computer. Most matrices resident<br />

in T4’s matrix explorer can be transformed easily,<br />

swiftly and simultaneously into the input formats of one<br />

or several of the 38 most commonly used population genetic<br />

software (and for any possible combination of the<br />

populations that each matrix contains).<br />

T4-2.0.0 also allows the users to quickly publish “genetic<br />

diversity digests” in the Demiurge information<br />

system. Such “digests” are peer-reviewed and made up<br />

by a geo-referenced T4 genotype matrix plus any ancillary<br />

information relevant to its interpretation that<br />

the authors see fit to include (see an example of a<br />

published digest in http://www.demiurge-project.org/matrix<br />

digests/14).<br />

To use T4-2.0.0, just register in the Demiurge information<br />

system by clicking on<br />

http://demiurge-project.org/register Examples of all<br />

types of matrices suitable to be imported to T4 are<br />

available in the T4 download page (http://demiurgeproject.org/download<br />

t4). An exhaustive user’s manual<br />

is available upon opening the software (clicking on<br />

“Help/Help contents”)<br />

A note about the T4 v 2.0.0 software and the Demiurge<br />

information system has been submitted to Mol.<br />

Ecol. Res. A short video summarizing the utilities of<br />

this software and the Demiurge system can be seen cliking<br />

on http://vimeo.com/29828406 . Previous versions<br />

of T4 have been downloaded and used by researchers<br />

from 26 countries, and some of the digests published in<br />

Demiurge are already associated with papers in several<br />

impact journals.<br />

T4-2.0.0 and the Demiurge system are deliverables resulting<br />

from several work packages in the project Demiurgo<br />

(MAC/1/C20). These work packages were cofunded<br />

by the Programa de Cooperación Transnacional<br />

Madeira-Açores-Canarias 2007-2013, the Instituto Tecnológico<br />

de Canarias (Gobierno de Canarias), and the<br />

Jardín Botánico Canario ≪Viera y Clavijo≫ -Unidad<br />

Asociada CSIC (Cabildo de Gran Canaria).<br />

On behalf of all the Demiurge team,<br />

Juli<br />

–<br />

Dr. Juli Caujapé Castells<br />

Head Dept. of Molecular Biodiversity & DNA bank<br />

Jardin Botanico Canario “Viera y Clavijo”-Unidad<br />

Asociada CSIC Cabildo de Gran Canaria,<br />

Associate Director of Research UNESCO chair for the<br />

conservation of Plant diversity in Macaronesia and the<br />

West of Africa Cabildo de Gran Canaria.<br />

Ap. de Correos 14 de Tafira Alta 35017 Las Palmas de<br />

Gran Canaria, Spain phone: +34 928219421 ext. 14770<br />

fax: +34 928 219581<br />

The Jardín Botánico Canario “Viera y Clavijo”-<br />

UA CSIC http://www.jardincanario.org/ The Department<br />

of Molecular Biodiversity & DNA Bank<br />

http://www.bioclimac.com/mbdna/ The Demiurge<br />

information system for biodiversity’s genetic diversity<br />

http://www.demiurge-project.org/ http://vimeo.com/29828406<br />

The Island-biodiv project http:/-<br />

/www.island-biodiv.org GreenTank, plataforma informativa<br />

sobre biodiversidad e investigación http://www.greentank.es<br />

The Island Plant Conservation Network<br />

http://www.bgci.org/ourwork/islands/ julicaujape@gmail.com

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