Addendum to PAL User Manual Installation and ... - PAL System

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7.2. Preparation of Test Standard Solution
Addendum to PAL System User Manual
PAL ITEX-2 Option
The test or calibration standard solution as received (concentration
100 µg/mL of each compound) is diluted 1:10 with methanol.
This secondary standard solution is used for the tests as described below.
Ensure that the methanol used for the dilution step is of high purity.
7.2.1. Selective GC Detector, e.g. MSD and ECD
Add 1 µL of the secondary standard solution directly into a 20 mL Headspace
vial and seal the vial immediately. Use a microcap device or a GC syringe to
dose the aliquot of the standard solution.
• Secondary standard solution: Concentration 10 µg/mL
• 1 µL aliquot = 10 ng/ µL
7.2.2. GC Detector FID, TCD or any other Non-selective Device
Add 10 µL of the secondary standard solution directly into a 20 mL
Headspace vial and seal the vial immediately. Use a microcap device
or a GC syringe to dose the aliquot of the standard solution.
• Secondary standard solution: Concentration 10 µg/mL
• 10 µL aliquot = 100 ng/ µL
If the calibration standard is used only for the less sensitive GC Detectors,
such as FID or TCD, then the primary standard with a concentration of
100 µg/mL can be used directly. Add a 1 µL aliquot of the primary standard
solution into a 20 mL headspace vial.
7.3. Instrument Preparation for the ITEX Test
7.3.1. Cycle Composer Macros or ICC- Cycles
The Cycle Composer macros or ICC-cycles used for the ITEX-2 tests are:
• Static Headspace: ITEX2_TestHS
• ITEX enrichment: ITEX2_TestITEX
7.3.2. GC Method Parameters
• GC column: nearly any capillary column can be used to separate the
compounds in the test sample
• GC Injector temperature: 200 to 250°C, split less for 1 min.
• Detector: settings depend on the detector used
• GC oven temperature: 40 0 C, hold 1 minute, 10 0 C/min up to 150 0 C,
hold 2 minutes
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