SodininkyStÄ ir darÅ½ininkyStÄ 28(4)

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specimens is subordinated to pH 7.0. These results allow a supposition that proteins of mitochondria membrane may be involved into formation of IAA-ABP complexes in this organelle. This feature was confirmed by results of the experiments when other methodical approaches have been used for mitochondria sub-fractionation (Hájek et al., 2004). In this case sub-fractionation of mitochondria into membranes and matrix fractions was achieved by their distribution between polar and non-polar phases employing sunflower oil. It is known that under this procedure the membrane structures are not destroyed (Hájek et al., 2004). In such experiments the amount of IAA bound with membrane specimens reaches 5 716 ± 390 cpm and complexes specificity 12 %, i. e. it is higher. Based on these results the following supposition has been formulated: the mitochondria’s ABPs are localized and functioning in membranes. Therefore, to deny or confirm this presumption, the experiments employing solubilized membrane protein specimens are preferable (Jones, 1999; Anisimovienė, Merkys, 2000; Jodinskienė, Anisimovienė, 2007). At initial stages of these experiments the non-ionic detergent Triton X-100, convenient for plasmalemma integral proteins solubilization has been chosen. The optimal concentration of detergent (from 0.5 to 2.0 %) as well as the protein and detergent ratio (from 1 : 1 to 1 : 5) have been selected experimentally by comparison of the main characteristics of IAA-ABP complexes formed in intact mitochondria (Table). Table. Comparison of IAA-ABP complexes characteristics Lentelė. IAR-ASB kompleksų charakteristikų palyginimas Test object Testo objektas Intact mitochondria Sveikos (funkcionuojančios) mitochondrijos Mitochondria membranes specimens Mitochondrijų membranų preparatai Mitochondria membranes protein solubilized with Triton X-100* Mitochondrijų membranų baltymai, solubilizuoti Tritonu X-100* Mitochondria membranes protein solubilized with Digitonin** Mitochondrijų membranų baltymai, solubilizuoti Digitoninu** Amount of specifically bound IAA-ABP complexes 14 C-IAA, cpm × mg -1 protein specificity Specifinis 14 C-IAR IAR-ASB kompleksų prisijungimas specifiškumas (%) imp. × min. -1 × mg -1 baltymo 8 346 ± 435 38–41 5 716 ± 390 12 6 254 ± 361 14–15.5 7 499 ± 482 30–40 * Concentration of Triton X-100 1.75 %, detergent and protein ratio 1 : 3 Tritono X-100 koncentracija – 1,75 %, detergento baltymo santykis 1 : 3 **Concentration of digitonin 1.0 %, detergent and protein ratio 1 : 5 Digitonino koncentracija – 1,0 %, detergento baltymo santykis 1 : 5 156
Although under high detergent Triton X-100 concentration and high protein : detergent ratio implication we did not succeed to achieve the expected results. Apparently, we did not achieve full solubilization of these membrane integral proteins (Jones, 1999; Anisimovienė, et al., 2000). Because, when for solubilization of these proteins detergent digitonin (Eubel et al., 2003) 1 % concentration, specific for mitochondria membranes solubilization was applied, the same or almost the same amount of IAA specifically bound with ABP unit and specificity of formed complexes have been determined (Table). Discussion. During the last two decades auxin binding sites localization on plasmalemma and cytosol, IAA-protein interaction peculiarities as well as the role of these complexes in auxin hormonal system action have been widely investigated (Darginavičienė, 1992; Merkys et al., 1998; Anisimovienė et al., 2004; Zazimalova, Napier, 2003). Earlier experiments (Merkys et al., 2003; Anisimovienė et al., 2004; Mockevičiūtė et al., 2006; Jodinskienė, Anisimovienė, 2007) revealed at least seven different binding sites functioning in dicot (kidney bean) plant cells. Two of them are functioning in plasmalemma (optimal pH for IAA-ABP complexes formation 5.5 and 7.5). Two IAA-ABPs complexes are formed cytosol. The optimal pH for both low affinity (molecular mass > 67 kDa) and high affinity (≈ 30 kDa) binding sites is 7.8. Two IAA-ABP complexes (optimal pH 5.5 and 7.5) formed in kidney bean leaves cells chloroplast were also ascertained (Anisimovienė et al., 2004). Based on experimental data the presumption that IAA binding sites, which formed IAA-ABP complexes at pH optimal 5.5 are localized and functioning in stroma, while other formed in chloroplast (optimum pH for IAA-ABP complexes formation at 7.5) may be related to the proteins localized in membranous structures (Mockevičiūtė et al., 2006) has been formulated. One binding site is localized in mitochondria (Anisimovienė et al., 2004). Presented results support the possibility of ABPs functioning in membranes of this organelle. These results may be considered as to be in agreement with the date obtained by other investigators, in which the possibility of IAA synthesis in mitochondria from amino acid tryptophane has been determined. The occurrence of natural IAA in both plant cell organelles – chloroplast and mitochondria – in sunflower, barley leaves using methods of biochemical analysis and in leaf cells of peach using immunocitochemical analysis (Wighman, Fregeau, 1983; Ohmyia, Hyashi, 1992) has been proved. They may be also be considered in agreement with data on the possibility of bacteria – according to endosymbiotic theory progenitors of these organelles in plant cell (Gay, 1999) – to synthesize high amounts of IAA and segregate this compound out of cell (Libbert et al., 1969; Merkys et al., 1974; Sergeeva et al., 2002; Karadeniz et al., 2006). In spite of the discovery of the possible of IAA-ABP complexes formation in intact and functioning mitochondria (Merkys et al., 2004; Anisimovenė et al., 2004) and localization of IAA binding sites in their membranes of it, the consideration on the possible role of these complexes formed in this organelle in IAA system action or signaling is problematic because these questions are still no investigated. While the characteristics of IAA-ABP complexes formed in mitochondria of kidney bean cells differ from ABP-IAA-receptor complex, having the carboxyl group binding domain /-His-Arg-His-/ and functioning in plasmalemma, it is possible to 157
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Lietuvos sodininkystĖs ir darŽini
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LIETUVOS SODININKYSTĖS IR DARŽINI
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1 lentelė. Sodo konstrukcijų įta
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3 lentelė. Sodo konstrukcijų įta
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4. Pagal kamieno skerspjūvio plot
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Tais metais didžiausi spalvos pasi
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1 pav. Tirpių sausųjų medžiagų
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Literatūra 1. Bauman H. 1998. Decr
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SCIENTIFIC WORKS OF THE LITHUANIAN
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May. Flight of the second generatio
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Then it reached 0.6 % in the second
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7. Charmillot P. J., Pasquier D., S
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ir -8 °C temperatūrose. Išimtos
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3 pav. Žiedinių pumpurų pažeidi
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žiedinių pumpurų. Kitos tirtos v
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elektroforezės metodu (Laemmli, 19
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2 pav. Termostabilių baltymų kiek
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Padėka. Tyrimus rėmė Lietuvos va
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27. Wisniewski M. E., Arora R. 2000
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Tyrimo objektas, metodai ir sąlygo
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4 lentelė. Kaulavaisių sodinamosi
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10. Wycior S., Kiczorowski P., Wojc
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Dažnai braškių auginimo technolo
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Po derliaus nuėmimo labiausiai rud
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išvaizda lenkė ‘Figaro’, ‘D
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tirtų veislių uogos buvo tvirtesn
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SODININKYSTĖ IR DARŽININKYSTĖ. S
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Heiberg ir kt., 2002; Danek, 2004).
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Veislė Cultivar ‘Novokitajevskaj
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‘Novokitajevskaja’, ‘Obilnaja
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Ištvermingiausios žiemą yra tų
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4. Ferticare 14-11-25, three times
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Table 2. Average yield per plant (g
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Table 4. Share of cull berries in t
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7. Kivijärvi P. 2002. Cultivation
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1 pav. ‘Elkat’ veislės braški
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tiek senuose (2 B ir D pav.), tiek
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2. Brown C. S., Schuerger A. C., Sa
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T o t a l a e r o b i c m i c r o o
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Data presented in Fig. 3 reveal tha
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Discussion. Data obtained in our pr
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8. Luksiene Z., Peciulyte D., Jurko
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Karklelienė, 2006 b). Veislė yra
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et lyginant su veisle ‘Perfekcija
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tumų negauta. Lyginant vidutinio a
Page 107 and 108: SCIENTIFIC WORKS OF THE LITHUANIAN
Page 109 and 110: C o s t s o f t r a c t o r a n d f
Page 111 and 112: The costs structure (Fig., Table 2)
Page 113 and 114: Table 2 continued 2 lentelės tęsi
Page 115 and 116: production of vegetables, particula
Page 117 and 118: LIETUVOS SODININKYSTĖS IR DARŽINI
Page 119 and 120: ‘Babtų didžiųjų’ veislės s
Page 121 and 122: nei daugiamečiai mėnesio rodiklia
Page 123 and 124: Suminis svogūnų ropelių derlius
Page 125 and 126: Mažiausi cukrų kiekiai buvo kalci
Page 127 and 128: Literatūra 1. Adamicki F. 2006. Ef
Page 129: yield was obtained fertilizing addi
Page 132 and 133: fotosintezės intensyvumą dėl did
Page 134 and 135: temperatūra, kaip jau buvo minėta
Page 136 and 137: 2 pav. Karotinoidų kiekis valgomoj
Page 138 and 139: Literatūra 1. Baxter R., Ashenden
Page 143 and 144: skyrėsi. Trečio matavimo metu (pr
Page 145 and 146: Derėjimo pradžioje didžiausią c
Page 147 and 148: 4 pav. Agurkų, augintų skirtingo
Page 149 and 150: 6. Espinola H. N. R., Andriolo J. L
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Page 153 and 154: 10 mM TRIS-HCl, pH 8.0, 10 mM TRIS-
Page 155: In order to ascertain if the destru
Page 159 and 160: 3. Anisimovienė N., Mockevičiūt
Page 161: 35. Zhang X. P., Glaser E. 2002. In
Page 164 and 165: jos tirpalai slopino fotosintezės
Page 166 and 167: 1 pav. Lapų vystymosi tarpsnio sė
Page 168 and 169: 4 pav. Šoninių ūglių formavimos
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Page 172 and 173: 19. Papazoglou E. G., Karantounias
Page 174 and 175: augalų mityba. Patirtis rodo, kad
Page 176 and 177: Visai netręšti augalai buvo blyš
Page 178 and 179: Literatūra 1. Bartaševičienė B.
Page 183 and 184: iki 230 ± 22,4 g - vidutiniškai 4
Page 185 and 186: Ilgiausiai (2-3 paras) džiūsta st
Page 187: 14. Wills R. B. H., Shohet D. 2008.
Page 190 and 191: ląstelių atsinaujinimui (Singer i
Page 192 and 193: Poveikio metu augusių kukurūzų a
Page 194 and 195: Aptarimas. Vis dažniau susiduriama
Page 196 and 197: 13. Mohammadkhani N., Heidari R. 20
Page 201 and 202: skirtinga. Vieni teigia, kad bulvi
Page 203 and 204: trąšomis, iš esmės padidėjo bu
Page 205 and 206: 4 lentelė. Fosforitmilčių ir Pat
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edokso potencialo reikšmė, palygi
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application of potassium fertilizer
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- Rezultatai Trumpai išdėstomi ty
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Straipsnis knygoje: 1. Streif J. 19
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- Object, methods and conditions -
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Article in book: 1. Streif J. 1996.
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L. Buskienė Penkiolikos aviečių
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J. Pekarskas, D. Šileikienė Fosfo
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SodininkyStÄ ir darÅ½ininkyStÄ 28(4)