OÄ¼ehnoviÄs D., atbildÄ«gais par izd. / Resp. Ed ... - DU conference

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DAUGAVPILS UNIVERSITĀTES 53. STARPTAUTISKĀS ZINĀTNISKĀS KONFERENCES TĒZES ABSTRACTS OF THE 53 rd INTERNATIONAL SCIENTIFIC CONFERENCE OF DAUGAVPILS UNIVERSITY klimatiskajiem apstākļiem. Savukārt NBD kolekcijā ievāktais Tālo Austrumu pīlādţu sugu sēklu materiāls nosūtīts uz 35 pasaules valstīm. Norādīti konkrēti botāniskie dārzi un tiem pielīdzinātas institūcijas, no kurām saľemts un kurām nosūtīts sēklu materiāls. Īpaši vērtīgas ir genofonda vienības, kuras izaudzētas no dabā ievākta sēklu materiāla. Baiba Tikuma baibacirava@inbox.lv DZĒRVEŅU APPUTEKSNĒTĀJU AKTIVITĀTES ATKARĪBA NO TO IZVIETOJUMA ATTĀLUMA Latvijā lielogu dzērvenēm (Vaccinium macrocarpon Ait.) ir labvēlīgi augšanas apstākļi, plašas augsto sūnu purvu platības. Šobrīd lielogu dzērvenes aizľem 120 ha lielu platību, bet perspektīvā plānots tās palielināt līdz 300 ha. Viena no aktuālajām problēmām Dzērveľu audzēšanā Latvijā ir augstāku raţu un kvalitatīvu ogu ieguve, ko iespējams risināt piesaistot papildus apputeksnētājus – bites un kamenes. Veikts pētījums <strong>par</strong> bišu un kameľu izvietojumu stādījumu platībās un dz;erveľu apputeksnēšanās efektivitāti. Darbā tika uzskaitīts apputeksnētāju skaits, lidojuma attālums līdz stropam, ziedēšanas intensitāte, konkurentaugi. Apputeksnēšanas efektivitāte tika vērtēta pēc 100 ogu masas, raţas no 1m 2 , vertikālo dzinumu skaita u.c. faktoriem.
DAUGAVPILS UNIVERSITĀTES 53. STARPTAUTISKĀS ZINĀTNISKĀS KONFERENCES TĒZES ABSTRACTS OF THE 53 rd INTERNATIONAL SCIENTIFIC CONFERENCE OF DAUGAVPILS UNIVERSITY ĢENĒTIKA UN BIOTEHNOLOĢIJAS / GENETICS AND BIOTECHNOLOGIES Lita Lapiľa, Dace Grauda, Isaak Rashal lita555@inbox.lv USING RETROTRANSPOSON-BASED MOLECULAR MARKERS FOR ANALYSIS OF GENETIC VARIABILITY IN LATVIAN POPULATIONS OF ALFALFA Key words: Medicago sativa, genetic diversity, retrotransposons Retrotransposons belongs to the mobile genetic elements and are ubiquitous in the genomes of many eukaryotic organisms. There are several marker systems based on the analysis of distribution of different retrotransposons in genome. The limiting factor of most of them is a necessity of development for each plant species the own molecular marker systems depending on specific retrotransposon sequences. Till now, they are not available for all crops of the interest. Recently (2010) Kalendar and Schulman developed a new universal method for DNA fingerprinting which is based on location of specific inter primer binding sites (iPBS) of the long terminal repeat (LTR) retrotransposons. Several PCR primers were suggested for use in different plant species. The goal of our work was to reveal among them most effective ones for analysis of alfalfa accessions. Sixteen specific PCR primers were tested to find out primers showing highest level of polymorphism in samples involved in the investigation. Selected primers were applied to Latvian alfalfa genetic resources consisting from breeding varieties and semi-wild populations. Andra Mikelsone, Dace Grauda, Aija Auzina, Isaak Rashal 1 andra_mi@inbox.lv USING IN VITRO METHODS FOR FLAX BREEDING INTENSIFICATION Key words: flax, breeding, somatic callus culture, anther culture Flax breeding is a long and complicated process based on hybridization and following selection of the best plants. Because possible cross-pollination, development of genetically stable homozygous lines could last more than 10 years. For the more rapid creating of initial material for flax breeding we are using two in vitro methods: somatic callus cultures for inducing additional genetic variation, and anther cultures for producing doubled haploid lines. The aim of the study was improving of conditions of somatic and androgenetic callus growing and obtaining plantsregenerants. Effects of genotype, explants, and different biological active substances were investigated. For both types of callus cultures optimal composition of medium was established for all crucial in vitro cultivation stages, including callus induction, organogenesis, plant regeneration. Elaborated protocols are used for creation of initial breeding material for Latvian flax breeding.
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