medicină veterinară - Ion Ionescu de la Brad

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Universitatea de Știinţe Agricole și Medicină Veterinară research has been to appreciate the preclinical antineoplastic effectiveness of the glucanic biopreparation. We studied the possibility to increase their antitumoral potential by the experimental modulation of the therapeutical doses, and we also compared its efficiency with the impact of standard cytostatics upon the tumor development process. MATERIALS AND METHODS The original bioactive agent – used in the treatment of various experimental tumoral systems of solid type for the preclinical quantitative pharmacological evaluation of its oncostatic action – namely EGlClp, is an extract of glucanic nature (17), which was specifically obtained from the cultivation medium supernatants of the Claviceps purpurea fungus submerged cultures, by ethanolic precipitation, centrifugation, sediment resuspendation in distilled water, dialization, ethanolic reprecipitation and final centrifugation. The standard cytostatics, included in the reference experimental antitumoral therapy, were: methotrexate, cyclophosphamide, melphalan and 5-fluorouracil. The in vivo tests were performed in appropriate experimental models, using white, female Wistar rats – pure line – of 125-150 g b.w, with either Guérin T-8 lymphotropic epithelioma or Walker 256 carcinosarcoma. The animals were housed in individual cages having free access to water and standard food in a normal light/dark cycle and at 22 0 C ambient temperature. The tumoral transplant was performed by the subcutaneous injecting of 0.2 ml of cell suspension, obtained by homogenizing cancerous tissue in saline solution containing streptomycin and penicillin (27). The experimental antitumoral treatment was initiated 24 hours after the tumoral transplant and was continued for 16 and 19 days, respectively, in the case of Guérin tumor and Walker 256 line, respectively. It consisted in daily intraperitoneal (i.p.) administration of the bioactive agent in various doses. Thus, the injected doses of natural glucanic biopreparation have been either higher or lower than the dose which conditioned the expression of its antitumoral action (20 mg/kg. body weight = b.w.). The doses of the standard oncochemotherapeutics were established in relation to that used in clinical antineoplastic therapy. The values of the therapeutical doses, expressed in mg/kg. b. w., are presented in the tables that also include the results for each of the experimental models. An equal volume of saline solution was injected to the control animals. The oncostatic impact was assessed by determining the rate of mean tumor regression (%M.T.R.), as well as by calculating the T/C ratio (where T = M.T.W. for the treated groups and C = M.T.W. for the control group) and the statistic significance by means of Student's “t” test (3, 11, 12, 14, 19, 33, 40). The evaluation of the anticancerous effect was performed by comparing the mean tumor weight (M.T.W.) of the treated and, respectively, control rats after slaughtering. The appreciations of the antitumoral therapeutic effectiveness of the EGlClp also required the comparative analysis of the evaluation indices values we obtained with those set by the selection criteria of antineoplastic substances established in the preclinical screening programs of the Microbiology and Experimental Therapy Institute of Germany (14, 19) and of the National Cancer Institute of the USA (12, 19, 40) for this phase of the preclinical trial. RESULTS The interference of daily antitumoral therapy, performed by administration of EGlClp different doses, with the development process of Guérin T-8 lymphotropic epithelioma, can be followed from Table 1. 342
Lucrări Științifice – vol. 51 seria Medicină Veterinară Table 1. Experimental oncochemotherapeutic potential of various doses of EGlClp biopreparation (mg/kg b.w./day) upon Guérin T-8 lymphotropic epithelioma or Walker 256 carcinosarcoma. Figures in brackets indicate the number of experimental animals. Group / Guérin T-8 lymphotropic epithelioma Treatament % T/C M.T.W.(g) M.T.R. value p Control 19.4 ± 3.1 (15) – – – 10 mg/ kg b.w. 15.3 ± 2.2 (10) 21.1 0.79 NS 20 mg/ kg b.w. 10.8 ± 1.3 (10) 44.3 0.56
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ISSN 1454-7406 UNIVERSITATEA DE ŞT
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CUPRINS: 1. ABDELFATTAH Nour EFFECT
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28. OLARIU-JURCA I., COMAN M., LAZ
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55. BRĂSLAŞU M.C., BRĂSLAŞU M.C
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85. GRECU Mariana, HRIŢCU Luminiţ
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118. ROŞCA P., DRUGOCIU D., RUNCEA
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146. ARSENE Marinela OBSERVATII PRI
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174. FIŢ N., RĂPUNTEAN Gh., ŞUTE
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201. POPESCU Raluca, HORHOGEA Crist
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231. VOICU Elena, CARP-CĂRARE M.,
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Universitatea de Știinţe Agricole
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grame Universitatea de Știinţe Ag
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(log₁₀) Celule somatice/ml lapt
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IF (mN/mg tesut umed) Forta (mgF) U
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indice de forta la administrarea de
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Y = a * x + b Universitatea de Ști
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January February March April May Ju
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Martor Ziua 0 Ziua 7 Ziua 14 Ziua 2
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G (%) Universitatea de Știinţe Ag
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INDICELE DE INSAMANTARE SP (ZILE) U
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Index după autori: ABDELFATTAH Nou
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H HAGIU N., 275, 279 HERMAN V., 310
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SIMEANU D., 927, 933 SIMION Violeta
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medicină veterinară - Ion Ionescu de la Brad

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