CamSep 2 S

DETECTION OF EDCs AND RELATED COMPOUNDS FROM TREATED
SEWAGE WATER AND SELECTED SURFACE WATER ECOSYSTEMS via
Micro-TLC USING PHOSPHOMOLYBDIC ACID STAINING AND
FLUORESCENCE VISUALIZATION
Paweł K. ZARZYCKI * , Elżbieta WŁODARCZYK * , Michał J. BARAN * ,
Magdalena M. ŚLĄCZKA * , Filip B. HARASIMIUK * , Paweł PISZCZ ** , Bronisław
K. GŁÓD **
* Section of Toxicology and Bioanalytics, Koszalin University of Technology, Sniadeckich 2, 75-453
Koszalin, Poland; E-mail address: pkzarz@wp.pl
** Department of Analytical Chemistry, Institute of Chemistry, Faculty of Science, University of
Podlasie, 3 Maja 54, 08–110 Siedlce, Poland
This study is a continuation of our previous research focusing on development of micro-TLC
methodology under temperature-controlled conditions [1,2]. The main goal of present paper is to
demonstrate the separation and detection capability of micro-TLC technique involving simple
analytical protocols without multi-steps sample pre-purification. One of the advantages of planar
chromatography over its column counterpart is that each TLC run can be performed using nonpreviously-used
stationary phase. Therefore, it is possible to fractionate or separate complex
samples characterized by heavy biological matrix loading. In present studies components of interest
were isolated from environmental samples characterized by wide range of organic compounds
loading. Low-molecular-mass compounds from lake water, untreated and treated sewage waters
were concentrated using optimized solid-phase extraction (SPE). Our investigations has revealed
that specific bands patterns for samples derived from surface water of the Middle Pomerania in
northern part of Poland, can be easily observed on obtained micro-TLC chromatograms (Fig. 1 A-
D). Described methodology can be applied for fast fractionation or screening of target substances
and fingerprinting of complex mixtures, which are present in raw biological or environmental
samples.
Fig. 1. Micro-TLC plate spotted with SPE extracts derived from environmental samples including
surface and sewage waters. Spots visualization: A - direct visible light, B - UV light (254 nm), C -
UV light (366 nm), D - visible light after PMA derivatization.
References
[1] P.K. Zarzycki; “Simple horizontal chamber for thermostated micro-thin-layer chromatography”; J. Chromatogr. A.
1187 (2008) 250–259; DOI:10.1016/j.chroma.2008.02.013
[2] P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, E. Włodarczyk, M.J. Baran, T. Heese, B.K. Głód, “Fast separation
and detection of main components in complex raw biological materials using temperature-controlled micro-TLC”,
Pomiary Automatyka Kontrola, 56 (4) (2010) 360-364.
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