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OPERATOR'S MANUAL BIOPROtect II JR Vent to ... - Baker Company

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OPERATOR’S <strong>MANUAL</strong><br />

<strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong><br />

<strong>Vent</strong> <strong>to</strong> Room / <strong>Vent</strong> <strong>to</strong> C.E.C /<br />

<strong>Vent</strong> <strong>to</strong> H.E.C<br />

Bio-Containment Enclosure<br />

This manual includes information for installation, operation, maintenance and spare parts.<br />

We recommend that it be kept near the cabinet for ready reference.<br />

November, 2006<br />

Rev C<br />

THE BAKER COMPANY<br />

161 Gatehouse Rd., Sanford, Maine 04073 USA ⋅ (207) 324-8773 ⋅ 1-800-992-2537 ⋅ FAX (207) 324-2632<br />

Internet address: http// www.bakerco.com<br />

“Creating Immaculate Atmospheres”<br />

http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc


The <strong>Baker</strong> <strong>Company</strong><br />

INTRODUCTION AND WELCOME<br />

It is a pleasure <strong>to</strong> welcome you <strong>to</strong> the growing number of cus<strong>to</strong>mers who own and operate <strong>Baker</strong><br />

biological safety cabinets. As the inven<strong>to</strong>rs of the laminar flow biological safety cabinet and the leaders in<br />

the field, <strong>Baker</strong> people take special pride in providing a cabinet that is designed for maximum performance.<br />

Your new <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet includes many unique features which are included <strong>to</strong> give you<br />

superior performance, simpler maintenance and lower life cycle cost. Your <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet is<br />

designed for both safety and value.<br />

In addition <strong>to</strong> the high quality you expect from all <strong>Baker</strong> equipment, this model has been ergonomically<br />

designed <strong>to</strong> provide the lab user with many exciting design features. The revolutionary ergonomic design<br />

will help prevent repetitive motion injury, reduce fatigue and lab accidents and enhance productivity.<br />

You will find your <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet suitable for use not only for research and clinical diagnostic<br />

work involving tissue culturing of possibly infectious samples, but also for I.V. drug preparations and other<br />

pharmaceuticals that could have adverse health effects on opera<strong>to</strong>rs and other techniques requiring a<br />

contamination-free atmosphere.<br />

Please note that all open-front containment cabinets, including this one, are for use with low <strong>to</strong> moderate<br />

risk agents only. Open-front cabinets do not provide absolute protection for the user. The adequacy of a<br />

cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other<br />

qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your<br />

cabinet at your own risk.<br />

We recommend that this manual, along with fac<strong>to</strong>ry test report, be kept near the cabinet for convenient<br />

reference by opera<strong>to</strong>rs and qualified maintenance personnel. If you have any questions about the use or<br />

care of your new <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet, please do not hesitate <strong>to</strong> contact our Cus<strong>to</strong>mer Service<br />

Department at 800-992-2537 for assistance or e-mail us at bakerco@bakerco.com.<br />

Sincerely,<br />

Dennis Eagleson<br />

President, CEO<br />

The <strong>Baker</strong> <strong>Company</strong>, Inc.<br />

P.O. Drawer E, Sanford, Maine 04073 (207) 324-8773 1-800-992-2537 FAX (207) 324-3869<br />

Visit our website at www.bakerco.com<br />

http://solid/prereleased/prereleased/STAN. PRODUCT/313/DOCS/313D000.doc


Table of Contents<br />

INTRODUCTION AND WELCOME ................................................................................................ i<br />

Table of Contents................................................................................................................................ii<br />

I - FUNCTION AND DESCRIPTION OF THE <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> CABINET .............................. 1<br />

Airflow Inside the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> Cabinet ............................................................................... 1<br />

Access <strong>to</strong> the Work Area................................................................................................................ 2<br />

Design Details ................................................................................................................................ 3<br />

Performance assurance.............................................................................................................. 3<br />

All-metal plenums..................................................................................................................... 3<br />

Tested HEPA filters .................................................................................................................. 3<br />

Work area lighting .................................................................................................................... 3<br />

Iris Port...................................................................................................................................... 3<br />

Optional Height Adjustable Cart .................................................................................................... 3<br />

Specifications ................................................................................................................................. 4<br />

Dimensions: .............................................................................................................................. 4<br />

Weights ..................................................................................................................................... 4<br />

Utilities: .......................................................................................................................................... 4<br />

Mechanical................................................................................................................................ 4<br />

Electrical ................................................................................................................................... 4<br />

Environmental Conditions.............................................................................................................. 5<br />

Symbols and Terminology ............................................................................................................. 5<br />

<strong>II</strong> - PREPARING THE <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> CABINET FOR USE ..................................................... 6<br />

The Uses of the <strong>BIOPROtect</strong> <strong>II</strong> Jr.................................................................................................. 6<br />

Location Within the Labora<strong>to</strong>ry ..................................................................................................... 6<br />

Opera<strong>to</strong>r Controls: .......................................................................................................................... 7<br />

1.Blower On / Off Switch – ......................................................................................................7<br />

2.Light On / Off Switch – ......................................................................................................... 7<br />

3.Mute switch - ......................................................................................................................... 7<br />

4 Left Side & Right Side Outlet On / Off –............................................................................... 7<br />

Ground Fault Circuit Interrupter .................................................................................................... 8<br />

Air Pressure Gage......................................................................... Error! Bookmark not defined.<br />

Air Pressure Alarm ......................................................................................................................... 8<br />

Door Operations ............................................................................................................................. 9<br />

To open the viewscreen: ........................................................................................................... 9<br />

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To open the door: ...................................................................................................................... 9<br />

Removing the cart........................................................................................................................... 9<br />

Set-up Procedure .......................................................................................................................... 10<br />

Start-up procedure ........................................................................................................................ 11<br />

Reacting <strong>to</strong> Spills.......................................................................................................................... 12<br />

Decontamination........................................................................................................................... 13<br />

About the HEPA Filters................................................................................................................ 15<br />

IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES ............................................... 16<br />

Filter Media and Seal Leak Tests ................................................................................................. 16<br />

Procedure for filter leak tests........................................................................................................ 17<br />

The Airflow Balance .................................................................................................................... 18<br />

Airflow Smoke Pattern Test ......................................................................................................... 19<br />

Grounding Continuity Test........................................................................................................... 19<br />

Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Moni<strong>to</strong>r)............ 20<br />

Initial Set Point Settings.......................................................................................................... 20<br />

Low Flow Alarm Setting ........................................................................................................ 20<br />

Check the Calibration ............................................................................................................. 20<br />

Modus Programming Instructions ................................................................................................ 21<br />

1.) Programming the Alarm Set points ................................................................................... 21<br />

2.) Programming the Relay Dead band................................................................................... 21<br />

3.) Returning <strong>to</strong> Normal Operation......................................................................................... 21<br />

Maintenance Notes ....................................................................................................................... 22<br />

Replacing the HEPA Filter........................................................................................................... 22<br />

Changing the Exhaust Filter.................................................................................................... 22<br />

Changing the Supply Filter ..................................................................................................... 23<br />

V. - TROUBLE SHOOTING .......................................................................................................... 24<br />

REPLACEMENT PARTS LIST .................................................................................................. 26<br />

VI. APPENDIX ................................................................................................................................ 27<br />

Modus Installation and Operation Handbook............................................................................... 28<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 1 ....................................................................... 42<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 2 ....................................................................... 43<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 3 ....................................................................... 44<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 1 ..................................... 45<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 2 ..................................... 46<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 3 ..................................... 47<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 1.......................................... 48<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 2.......................................... 49<br />

BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 3.......................................... 50<br />

Wiring Diagram-Standard <strong>BIOPROtect</strong> <strong>JR</strong>.................................................................................. 51<br />

Wiring Diagram – Master <strong>BIOPROtect</strong> <strong>JR</strong> .................................................................................. 53<br />

WARRANTY ................................................................................................................................... 54<br />

TEST REPORT…………………………………………………………………………ATTACHED<br />

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I - FUNCTION AND DESCRIPTION OF THE <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> CABINET<br />

The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> is a Class <strong>II</strong> Bio-Containment Enclosure designed <strong>to</strong> house labora<strong>to</strong>ry robotics or<br />

large apparatus that would not ordinarily fit in a standard Class <strong>II</strong> Biological Safety Cabinet (BSC). Some<br />

important features of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> include, vertical laminar airflow, a front access opening, as well<br />

as supply & exhaust HEPA filters. The unit is designed <strong>to</strong> protect not only the environment and the people<br />

using the cabinet, but also the product within from airborne contaminants. The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> can be<br />

configured <strong>to</strong> vent three different ways. <strong>Vent</strong> <strong>to</strong> the room, <strong>Vent</strong> <strong>to</strong> a Canopy Exhaust Connection (C.E.C.)<br />

via a house exhaust system or, <strong>Vent</strong> <strong>to</strong> a Hard Exhaust Connection (H.E.C.) via a house exhaust system.<br />

Although your unit has passed a microbiological test, a modification of NSF/ANSI standard-49-2002e, the<br />

<strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> is not currently listed with the NSF.<br />

Airflow Inside the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> Cabinet<br />

Figure 1 shows the name and location of component parts and the typical airflow pattern of the<br />

<strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong><br />

The stainless steel metal diffuser (1) just below the supply HEPA (High Efficiency Particulate Air) filter<br />

assures that the work area is bathed in HEPA filtered air. The supply air stream splits so that some is<br />

directed in<strong>to</strong> the front grille (2) and the balance is pulled in<strong>to</strong> the perforated grilles on the rear wall (3). On a<br />

flat table <strong>to</strong>p, in the absence of any obstacles, the air should split roughly in the center of the table.<br />

Because the cabinet must take in air <strong>to</strong> replace the air which is expelled through the exhaust filter, the<br />

same quantity of room air enters the cabinet through the eight inch front access opening. The flow of the<br />

intake air provides the personnel protection (containment) aspect of the cabinet.<br />

The front grille of the cabinet is integral <strong>to</strong> the large hinged door. This door, as well as the side and rear<br />

walls, are maintained under a negative pressure. Air is drawn from the front intake grille, in<strong>to</strong> the hollow<br />

door, and in<strong>to</strong> the right side wall. In this manner the outside air that is drawn in<strong>to</strong> the cabinet as intake is not<br />

allowed <strong>to</strong> penetrate the table <strong>to</strong>p area of the cabinet.<br />

Any and all contaminated air that is captured in the cabinet’s negative pressure plenums (front door, side<br />

walls, rear walls or upper plenum) is pulled in<strong>to</strong> the cabinet’s blower (4). The blower discharges in<strong>to</strong> a steel<br />

box that is a common plenum for the supply and exhaust HEPA filters (5). In this manner, all the air that is<br />

handled by the cabinet is HEPA filtered. The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> will exhaust a quantity of air (6) equal <strong>to</strong><br />

that received at the front intake grille. If a leak should occur in a contaminated pressure plenum the negative<br />

pressure will create suction and pull air in, not allowing it <strong>to</strong> escape in<strong>to</strong> the labora<strong>to</strong>ry. If there is a leak<br />

from the positive pressure plenum, the surrounding negative pressure area will recapture the contaminated air<br />

and recirculate it through a HEPA filter.<br />

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Access <strong>to</strong> the Work Area<br />

Figure 1 Typical section with airflow visualization arrows<br />

The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> is provided with three modes of access <strong>to</strong> the work area; via the large hinged door<br />

that can be opened <strong>to</strong> access the entire work area, the hinged viewscreen (providing access <strong>to</strong> the work<br />

surface) or from the 8” x 42” access (sash) opening between the bot<strong>to</strong>m of the closed viewscreen and the air<br />

intake grate. The primary means of access must be through the 8” sash opening. This is the only means of<br />

access that will ensure the user protection from contaminants within the enclosure.<br />

The opera<strong>to</strong>r is only protected from airborne particulate when the large front door and the hinged viewscreen<br />

is closed and secured <strong>to</strong> provide an 8” x 42” access (sash) opening<br />

For easy set-up of equipment in the work area, the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> has one large door that exposes the<br />

entire work area from floor <strong>to</strong> ceiling <strong>to</strong> an area of 51.5” wide x 68” tall.<br />

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Design Details<br />

Performance assurance<br />

Meticulous care in manufacturing is followed by more than 14 separate performance tests prior <strong>to</strong><br />

shipment of your cabinet. In addition, a complete fac<strong>to</strong>ry test report on the operation of your unit is<br />

included at the rear of this manual.<br />

All-metal plenums<br />

The work area surfaces and plenums of your <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> have been constructed entirely of<br />

metal in order <strong>to</strong> provide strength, durability, air-tightness and resistance <strong>to</strong> deterioration. External<br />

construction is of 16-gauge cold-rolled steel, protected by a smooth white baked enamel finish.<br />

Tested HEPA filters<br />

Supply and exhaust HEPA filters in the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> unit have been found <strong>to</strong> be 99.99 percent<br />

effective on particles of 0.3 micron size. Each filter is scan-tested <strong>to</strong> assure leak-free installation.<br />

Work area lighting<br />

The fluorescent lights are externally mounted within the light canopy <strong>to</strong> minimize heat buildup in the<br />

work area. The unit uses two common F32T8 fluorescent bulbs powered by an efficient and reliable<br />

electronic ballast.<br />

Cable Port<br />

A 2.75” I.D. cable port is provided in each side wall <strong>to</strong> accommodate communications ports from<br />

process equipment without compromising performance.<br />

Optional Height Adjustable Cart<br />

The all stainless steel cart, which is optional on the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> was designed <strong>to</strong> accommodate<br />

process equipment weighing no more than 500 Lbs. It’s work surface dimensions are 44” Wide x 34” Deep.<br />

The height of the worksurface can be adjusted by the opera<strong>to</strong>r from 27” <strong>to</strong> 39” by using the manual crank<br />

provided at the front of the cart. For ideal ergonomic performance the cart should be adjusted so that the<br />

work table of the equipment is at the same level as the front intake grille of door on the front of the cabinet.<br />

The cart is provided with swivel casters, all of which are locking. To retract the cart from the work area,<br />

tip the foot operated locking devices at each front caster <strong>to</strong> a horizontal position then pull the cart out of the<br />

work area. To return the cart <strong>to</strong> the cabinet push the cart back in<strong>to</strong> the work area and lock the front casters<br />

by pushing down on the foot operated locking devices. Make certain that the crank handle for the lift system<br />

is facing the front of the cabinet and is retracted under the work table before closing the door.<br />

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Specifications<br />

Dimensions:<br />

Outside: 66” W x 64.44” D x 106.41”H<br />

Usable work area: 51”W x 36.5”D x 75”H (under diffuser)<br />

Cart worksurface: 44”W x 34” D x (27”- 37”) H (height adjusted by opera<strong>to</strong>r).<br />

Weights<br />

Utilities:<br />

Mechanical<br />

Unit weight: The weight of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet is approximately 1200 lbs.<br />

Cart weight: The weight of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cart is approximately 250 lbs.<br />

Maximum worksurface capacity: 500 Lbs. uniform load.<br />

Point loads not <strong>to</strong> exceed 125 Lbs. each<br />

Four (4) 3/8” NPT female connections provided for plumbing; all are pre-plumbed <strong>to</strong> the <strong>to</strong>p of the<br />

unit using 3/8” copper tubing. No service fixtures or valves are provided. User must supply fixtures <strong>to</strong><br />

mate with 3/8” NPT female connection on side walls (see general arrangement drawing for details).<br />

Electrical<br />

!!! This cabinet is powered from more than one power source. The cabinet is not electrically safe unless<br />

all power sources are disconnected. Proper Lockout/Tag out procedures should be used !!!<br />

All electrical wiring should comply with the National Electric Code and any applicable Local Electrical<br />

Codes at the site of installation.<br />

The standard <strong>BIOPROtect</strong> <strong>II</strong> Jr has three junction boxes located on the front of the <strong>to</strong>p deck. The<br />

following services need <strong>to</strong> be connected <strong>to</strong> the junction boxes.<br />

One 120Volt, 20Amp, 60 Hz, Single phase connection <strong>to</strong> power the blower. This circuit should be wired<br />

using 12 AWG wire and provided with a 20 Amp circuit breaker that will serve as a disconnect for this<br />

power supply.<br />

One 120 V, 15Amp, 60 Hz, Single phase connection for control power and power <strong>to</strong> the left side wall<br />

duplex. This circuit should be wired using 14 AWG wire and provided with a 15 Amp circuit breaker that<br />

will serve as a disconnect for this power supply.<br />

One 120 V, 15Amp, 60 Hz, Single phase connection for power <strong>to</strong> the right side wall duplex. This circuit<br />

should be wired using 14 AWG wire and provided with a 15 Amp circuit breaker that will serve as a<br />

disconnect for this power supply.<br />

The circuit breakers for these services are <strong>to</strong> be located in close proximity <strong>to</strong> the cabinet.<br />

Other connections may be required for optional equipment.<br />

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The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> incorporates a solid-state ‘Stedivolt’ controller <strong>to</strong> adjust the blower mo<strong>to</strong>r<br />

speed. This unit compensates for some variation in incoming line voltage. The usable voltage range is<br />

95 <strong>to</strong> 130V AC. You will need <strong>to</strong> consult the most recent certification test report on the unit <strong>to</strong> determine<br />

what the voltage setting should be for your unit.<br />

Environmental Conditions<br />

The <strong>BIOPROtect</strong> <strong>JR</strong> is designed <strong>to</strong> be used under the following conditions:<br />

Indoor use<br />

Altitudes up <strong>to</strong> 2000 meters<br />

Temperature range from 5° C <strong>to</strong> 40° C<br />

Relative humidity up <strong>to</strong> 80%<br />

Main supply voltage 105 <strong>to</strong> 125 VAC<br />

Maximum relative humidity 80 percent for temperatures up <strong>to</strong> 31°C decreasing linearly <strong>to</strong> 50 percent<br />

relative humidity at 40°C.<br />

Transient over voltages according <strong>to</strong> Installation Category(OVERVOLTAGE CATEGORIES) <strong>II</strong> per UL<br />

61010A-1, Annex J.<br />

Pollution Degree 2<br />

Symbols and Terminology<br />

!<br />

Protective Earth: Any terminal which is intended for connection <strong>to</strong> an external protective<br />

conduc<strong>to</strong>r for protection against electric shock in case of a fault.<br />

General Caution: Refer <strong>to</strong> the instruction manual for information regarding personnel and<br />

environment protection.)<br />

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The Uses of the <strong>BIOPROtect</strong> <strong>II</strong> Jr.<br />

<strong>II</strong> - PREPARING THE <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> CABINET FOR USE<br />

The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet has been designed <strong>to</strong> provide a work area that protects the experiment<br />

from the environment (Product protection), and protects the users from the experiment (personnel protection<br />

or ‘containment’). The laminar flow biological safety cabinet is designed for work with Biosafety Levels 1,<br />

2 and 3 (low <strong>to</strong> moderate risk) agents as listed in The Center for Disease Control’s “Biosafety in<br />

Microbiological and Biomedical Labora<strong>to</strong>ries”, U.S. Department of Health and Human Services, Public<br />

Health Service, Centers for Disease Control and National Institutes of Health, U.S. Government Printing<br />

Office, Washing<strong>to</strong>n, D.C. 20402. HHS publication number (CDC) 84-8395.<br />

Extremely high risk agents assigned <strong>to</strong> Biosafety level 4 should never be used in this cabinet except in a<br />

certified BSL4 suit room. Please consult your safety professional for a proper risk assessment.<br />

Cautions<br />

* The use of any hazardous material in the cabinet requires that it be moni<strong>to</strong>red by an industrial<br />

hygienist, safety officer or other qualified individual.<br />

* Explosive or flammable substances should never be used in the cabinet unless a qualified safety<br />

professional has evaluated the risk.<br />

* If hazardous biological work is <strong>to</strong> be performed, apply the appropriate biohazard decal which is<br />

enclosed. This is in accord with OSHA regulations, volume 39, number 125, Part <strong>II</strong>.<br />

* If chemical, radiological or other non-microbiological hazards are present, be sure <strong>to</strong> employ<br />

appropriate protective measures in addition <strong>to</strong> formaldehyde decontamination.Also see section 2<br />

“Decon”. Call upon a suitably trained individual <strong>to</strong> moni<strong>to</strong>r the operation.<br />

Location Within the Labora<strong>to</strong>ry<br />

PROPER PLACEMENT WITHIN THE LABORATORY IS ESSENTIAL.<br />

The ideal location for any laminar flow biological safety cabinet is in a dead-end corner of the labora<strong>to</strong>ry<br />

away from personnel traffic, vents, doors, windows or any other sources of disruptive air currents. Published<br />

research from The <strong>Baker</strong> <strong>Company</strong> (see Rake ASM paper, reference #34 in Appendix) and unpublished tests<br />

performed at the National Cancer Institute show that if a draft or other disruptive air current were <strong>to</strong> exceed<br />

the intake velocity of the cabinet, then contamination can enter the work area or escape from it.<br />

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<strong>II</strong>I - PROPER CABINET USE<br />

The <strong>BIOPROtect</strong> <strong>II</strong> Jr. is designed for continuous operation. The <strong>Baker</strong> <strong>Company</strong> recommends<br />

continuous operation <strong>to</strong> keep the interior work space clean and free of unwanted particles. If the blower is<br />

not running, the workspace of the cabinet will become contaminated with room air. If the cabinet’s use is not<br />

unders<strong>to</strong>od or the cabinet is operated incorrectly, it will not provide an adequate protective barrier.<br />

It would be helpful for opera<strong>to</strong>rs <strong>to</strong> learn about the capabilities and limitations of the cabinet by reading<br />

some of the available literature. The Eagleson Institute offers several videotapes and an interactive CD which<br />

illustrate proper operation techniques for biological safety cabinets.<br />

A biological safety cabinet is a valuable supplement <strong>to</strong> good sterile technique, but is not a replacement for<br />

it. All activities that are <strong>to</strong> be performed in your cabinet should first be approved by a competent<br />

professional, such as an industrial hygienist or safety officer. This will ensure the cabinet is appropriate for<br />

the work it will be required <strong>to</strong> do. A competent professional should moni<strong>to</strong>r the cabinet and its operating<br />

personnel at regular intervals <strong>to</strong> see that it is being used correctly.<br />

Opera<strong>to</strong>r Controls:<br />

The opera<strong>to</strong>r controls and indica<strong>to</strong>rs are arranged on the front panel of the unit.<br />

Blower On / Off Switch –<br />

This push but<strong>to</strong>n is a maintained action switch that controls the power <strong>to</strong> the cabinet blower. Pressing<br />

and releasing the push but<strong>to</strong>n from the UP/OFF position will change the switch <strong>to</strong> the DOWN/ON<br />

position. The cabinet blower will start. The GREEN indica<strong>to</strong>r in the push but<strong>to</strong>n will illuminate. Pressing<br />

and releasing the push but<strong>to</strong>n from the DOWN/ON position will change the switch <strong>to</strong> the UP/OFF<br />

position.<br />

Caution: The cabinet audible alarm is disabled when the Blower switch is in the OFF position<br />

Light On / Off Switch –<br />

This pushbut<strong>to</strong>n is a maintained action switch that controls the power <strong>to</strong> the cabinet fluorescent lights.<br />

Pressing and releasing the pushbut<strong>to</strong>n from the UP/OFF position will change the switch <strong>to</strong> the<br />

DOWN/ON position. The cabinet lights will illuminate. The GREEN indica<strong>to</strong>r in the pushbut<strong>to</strong>n will<br />

illuminate. Pressing and releasing the pushbut<strong>to</strong>n from the DOWN/ON position will change the switch <strong>to</strong><br />

the UP/OFF position.<br />

Mute switch -<br />

This pushbut<strong>to</strong>n is a momentary action switch that temporarily mutes the audible alarm. A timer<br />

module inside the canopy controls the length of time that the alarm is muted. This is set at 5 minutes as a<br />

fac<strong>to</strong>ry default but can be changed <strong>to</strong> 1 second <strong>to</strong> 100 hours. It may be desirable <strong>to</strong> increase this setting<br />

when cabinet decontamination is being performed. The YELLOW indica<strong>to</strong>r in the pushbut<strong>to</strong>n will<br />

illuminate while the alarm mute function is active. Should the alarm condition be remedied before the<br />

mute ‘times-out’ the timer will be reset and the mute indica<strong>to</strong>r will not be illuminated.<br />

Left Side & Right Side Outlet On / Off –<br />

A separate 10 amp breaker switch is supplied for the remote switching of power <strong>to</strong> the right and left<br />

outlet receptacle located inside the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong>. The breaker switches will ‘trip’ if the <strong>to</strong>tal<br />

electrical load on the circuit exceeds 10 amps.<br />

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Ground Fault Circuit Interrupter<br />

Ground fault circuit interrupter (GFCI) outlets are installed on the 115V power circuit on this cabinet.<br />

The GFCI is designed <strong>to</strong> protect the opera<strong>to</strong>r from a possible electrical ground fault hazard. If a<br />

hazardous condition exists the GFCI will cut off electricity <strong>to</strong> the outlet. The but<strong>to</strong>n in the center of the<br />

unit will pop out. If the GFCI “trips” you should first determine and correct the cause of the problem,<br />

then press the but<strong>to</strong>n <strong>to</strong> reset the GFCI.<br />

Air Pressure Alarm<br />

The Air pressure alarm is controlled by a Modus Air Pressure indica<strong>to</strong>r mounted in the control<br />

canopy. Operating & set up instructions for this instrument can be found in the On-Site Checks And<br />

Maintenance section of this manual. When either a High or Low flow condition is present for more than<br />

3 seconds the audible alarm will be activated. Pressing the Mute switch will silence the alarm. The mute<br />

time is determined by the setting of a timer inside the control canopy. Fac<strong>to</strong>ry setting of this timer is 5<br />

minutes. Consult the test report for your cabinet for the fac<strong>to</strong>ry set flow conditions for this alarm. The<br />

<strong>Baker</strong> <strong>Company</strong> recommends continuous operation <strong>to</strong> keep the interior work space clean and free of<br />

unwanted particles.<br />

Caution: The cabinet audible alarm is disabled when the Blower switch is in the OFF position<br />

This feature allows the user <strong>to</strong> turn-off the cabinet blowers when no longer required and the processes<br />

within the cabinet are found <strong>to</strong> be safe <strong>to</strong> personnel and the environment.<br />

Caution: It is recommended that prior <strong>to</strong> turning the Blower OFF you contact your health and safety<br />

officer for decontamination procedures.(also see section 2 “Decon”)<br />

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Door Operations<br />

During cabinet operation, the only access that should be permitted is through the 8” sash opening<br />

provided at the front of the cabinet. For set-up purposes, the viewscreen or the large front door can be opened<br />

<strong>to</strong> provide greater access.<br />

To open the viewscreen:<br />

Caution: Failure <strong>to</strong> latch the viewscreen in the open position may result in injury<br />

Grasp the acrylic panel and pull it away from the door <strong>to</strong> disengage the two grab catches on each side of<br />

the viewscreen.<br />

Swing the panel up <strong>to</strong> engage the support brackets on the cabinet’s <strong>to</strong>p section. You must reach up and<br />

swing the two securing latches down <strong>to</strong> constrain the viewscreen in the open position.<br />

To open the door:<br />

With the viewscreen in the closed position, slide the dead bolt <strong>to</strong> the right <strong>to</strong> disengage the slide from the<br />

door jam. The deadbolt is located just inside the door and mates with a jam located on the left side wall.<br />

Pull the door open. Due <strong>to</strong> the cam rise action of the hinges the door will self close if left open. Swing<br />

the door 100 degrees (slightly more than perpendicular <strong>to</strong> the front of the cabinet) in order <strong>to</strong> override the<br />

self closing feature.<br />

Removing the cart<br />

Caution: It is recommended that prior <strong>to</strong> cart removal you contact your health and safety<br />

officer for decontamination procedures. (also see section 2 “Decon”)<br />

Should it be necessary <strong>to</strong> remove the cart, you must open the front door <strong>to</strong> fully expose the <strong>BIOPROtect</strong> <strong>II</strong><br />

<strong>JR</strong>’s interior work bay. This area is assumed <strong>to</strong> have contamination so use caution in the way you approach<br />

the task.<br />

• Decontaminate and clean the unit as recommended by your health and safety officer.<br />

• Open the door <strong>to</strong> expose the cabinet’s work bay.<br />

• Any equipment on the cart should be disconnected from the side wall service connections and<br />

removed from the cable ports.<br />

• Unlock the front casters by pulling up the foot operated locking devices. When the locking device is<br />

in a horizontal position it is unlocked.<br />

• Use the hand crank <strong>to</strong> lower the height of the work surface if necessary. Check that your equipment<br />

will clear the front header if it is allowed <strong>to</strong> be left on the cart.<br />

• Pull the cart out and away from the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong>.<br />

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Set-up Procedure<br />

This section outlines the tasks required <strong>to</strong> properly install au<strong>to</strong>mated labora<strong>to</strong>ry work stations within<br />

the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong>. This does not replace the recommendations of the equipment manufacturer. It is<br />

assumed that the user is familiar with the set-up and operation of the ancillary equipment.<br />

• Place the equipment on the cart’s table <strong>to</strong>p so that the equipment is within the area of the table <strong>to</strong>p as<br />

defined by the four corners. The front of the equipment must face the front of the cart (crank handle<br />

side).<br />

• Using the crank handle provided on the cart, adjust the cart’s height until the work table of the<br />

equipment is flush or below the intake grill section of the door. S<strong>to</strong>w the crank handle under the work<br />

table.<br />

• Make utility connections <strong>to</strong> the side wall services.<br />

• Close the front door of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> and make certain that you can perform any manual<br />

operations on the equipment through the 8” x 42” sash opening provided.<br />

• Make certain that no part of the equipment blocks or hangs over the front intake grilles.<br />

• Power and run all equipment. Make certain that the equipment and cabinet is operating properly and<br />

that none of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong>’s circuit breakers have ‘tripped’.<br />

• Have a certifier or safety officer witness the air currents (using a smoke tracer) <strong>to</strong> determine that the<br />

equipment does not compromise the cabinet’s ability <strong>to</strong> provide personnel and product protection.<br />

The user may choose <strong>to</strong> use a particle counter <strong>to</strong> evaluate the product protection capacity of the<br />

cabinet. A measurement of the personnel protection capacity of the cabinet (containment), should the<br />

user deem it necessary, will be a more involved test and will require a third party skilled in the<br />

operation of the BIO-analog test or the KI-discus test.<br />

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Start-up procedure<br />

• If the unit has not been left running continuously, press the blower on/off switch. The switch will be<br />

illuminated when switched on. The cabinet’s audible alarm may activate for several seconds until the<br />

blower has come up <strong>to</strong> full speed. Make sure that you have cabinet airflow, either by listening for<br />

blower sound or feeling the airflow across your fingers. Check the reading on the air pressure gauge.<br />

The reading on the gauge should be consistent with the last time the unit was on.<br />

• Turn on the fluorescent light. The switch will become illuminated in the ‘on’ position and the<br />

fluorescent bulbs should activate.<br />

• Wipe down the interior area of the cabinet with a surface disinfectant.<br />

•<br />

• NOTES:<br />

• Some disinfectants may corrode or stain the steel surfaces. If this happens, clean the<br />

• surfaces afterward with a disinfectant-detergent and rinse with sterile tap water <strong>to</strong> prevent corrosion<br />

• Blocking the front and rear perforated grills must be avoided. If wipes or absorbent<br />

• <strong>to</strong>wels are used on the work surface, be sure <strong>to</strong> keep them away from the grills.<br />

• Check that the position of the rear wall dampers (two upper and two lower) are consistent with the<br />

settings from the fac<strong>to</strong>ry test report.<br />

• After your equipment is in place inside the cabinet, close and secure the door. All operations inside<br />

the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> must be carried out through the unit’s 8” sash opening.<br />

•<br />

Caution: The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> will not provide adequate personnel protection unless the front door and<br />

viewscreen are closed.<br />

•<br />

• After a procedure has been completed, all equipment which has been in contact with the research<br />

agent should be enclosed, and the entire surface decontaminated. Trays of discarded items should be<br />

covered. The cabinet should then be allowed <strong>to</strong> run for at least three minutes with no activity so that<br />

the airborne contaminant will be purged from the work area.<br />

• After you have removed all materials, culture apparatus, etc., decontamination of the interior surfaces<br />

should be repeated. Check the work area carefully for spilled or splashed nutrient which might<br />

support bacterial growth. And never use the cabinet <strong>to</strong> s<strong>to</strong>re supplies or labora<strong>to</strong>ry equipment.<br />

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Reacting <strong>to</strong> Spills<br />

Caution: An Emergency spill containment and clean-up procedure should be established<br />

prior <strong>to</strong> an accident<br />

Spilled fluid on the floor of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> is contained within the stainless steel work area of the<br />

cabinet via the threshold placed under the unit’s door. The threshold will contain a volume of approximately<br />

1 gallon (3 liters) of fluid. However, because the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet bears directly on the users floor<br />

there is no drain provided.<br />

• It is recommended that the researchers, in coordination with their consulting safety professional, have<br />

a written plan available in case of an accidental exposure or spill.<br />

• In the case of biological spill, for example, the area containing the spill may be flooded with an<br />

appropriate disinfectant. After the disinfectant has had time for a complete kill, remove the residue. If<br />

you have used a disinfectant which is harmful <strong>to</strong> stainless steel (Hypochlorite bleach solutions, for<br />

example) be sure that none remains <strong>to</strong> corrode cabinet surfaces.<br />

Clean the surfaces with sterile water.<br />

• The Center for Disease Control has published “Biosafety in Microbiological and Biomedical<br />

Labora<strong>to</strong>ries.” If you have a spill involving a hazardous Biosafety Level 2 or 3 agent, then you are<br />

advised <strong>to</strong> leave the cabinet running so as <strong>to</strong> let the aerosols settle before you start cleanup<br />

procedures. With some spills, it may be necessary <strong>to</strong> decontaminate the room with an agent such as<br />

formaldehyde gas. (Biosafety Level 4 agents should never be used in the type of cabinet.)<br />

• If the spill contains volatile liquids which generate vapors creating a danger of fire or explosion, turn<br />

off the unit and other electrical equipment. Evacuate and seal the room and call for immediate help<br />

from a safety professional.<br />

• If the agent is a hazardous chemical, it may be recommended that a Spill Kit be kept readily<br />

available. This kit should be clearly labeled, and might include such items as a respira<strong>to</strong>r, chemical<br />

splash goggles, two pairs of gloves, two sheets of absorbent material, spill control pillows, a solution<br />

<strong>to</strong> clean the contaminant, and waste disposal bags or other containers. Consult your safety<br />

professional for proper procedures and treatment of the specific agents you plan <strong>to</strong> use.<br />

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Decontamination<br />

Whenever maintenance, service or repairs are needed in a contaminated area of your cabinet, the unit<br />

must first be decontaminated by an appropriate agent. The National Institute of Health, National Cancer<br />

Institute and the Center for Disease Control have all recommended the use of formaldehyde gas for most<br />

microbiological agents. Its application requires individuals who are experienced in the decontamination of<br />

cabinets, since the gas itself is <strong>to</strong>xic. Consult the NSF/ ANSI-standard-49-2002 Annex G. “Recommended<br />

Microbiological Decontamination Procedure”, contact NSF International, 789 North Dixboro Road,<br />

P.O. Box 130140, Ann Arbor Michigan 48113-0140.<br />

Whatever gas you choose, have the proper safety equipment (gas masks, protective clothing, etc.) within<br />

easy reach. In addition, you will want <strong>to</strong> be sure that the gas you are using will be effective against all of the<br />

biological agents within the cabinet. When you have decided which gas <strong>to</strong> use, post the antidote <strong>to</strong> it in a<br />

visible and nearby location. The volume of the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet is approximately 75 cubic feet, this<br />

will help you determine the required amount of decontaminating gas.<br />

Carcinogens present a unique chemical deactivation problem and the standard biological decontamination<br />

will not be effective against chemicals or other non-biologic materials. With materials of this kind, consult a<br />

qualified safety professional.<br />

Decontamination procedure<br />

Warning! This procedure should be performed by qualified technicians only.<br />

Surface-disinfect the inside of the window and all other surfaces on the view screen assembly.<br />

Multiply the <strong>to</strong>tal volume of the cabinet (75 ft 3 ) by .3 gram/ft 3 ) <strong>to</strong> determine the amount of<br />

paraformaldehyde required <strong>to</strong> decontaminate the cabinet. If the cabinet is vented <strong>to</strong> the outside you must<br />

consider the volume of the duct work in the paraformaldehyde calculation.<br />

Prior <strong>to</strong> sealing up the cabinet make sure all gas or flammable petcocks are closed and pressure tight.<br />

The inside cabinet work space must be at room temperature with 60% <strong>to</strong> 85% relative humidity. If relative<br />

humidity is low (below 60%) add boiling water in the work space. Moni<strong>to</strong>r the inside of the cabinet with a<br />

humidity meter. Relatively humidity above 85% will require extra clean up which will be covered in step 15.<br />

Note:<br />

Without the proper relative humidity the formaldehyde gas will not be effective. The mode of entry of<br />

formaldehyde in<strong>to</strong> the living organisms is through the cell wall by the absorption of water.<br />

Place a heating mantle with paraformaldehyde in the work space. The heating mantle must be able <strong>to</strong> reach<br />

450 degrees F and must have a grounded plug which should be plugged in <strong>to</strong> an outlet outside the cabinet.<br />

Place a second heating mantle in the cabinet with 60% more by weight of ammonium bicarbonate and 10%<br />

more if using ammonium carbonate, than paraformaldehyde. This will be used later in step 13 <strong>to</strong> neutralize<br />

the formaldehyde gas.<br />

This step is recommended. Place spore strips inside the cabinet <strong>to</strong> confirm the decontamination process has<br />

been successful.<br />

If the cabinet is vented <strong>to</strong> the room, use a sheet of plastic and seal the front access and exhaust port openings.<br />

These openings should be sealed such that the exhaust airflow recirculates back <strong>to</strong> the front access opening.<br />

This will promote the even distribution of formaldehyde gas throughout the cabinet.<br />

If the cabinet is vented <strong>to</strong> the outside, then the exhaust transition should have a means <strong>to</strong> recirculate the<br />

exhaust airflow back <strong>to</strong> the cabinet blower if possible. This will promote the even distribution of<br />

formaldehyde gas through the exhaust filter. Seal the front access opening.<br />

Turn on the heating mantle containing the paraformaldehyde flakes.<br />

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After 25% of the paraformaldehyde has depolymerized, turn on the cabinet blower for 10 <strong>to</strong> 15 seconds.<br />

Repeat after 50%, 75% and 100% of the paraformaldehyde has depolymerized, approx. 20 <strong>to</strong> 30 mins..<br />

Allow the formaldehyde gas a minimum residence time of 6 hours, preferably over night.<br />

Turn on the heating mantle containing ammonium bicarbonate (NH4HCO3). After 25% of the ammonium<br />

bicarbonate has depolymerized turn on the cabinet blower for 10 <strong>to</strong> 15 seconds. Repeat after 50%, 75% and<br />

100% of the chemical has depolymerized <strong>to</strong> neutralize the formaldehyde. Turn on the cabinet blower and<br />

allow the two gases <strong>to</strong> circulate for at least one hour.<br />

Check for formaldehyde by using a gas sampling device (dregger tube) <strong>to</strong> be sure the formaldehyde is at a<br />

safe level inside the lab space.<br />

Cut open the plastic and vent the cabinet <strong>to</strong> the outside.<br />

Aseptically remove spore strip and place in Trypticase-soy broth and incubate for 7 days. No growth will<br />

verify the decontamination process.<br />

When cleaning up, you may find residual paraformaldehyde (white powder) on the metal or glass surfaces.<br />

To remove this, use ammonia in warm water, wear gloves and wipe down the affected surfaces. The<br />

paraformaldehyde will dissolve in water and be neutralized by the ammonia.<br />

•<br />

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About the HEPA Filters<br />

The HEPA filter is one of the essential components of a biological safety cabinet. It is the shield which<br />

stands between the environment and the experimental agent. Some questions that the user should ask<br />

regarding the use of chemical agents in the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> are as follows:<br />

• Are these chemicals, either singly or in combination, able <strong>to</strong> attack filter components?<br />

• Are these chemicals potentially <strong>to</strong>xic <strong>to</strong> the opera<strong>to</strong>r? Is there any combination of two or more which<br />

could be <strong>to</strong>xic? If the cabinet is being correctly used and only the opera<strong>to</strong>r’s hands and arms are<br />

inside the machine, then <strong>to</strong>xicity or irritation could occur through skin penetration. A proper<br />

evaluation of <strong>to</strong>xicity must deal not only with onetime exposure, but also with the effect of many<br />

small exposures over a period of time.<br />

• Are these chemicals explosive or flammable? If so, they should never be used in your cabinet. With<br />

a buildup caused by recirculation of air, an explosion can be the result of a mo<strong>to</strong>r spark or a burner<br />

operation in the work area.<br />

• In cases where chemical carcinogens, mutagens or tera<strong>to</strong>gens are <strong>to</strong> be used, the risks should be<br />

carefully weighed in choosing a cabinet. Where the exhaust effluent contains a contaminant, it may<br />

need treatment.<br />

• The life of a filter is determined by how it is used and how often. Under normal labora<strong>to</strong>ry<br />

conditions, you can expect up <strong>to</strong> five years of use. However, misuse or a heavy dust load within the<br />

cabinet will shorten any filter’s lifetime. Bunsen burners and misuse of chemicals will also shorten<br />

the useful life.<br />

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IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES<br />

We recommend that the filter leak checks be performed before initial use, after relocation and after each<br />

filter change. They should also be carried out at regular intervals, usually six months or one year, as<br />

specified by an industrial hygienist, safety officer or other qualified person.<br />

As reported earlier in this manual, each individual cabinet made by The <strong>Baker</strong> <strong>Company</strong> is carefully<br />

tested before it leaves the fac<strong>to</strong>ry. Your copy of the test report, which you will find at the back of this<br />

manual, gives the fac<strong>to</strong>ry test results for your <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> cabinet.<br />

Use it as your record of the original testing, and as your guide <strong>to</strong> testing in the future. To gain many years<br />

of satisfac<strong>to</strong>ry service; please be sure that your maintenance personnel come as close as possible <strong>to</strong><br />

duplicating these original test figures. Your test procedures should be identical <strong>to</strong> ours so that comparing test<br />

results will have meaning. Please correspond directly with us <strong>to</strong> request detailed procedures for your<br />

particular cabinet model.<br />

Filter Media and Seal Leak Tests<br />

When preparing your cabinet for use after shipment, and then at prescribed intervals throughout its<br />

working life, you will need <strong>to</strong> verify that the filters have maintained their integrity. This is done by scan<br />

testing the filter faces and seals. The Institute of Environmental Sciences recommended practice IES-RP-<br />

CC034.1 would be a good reference for this testing.<br />

The equipment needed will be:<br />

• An aerosol pho<strong>to</strong>meter. The instrument should sample air at a flow rate of 1 CFM.<br />

• A (D.O.P) genera<strong>to</strong>r with Laskin nozzle(s). Liquid dioctylphthalate (DOP), dioctylsebacate or<br />

comparable substance aerosolized by flowing air through the nozzle(s). When generated with<br />

Laskin type nozzle(s), the mean droplet size of the aerosol is 0.1 –3.0 micron with an average of<br />

0.45 micron.<br />

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Procedure for filter leak tests<br />

• Decontaminate the cabinet in accordance with NSF/ANSI standard-49-2002 and your health and<br />

safety officer.<br />

• Remove the front upper panel by first removing the ¼-20 hardware. Remove the damper from the<br />

front of the filter. This will expose the exhaust filter.<br />

• Find the 1/8” NPT hose barb on the <strong>to</strong>p of the cabinet used for measuring the upstream concentration<br />

of challenge.<br />

• Turn on the aerosol pho<strong>to</strong>meter and set-up according <strong>to</strong> the manufacturer's instructions.<br />

• With the cabinet’s blower running, position the D.O.P. genera<strong>to</strong>r so as <strong>to</strong> introduce air generated<br />

smoke in<strong>to</strong> any of the work bay openings (side wall <strong>to</strong> door openings or rear wall openings are all<br />

appropriate for introducing challenge). Measure the upstream concentration of the challenge. You<br />

must have a concentration of at lease 10 micrograms per liter.<br />

• Supply Filter: Remove the diffuser prior <strong>to</strong> scanning the filter. Holding the pho<strong>to</strong>meter probe no more<br />

than one inch from the filter face scan the entire surface area and perimeter (filter gasket frame area)<br />

in slightly overlapping strokes at a traverse rate of not more than ten feet per minute.<br />

• Exhaust filter: You may scan the downstream face of the exhaust filter from outside of the cabinet.<br />

• Eliminate leaks in the gasket frame area by re-tightening the filter gasket.<br />

• An unacceptable leak is defined as a reading greater than 0.01%<br />

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The Airflow Balance<br />

Warning!<br />

This procedure should be performed by qualified technicians only.<br />

The airflow balance which is set at the fac<strong>to</strong>ry provides your unit with the air volume and velocity control<br />

<strong>to</strong> minimize leakage or airborne contamination either in or out of the work area.<br />

In order <strong>to</strong> duplicate as closely as possible the airflow characteristics described in the original fac<strong>to</strong>ry test<br />

report, please follow these steps:<br />

1. Make certain that the front door and viewscreen are closed and secured so as <strong>to</strong> make an 8” x 42”<br />

horizontal access opening.<br />

2. Using a DIM (Direct Intake Measurement) measure the flow rate in<strong>to</strong> the front access opening. Take at<br />

least five (5) consecutive readings. Average the readings and divide by 2.3 square feet (area of the front<br />

access opening). Acceptable range of the average shall be 100-110 FPM.<br />

3. Using a hot wire anemometer mounted on a ring stand within the work area, take readings with the<br />

anemometer probe set 6” below the diffuser. The location of these readings should be the same as shown in<br />

the fac<strong>to</strong>ry test report. The average of these velocity readings must be in the range between 50 and 70 FPM.<br />

4. After you have compared your figures with those originally taken at the fac<strong>to</strong>ry, make whatever<br />

adjustments are necessary. Use the mo<strong>to</strong>r speed control and the exhaust damper <strong>to</strong> bring the intake and<br />

downflow average values within the specifications.<br />

5. As the HEPA filters load up with particulates, airflow will be maintained au<strong>to</strong>matically <strong>to</strong> some extent.<br />

When the air flow is diminished you will have <strong>to</strong> increase the mo<strong>to</strong>r speed <strong>to</strong> maintain the specified intake<br />

and downflow velocity averages. This is done via the speed controller located in the light canopy.<br />

Warning!<br />

When the light canopy is lowered, some electrical parts are exposed. Do not perform this work unless<br />

you are a trained electrician or electronic technician.<br />

!!!!Note that terminals M1 & M2 as well as L1 & L2 are high Voltage.!!!!<br />

On the speed controller, turn the potentiometer clockwise until you have the desired airflow. Use a<br />

voltmeter <strong>to</strong> check the voltage across terminals M1 & M2. This is the voltage out <strong>to</strong> the mo<strong>to</strong>r. If the airflow<br />

cannot be maintained, it will necessary <strong>to</strong> replace the HEPA filters.<br />

(See “Procedure for HEPA Filter Replacement” later in this section.).<br />

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Airflow Smoke Pattern Test<br />

Airflow smoke patterns have been shown <strong>to</strong> vary depending on the equipment that is used inside the<br />

cabinet. The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> has been tested with the cart only. A description of this testing is<br />

summarized in the test report. However, when the <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> is set-up properly (i.e. your set-up<br />

should not block or restrict the grilles and diffusers provided) the appearance of the airflow directly at the 8”<br />

access openings must be consistent with that described in the test report.<br />

To check for the direction of air movement, use a smoke genera<strong>to</strong>r and trace along the front access<br />

opening on the inside of the cabinet. Observe that no smoke is escaping from the work area.<br />

In order <strong>to</strong> be sure that room air is not entering the work area, trace along the outside of the front access<br />

opening. Observe that no smoke penetrates <strong>to</strong> the work surface.<br />

Grounding Continuity Test<br />

Using an Ohmmeter, set it <strong>to</strong> read the low resistance scale. Touch the two leads <strong>to</strong>gether and see that the<br />

display reads 0.1Ω <strong>to</strong> 0.0Ω. Touch one lead <strong>to</strong> the ground lug on the cabinet power cord while <strong>to</strong>uching the<br />

other lead <strong>to</strong> bare metal on the unit where the user would be likely <strong>to</strong> <strong>to</strong>uch the cabinet. According <strong>to</strong> NSF/<br />

ANCI-standard 49-2202 the maximum allowable shall be 0.15Ω (Max allowable for UL is 0.50Ω ).<br />

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Calibration procedure for Modus Airflow alarm(Digital Differential Pressure Moni<strong>to</strong>r).<br />

Note: This procedure MUST be followed every time the damper width has been changed.<br />

Initial Set Point Settings<br />

Balance the cabinet air flows <strong>to</strong> nominal operating set point per manufacturer instruction.( See<br />

instruction label on front of cabinet).<br />

Record voltage <strong>to</strong> mo<strong>to</strong>r/blower (M1, M2 on speed control) and measure and record damper/ slot<br />

width<br />

Make certain that the Modus displays 0.000 when zero pressure is applied(This can be easily<br />

accomplished by removing the Tygon tubing from the hose barb fitting). Use the “zero” function if<br />

necessary.<br />

Re-connect the tygon tubing and make sure that the value displayed is negative(-) when the cabinet is<br />

running.<br />

Low Flow Alarm Setting<br />

Place a DIM airflow hood on the front of the cabinet or use the 3 inch method for measuring inflow.<br />

Adjust cabinet <strong>to</strong> a 20% reduction in airflow by decreasing the voltage <strong>to</strong> mo<strong>to</strong>r. DO NOT adjust the<br />

damper<br />

Record the exhaust/ damper pressure on Modus (indication of exhaust plenum pressure at<br />

20% reduction) and voltage <strong>to</strong> mo<strong>to</strong>r/ blower (across M1, M2 on speed control)<br />

Set the Modus High Alarm set point <strong>to</strong> the 20% reduction in pressure recorded in step 14 (per Modus<br />

programming instructions below)<br />

Set the Modus dead band <strong>to</strong> .002 (per Modus programming instructions below).<br />

Return cabinet <strong>to</strong> Nominal Set Point voltage, recorded in step 2<br />

Check the Calibration<br />

Set Point Check<br />

While measuring the intake airflow with a DIM, reduce the airflow by decreasing the voltage <strong>to</strong><br />

mo<strong>to</strong>r/ blower until a 20% reduction in flow is measured. Check that the low set point alarms at this<br />

value. If not, reset Modus low alarm set point <strong>to</strong> alarm as desired, per instructions. Return cabinet<br />

<strong>to</strong> Set Point Voltage.<br />

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Modus Programming Instructions<br />

1.) Programming the Alarm Set points<br />

After the first press of MODE, the HI LED flashes, indicating the HI set point is being entered. Press<br />

the ARROW key repeatedly <strong>to</strong> display the desired digit. Next, press the MODE <strong>to</strong> move <strong>to</strong>wards the<br />

most significant digit, and repeatedly press the ARROW key <strong>to</strong> advance the digit’s value. Continue<br />

for the remaining digits. The sign of the set point (+ or -) is selected last.<br />

After the HI set point has been programmed, press the MODE <strong>to</strong> enter the LO set point. The LO<br />

alarm LED flashes, and you can repeat the above process <strong>to</strong> program the LO set point.<br />

2.) Programming the Relay Dead band<br />

The dead band selection is provided <strong>to</strong> keep the relays from chattering when the pressure varies near<br />

the set point. To display the dead band, press MODE once after setting the LO set point polarity in<br />

the previous step. Both alarm LEDs flash. The three digits displayed are the dead band width, in the<br />

same units used for data display. Press the ARROW and MODE keys as described earlier <strong>to</strong> step the<br />

dead band <strong>to</strong> a value of .002.<br />

3.) Returning <strong>to</strong> Normal Operation<br />

Press MODE once after the setting the dead band parameters. The digit “1” on the left indicates the<br />

unit is in analog setting mode. Since there are no analog outputs, the mode key can be pressed a final<br />

time <strong>to</strong> end programming and return <strong>to</strong> the normal pressure display.<br />

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Maintenance Notes<br />

Replacing the HEPA Filter<br />

WARNING!<br />

Before any panels are removed, the cabinet must be decontaminated. The filters are sure <strong>to</strong> have<br />

collected microorganisms and other potentially harmful particles generated from the work area during<br />

their lifespan. Maintenance personnel should not allow themselves <strong>to</strong> be exposed.<br />

It should also be remembered that a specific gaseous decontaminant may work against<br />

microorganisms, but not against chemical agents. Where chemical agents are present, consult an<br />

industrial hygienist or other qualified person(s).<br />

If the differential pressure moni<strong>to</strong>r presents an alarm, or if your periodic checks of <strong>to</strong>tal airflow show a<br />

drop of more than 5% of the nominal set point, the filters may be loading with particulates. As explained<br />

earlier in this section, the blower speed can be manually increased <strong>to</strong> compensate for filter loading.<br />

However, when the airflow can no longer be maintained or when the filters are damaged, they need <strong>to</strong> be<br />

replaced.<br />

A contaminated filter must be handled with caution. Personnel should be protected by clothing or<br />

breathing apparatus as necessary <strong>to</strong> the nature of the hazard. It is advisable <strong>to</strong> seal the contaminated side of<br />

the filter by taping a plastic sheet or cardboard over the face before removal. This should minimize the<br />

number of particles shaken loose from the filter. Once removed, the filter should immediately be sealed in a<br />

biological hazard bag and then disposed of safely in accordance with environmental regulations.<br />

After filter replacement has been completed, the cabinet and the room should be cleaned and<br />

decontaminated in a manner consistent with the nature of the hazardous material. The cleaning materials,<br />

along with the protective gear and clothing, should be properly disposed of.<br />

HEPA filters are very easily damaged, and you will want <strong>to</strong> use great care during handling so as <strong>to</strong> avoid<br />

injury <strong>to</strong> the filter media and gasket surface. When installing the new filters, it is a good idea <strong>to</strong> tape a piece<br />

of cardboard over the filter media <strong>to</strong> give protection against dropped wrenches or misdirected fingers. Of<br />

course, you will need <strong>to</strong> make sure that the cardboard is removed before the access panels are re-installed.<br />

Inspect the filters carefully before and after installation. A broken or damaged filter is worthless.<br />

Changing the Exhaust Filter<br />

The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> requires one exhaust filter measuring 19” x 38” x 12” deep (consult test report<br />

for part numbers and specifications). This filter is arranged <strong>to</strong> face the front of the unit so as <strong>to</strong> eliminate<br />

the nuisance of ceiling restrictions on the exhaust air.<br />

To service the exhaust filter remove the panel from the front of the unit using a 3/8” nut driver or<br />

socket <strong>to</strong> remove the ¼-20 hardware. Using the same 3/8” driver, remove the hardware from the filter<br />

securing frame. Pull the frame and filter away from the unit.<br />

Prepare the new filter by putting a light coat of silicone grease on the face of the gasket. Clean the<br />

sealing flange thoroughly. Then carefully slide the new filter in<strong>to</strong> place and make sure that it is properly<br />

seated on the flange. Replace the filter clamp assembly and perform the leak test described earlier in this<br />

chapter.<br />

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Changing the Supply Filter<br />

The <strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong> requires one supply filter measuring 32” x 45” x 3” deep (consult test report<br />

for part numbers and specifications). This filter is arranged so as <strong>to</strong> be serviced from inside the<br />

<strong>BIOPROtect</strong> <strong>II</strong> <strong>JR</strong>’s work area. Consequently you will have <strong>to</strong> remove the cart and any equipment from<br />

inside the cabinet prior <strong>to</strong> changing the filters.<br />

To service the supply filter first swing the diffuser down allowing it <strong>to</strong> rest on the rear wall by<br />

removing the ¼-20 screws at the front edge of the diffuser panel. Next using a 3/8” driver remove the ¼-<br />

20 hardware that secures the frame <strong>to</strong> the filter. This frame supports the filter.<br />

Prepare the new filter by putting a light coat of silicone grease on the face of the gasket. Clean the<br />

sealing flange thoroughly. Place the new filter in the center of the frame and install the frame in<strong>to</strong> the<br />

cabinet using the ¼-20 hardware. Conduct a filter leak check as described in the earlier chapter.<br />

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V. - TROUBLE SHOOTING<br />

Here are some suggestions based on our experience with the use and misuse of biological safety<br />

cabinets.<br />

CAUTION:<br />

Whenever the potentially contaminated areas of your cabinet must be entered, make sure that the unit is<br />

first decontaminated by use of an appropriate method by qualified personnel.<br />

When a smoke test indicates that there is air flowing from the interior of your cabinet in<strong>to</strong> the<br />

surrounding room:<br />

• Make certain that the front door is closed and the front intake grilles are not blocked. This will create<br />

an 8” x 42” horizontal opening in the front of the cabinet.<br />

• If your cabinet is connected <strong>to</strong> an in-house exhaust, make sure that there is adequate exhaust suction<br />

and no back pressure. Also, be sure the dampers are open. Re-balance the exhaust system <strong>to</strong> handle<br />

an adequate quantity of air and static pressure (suction). Consult with building maintenance people.<br />

• The exhaust filter may be loaded with dirt if the unit has been in service for some time.<br />

Decontaminate, and replace the HEPA filter.<br />

• There may be high cross-drafts in the room which are causing the outflow of smoke. Check the<br />

airflow balance, following the procedure recommended in an earlier section of this chapter. Eliminate<br />

the source of the cross-draft.<br />

If there is no airflow within the work area:<br />

• Make sure that the blower switch is turned on. The indica<strong>to</strong>r on the pushbut<strong>to</strong>n should be<br />

illuminated.<br />

• If the lights are working, then turn the blower switch off and let the cabinet rest for ten minutes.<br />

When the time has passed, turn the blower switch on again. If the blower starts, you know there has<br />

been overheating of the blower mo<strong>to</strong>r. Also check the wiring connections inside of the light canopy<br />

which connects the canopy <strong>to</strong> the cabinet. Be sure the connections are pushed <strong>to</strong>gether.<br />

• If these solutions do not correct the problem, or if the blower failed <strong>to</strong> start after the rest period, then<br />

either the speed control, blower mo<strong>to</strong>r or capaci<strong>to</strong>r is defective. A qualified electrician can find out if<br />

the speed control is defective by bypassing it, using the wiring diagram in the Appendix of this<br />

manual as a guide. If there is a noise problem, it may be caused by mo<strong>to</strong>r bearings.<br />

When there is uneven fan operation, or noise from the mo<strong>to</strong>r/blower assembly:<br />

• It may be necessary <strong>to</strong> decontaminate the cabinet.<br />

• Look for loose objects in the fan cage.<br />

• Check <strong>to</strong> see if the fan wheel is contacting the blower housing.<br />

• Open the light canopy and check the multi-pin connec<strong>to</strong>rs at each end <strong>to</strong> be sure they are securely<br />

engaged.<br />

• Another possible source of the problem is the speed control.<br />

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If the fluorescent light does not work -<br />

• Make sure that the light switch is turned on. The indica<strong>to</strong>r on the pushbut<strong>to</strong>n should be illuminated.<br />

• Make certain that the bulbs are installed properly in<strong>to</strong> the clips.<br />

• Open the light canopy and check the multi-pin connec<strong>to</strong>rs at each end <strong>to</strong> be sure they are securely<br />

engaged. If the lamp flickers, there is probably an improper ground. The wiring can be traced <strong>to</strong> the<br />

source of a break.<br />

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REPLACEMENT PARTS LIST<br />

PART NAME QTY<br />

BAKER COMPANY<br />

PART NUMBER<br />

Blower 1 11432<br />

Capaci<strong>to</strong>r 1 11558<br />

Mo<strong>to</strong>r (120 V, ½ HP, 1620 RPM) 1 31679<br />

Filter, exhaust, 19” x 38” x 12” 1 41175<br />

Filter, supply, 32” x 45” x 3” 1 38913<br />

Power Supply, 24V DC, 1A 1 39324<br />

Mo<strong>to</strong>r speed control 1 39658<br />

Fluorescent lamp 2 17927<br />

Ballast, fluorescent 1 34160<br />

Pressure Moni<strong>to</strong>r/Alarm 1 38996<br />

Hydraulic pump kit (cart) 1 40555<br />

NOTE:<br />

When ordering replacement parts, please furnish serial number and model number of unit<br />

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VI. APPENDIX<br />

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Modus Installation and Operation Handbook<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 1<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 2<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> <strong>Vent</strong> <strong>to</strong> Room Page 3<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 1<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 2<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Canopy Exhaust Connection(CEC) Page 3<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 1<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 2<br />

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BGA-BIOPROTECT <strong>II</strong>-<strong>JR</strong> Hard Exhaust Connection(HEC) Page 3<br />

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BIOPROTECT <strong>II</strong>-<strong>JR</strong> OPTIONAL CART<br />

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Wiring Diagram-Standard <strong>BIOPROtect</strong> <strong>JR</strong><br />

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Wiring Diagram – Master <strong>BIOPROtect</strong> <strong>JR</strong><br />

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WARRANTY<br />

The <strong>Baker</strong> <strong>Company</strong>, Inc. expressly represents and warrants all goods (a) <strong>to</strong> be as specified in the <strong>Baker</strong><br />

<strong>Company</strong> catalogs and literature, and (b) <strong>to</strong> be free under normal use, service and testing (all as described in<br />

the <strong>Baker</strong> <strong>Company</strong> catalogs and literature) from defects in material and workmanship for a period of twelve<br />

months from the invoice date.<br />

The exclusive remedy for any breach or violation of this warranty is as follows: The <strong>Baker</strong> <strong>Company</strong>,<br />

Inc. will, F.O.B. Sanford, Maine furnish without charge repairs <strong>to</strong> or replacement of the parts or equipment<br />

which proved defective in material or workmanship. No claim may be made for any incidental or<br />

consequential damages.<br />

This warranty is expressly in lieu of all other warranties, express or implied, including any implied<br />

warranty of merchantability or fitness for a particular purpose, unless otherwise agreed in writing<br />

signed by the <strong>Baker</strong> <strong>Company</strong>. (The <strong>Baker</strong> <strong>Company</strong> shall not be responsible for any improper use,<br />

installation, service, or testing of the goods).<br />

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