OPERATOR'S MANUAL BIOPROtect II JR Vent to ... - Baker Company

I - FUNCTION AND DESCRIPTION OF THE BIOPROtect II JR CABINET
The BIOPROtect II JR is a Class II Bio-Containment Enclosure designed to house laboratory robotics or
large apparatus that would not ordinarily fit in a standard Class II Biological Safety Cabinet (BSC). Some
important features of the BIOPROtect II JR include, vertical laminar airflow, a front access opening, as well
as supply & exhaust HEPA filters. The unit is designed to protect not only the environment and the people
using the cabinet, but also the product within from airborne contaminants. The BIOPROtect II JR can be
configured to vent three different ways. Vent to the room, Vent to a Canopy Exhaust Connection (C.E.C.)
via a house exhaust system or, Vent to a Hard Exhaust Connection (H.E.C.) via a house exhaust system.
Although your unit has passed a microbiological test, a modification of NSF/ANSI standard-49-2002e, the
BIOPROtect II JR is not currently listed with the NSF.
Airflow Inside the BIOPROtect II JR Cabinet
Figure 1 shows the name and location of component parts and the typical airflow pattern of the
BIOPROtect II JR
The stainless steel metal diffuser (1) just below the supply HEPA (High Efficiency Particulate Air) filter
assures that the work area is bathed in HEPA filtered air. The supply air stream splits so that some is
directed into the front grille (2) and the balance is pulled into the perforated grilles on the rear wall (3). On a
flat table top, in the absence of any obstacles, the air should split roughly in the center of the table.
Because the cabinet must take in air to replace the air which is expelled through the exhaust filter, the
same quantity of room air enters the cabinet through the eight inch front access opening. The flow of the
intake air provides the personnel protection (containment) aspect of the cabinet.
The front grille of the cabinet is integral to the large hinged door. This door, as well as the side and rear
walls, are maintained under a negative pressure. Air is drawn from the front intake grille, into the hollow
door, and into the right side wall. In this manner the outside air that is drawn into the cabinet as intake is not
allowed to penetrate the table top area of the cabinet.
Any and all contaminated air that is captured in the cabinet’s negative pressure plenums (front door, side
walls, rear walls or upper plenum) is pulled into the cabinet’s blower (4). The blower discharges into a steel
box that is a common plenum for the supply and exhaust HEPA filters (5). In this manner, all the air that is
handled by the cabinet is HEPA filtered. The BIOPROtect II JR will exhaust a quantity of air (6) equal to
that received at the front intake grille. If a leak should occur in a contaminated pressure plenum the negative
pressure will create suction and pull air in, not allowing it to escape into the laboratory. If there is a leak
from the positive pressure plenum, the surrounding negative pressure area will recapture the contaminated air
and recirculate it through a HEPA filter.
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