Cambridge Pre-U Syllabus - Cambridge International Examinations

More documents

Recommendations

Info

22 Cambridge Pre-U Draft Practical learning outcomes Candidates should be able to: i) investigate genetics using locally available materials (e.g. locally available plants), germinating seedlings (e.g. rapid-cycling Brassica), Drosophila, fungi (such as Sordaria fimicola), genetic tomatoes, prepared materials such as ‘genetic corn-cobs’ and any other materials that yield suitable numerical information ii) investigate continuous and discontinuous variation with any available materials (e.g. people, plants with suitable single-gene and polygenic characteristics, polymorphic snails, etc.) 1.7 Applications of cell biology Content Principles of genetic engineering Isolating genes Cloning DNA Vectors and insertion into host cells Identifying and cloning transformed cells Gene therapy and genetic profiling (DNA fingerprinting) Gene sequencing – methods and applications Stem cells – isolation and uses Ethical issues surrounding genetic engineering and the use of stem cells Learning outcomes Candidates should be able to: a) discuss the potential and actual advantages and disadvantages of transferring genetic material by genetic engineering compared to selective breeding b) explain why promoters and other control sequences may have to be transferred as well as the desired gene c) explain strategies that are available to isolate the desired gene from the genome of the gene-donor including: • use of mRNA and reverse transcriptase • use of restriction endonucleases to fragment the genome, and use of electrophoresis and complementary gene probes to identify relevant fragments from the gene d) outline the principles of PCR as used to clone and amplify DNA and discuss the source and importance of Taq polymerase e) explain strategies that are available to insert DNA into host cells including: • inserting the DNA into a plasmid vector using restriction enzymes and DNA ligase and then inserting the plasmid vector into a host cell • use of Agrobacterium tumefaciens in inserting DNA into dicotyledonous plant cells • use of microprojectiles in inserting DNA into monocotyledonous plant cells (e.g. in creating Golden Rice TM and Golden Rice 2) f) discuss the advantages and disadvantages of ways that have been used to identify transformed cells including antibiotic resistance genes and green fluorescent protein (GFP) genes g) outline how genes are inserted into target cells in gene therapy (limited to liposomes and viral vectors) h) explain the limitations, both potential and actual, of gene therapy as a treatment for genetic conditions (including cystic fibrosis and severe combined immunodeficiency [SCID])
Cambridge Pre-U Syllabus i) outline the processes used in genetic profiling (DNA fingerprinting) including the use of restriction endonucleases, amplification, electrophoresis visualisation (e.g. by fluorescently tagged primers), and match tables j) describe methods of DNA sequencing (limited to the chain termination and the dye-terminator methods) and describe uses of this technology (to include the Human Genome Project and uses in taxonomy (molecular phylogenetics)and clinical diagnosis) k) describe how stem cells (zygotic, embryonic and adult) are obtained for research l) discuss the current and potential uses of stem cells (e.g. replace damaged tissues, study aspects of development and cell chemistry, test new drugs, screen potentially toxic chemicals, facilitate gene therapy) m) discuss the ethical implications of the applications of genetic engineering and stem cells, including agricultural, industrial, research and medical applications. Practical learning outcomes Candidates should be able to: i) investigate aspects of genetic profiling including practical investigation of electrophoresis using dyes and DNA fragments ii) investigate transformation of bacteria, e.g. using the pGLO plasmid iii) investigate the lac operon using ONPG solution 23
Page 1: Cambridge Pre-U Syllabus - DRAFT Su
Page 4 and 5: 2 © University of Cambridge Intern
Page 6 and 7: 4 Introduction Cambridge Pre-U Draf
Page 8 and 9: 6 Aims The course aims to: Cambridg
Page 10 and 11: 8 Assessment objectives Cambridge P
Page 12 and 13: 10 Description of Papers Cambridge
Page 14 and 15: 12 Curriculum Content The syllabus
Page 16 and 17: 14 Cambridge Pre-U Draft In the con
Page 18 and 19: 16 1.1 Eukaryotic cell structure Co
Page 20 and 21: 18 1.3 Cell replication Content DNA
Page 22 and 23: 20 1.5 Respiration Content ATP Chem
Page 26 and 27: 24 2. THE ORIGIN AND EVOLUTION OF L
Page 28 and 29: 26 2.3 The evolution of life Conten
Page 30 and 31: 28 3. ANIMAL PHYSIOLOGY Section 3 (
Page 32 and 33: 30 3.3 Nerves, muscles and behaviou
Page 34 and 35: 32 3.5 The immune system Content St
Page 36 and 37: 34 4. THE LIFE OF PLANTS Cambridge
Page 38 and 39: 36 4.3 Reproduction Content Pollina
Page 40 and 41: 38 5. ENVIRONMENTAL STUDIES Cambrid
Page 42 and 43: 40 5.2 Measuring and conserving bio
Page 44 and 45: 42 Cambridge Pre-U Draft Section A
Page 46 and 47: 44 Assessment of skills in Componen
Page 48 and 49: 46 Cambridge Pre-U Draft Candidates
Page 50 and 51: 48 Cambridge Pre-U Draft Sub-skill
Page 56 and 57: 54 Cambridge Pre-U Draft Skill Area
Page 58 and 59: 56 Cambridge Pre-U Draft Laboratory
Page 60 and 61: 58 Cambridge Pre-U Draft Reagents,
Page 62 and 63: 60 Appendix 2: Textbooks and IT res
Page 64 and 65: 62 Cambridge Pre-U Draft Biology Pr
Page 66 and 67: 64 Notes on the use of statistics i
Page 68 and 69: 66 Cambridge Pre-U Draft Glossary o
Page 70 and 71: 68 Appendix 4: Performance Descript
Page 72 and 73: 70 Appendix 5: Additional informati
Page 74 and 75:
72 Cambridge Pre-U Draft
show all

Cambridge Pre-U Syllabus - Cambridge International Examinations

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?