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Cambridge Pre-U Syllabus - Cambridge International Examinations

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<strong>Cambridge</strong> <strong>Pre</strong>-U <strong>Syllabus</strong><br />

Bench lamp with flexible arm<br />

Balance (to 0.01 g)<br />

Water-baths or equivalent<br />

Cork borers<br />

Stopclock/timer showing seconds<br />

Simple respirometer – can be ‘homemade’<br />

Pipe cleaners/other suitable aid to demonstrate mitosis and meiosis<br />

Apparatus to measure rate and depth of breathing<br />

Petri dishes, culture bottles, autoclave<br />

Inoculating wires/bioloops<br />

Haemocytometers<br />

Tape for sealing dishes<br />

Stocks of the following chemicals and reagents:<br />

Iodine in potassium iodide solution<br />

Benedict’s solution<br />

[C] – biuret reagent/potassium hydroxide and copper sulfate solution<br />

[F] – ethanol (for fats test)<br />

[F] – methylated spirit (extraction of chlorophyll)<br />

Sucrose (use AR for non-reducing sugar test)<br />

Glucose<br />

Starch<br />

[C] – Potassium hydroxide<br />

Sodium chloride<br />

Dilute hydrochloric acid<br />

Hydrogencarbonate indicator<br />

Sodium bicarbonate/sodium hydrogencarbonate<br />

Limewater<br />

Distilled/deionised water<br />

Universal Indicator paper and chart<br />

Litmus paper<br />

Neutral red solution or powder<br />

[H] – Eosin/red ink<br />

[H] – Methylene blue<br />

Vaseline/petroleum jelly (or similar)<br />

DCPIP (dichlorophenol-indophenol)<br />

Ascorbic acid (vitamin C)<br />

[H] – Enzymes: amylase, trypsin (or bacterial protease), pepsin, pectinase<br />

Materials for preparing immobilised enzymes: calcium chloride, sodium alginate<br />

Potatoes (store in fridge) or mung beans (to germinate for use) as a source of catalase<br />

Non-competitive enzyme inhibitor (e.g. copper sulfate)<br />

Stains for preparing slides to show mitosis – e.g. carmine acetic, toluidine blue, aceto-orcein<br />

[H] – Feulgen stain (Schiff’s reagent)<br />

[H] – Reagents for Gram staining – solutions of crystal violet, Gram’s iodine and safranin<br />

Reagents for paper or thin layer chromatography<br />

Nutrient broth, nutrient agar<br />

Reagents and enzymes for investigation of the lac operon – see, for example:<br />

www-saps.plantsci.cam.ac.uk/worksheets/scotland/lac.htm<br />

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