XXII Annual Scientific Meeting, Tucuman Biology Society ...

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172 ABSTRACTS 25. BIOCHEMICAL CHARATERIZATION OF A RECOMBI- NANT α-GALACTOSIDASE FROM Lactobacillus fermentum CRL 722 LeBlanc JG 1 , Carrera Silva EA 1 , Sesma F 1 , Savoy de Giori G 1,2 . 1 CERELA-CONICET. Chacabuco 145. Tucumán, Argentina. (T4000ILC). E-mail: leblanc@cerela.org.ar. 2 UNT. Cat. Microbiol. Superior. Tucumán, Argentina. The use of microbial α-galactosidase (α-Gal) which hydrolyzes α- 1,6 galactoside links is a promising alternative to eliminate αgalactooligosaccharides (α-GOS) found in soy-derived products. These sugars often pass through the small intestine and reach the large intestine where they are fermented by the intestinal microbiota, producing gases that can cause physiological problems in sensitive individuals. The goal of this study was to characterize the α-Gal of Lb. fermentum CRL722 produced by E.coli. The α-Gal gene was amplified using degenerate primers, sequenced and cloned into E. coli. The recombinant enzyme was purified and compared to the α-Gal from the original strain (CRL722), both being active in a wide range of pH and temperatures and in the presence of various ions. The complete gene will now be amplified and inserted in probiotic microorganisms which would then be able to degrade α-GOS in situ in the gastrointestinal tract or use to produce novel fermented foods. 26. INFLUENCE OF YOGURT CONSUMPTION ON IMMUNE RESPONSE TO Streptococcus pneumoniae INFECTION Racedo S 1 , Villena J 2 , Agüero G 2 , Alvarez S 1,2 *. 1 CERELA-CONICET. Chacabuco 145. 2 Instituto de Bioquimica Aplicada.UNT. S. M. de Tucumán. (4000) Tucumán. Argentina. *Email: salvarez@cerela.org.ar Streptococcus pneumoniae (Sp) may result in asympto- matic carriage, mucosal or invasive disease. Recent studies have given support that yogurt consumption may indeed be beneficial to health and it has been suggested that it may increase pathogen cleareance and reduce lung injury. The aim of this work was to examine the influence of yogurt consumption on local immune response of mice infected with Sp. Conventional balance diet of swiss-albine adult mice was supplemented for 5 consecutive days with yogurt (5dy). The local immune response was evaluated before and after challenged with Sp (10 6 CFU ml -1 mouse -1 ) during 15 days postinfection (dpi): Total and differential blood (B) and broncho alveolar (BAL) leukocyte counts; Mieloperoxidase (MPO) activity in B and lung homogenate (LH); % of Nitro blue tetrazolium (NBT)+ phagocityc cells from BAL and IgA+ producing cells in BALT (bronchus associate lymphoid tissue) by IF. The 5dy group showed increase of the number of BAL leucocytes at 1dpi. (p
BIOCELL 30(1), 2006 ABSTRACTS 173 29. PREVENTIVE ADMINISTRATION OF LACTIC ACID BACTERIA IN Streptococcus pneumoniae INFECTION MODEL. POTENTIAL USE AS VACCINE VECTORS Villena J 1 , Medina M 1,2 , Alvarez S 1,2 *. 1 Inst. Bioq. Aplicada. UNT. Balcarce 747. 2 CERELA. Chacabuco 145. Tucumán (4000).*E-mail: salvarez@cerela.org.ar We studied the adjuvant effect of Lactococcus lactis NZ9000 (Ll) and Lactobacillus plantarum 37A (Lp), orally administered, in a Streptococcus pneumoniae (Sp) respiratory infection. Swiss-albine adult mice were treated with Ll or Lp (10 7 , 10 8 or 10 9 UFC/day) for 2, 5 and 7 days (d). At the end of each treatment mice were infected intranasally with Sp (10 6 UFC per mouse) and during 15 d postinfection we performed: A)Bacterial cells counts in lung and blood samples, B)Hematocrit and haemoglobin, C)Total and differential leucocytes counts in blood and bronco-alveolar lavages (BAL). Results: Only Ll administered for 5d in a dose of 10 8 (Ll5d8) was able to reduce significantly the pathogen counts in lung and blood samples. The number of leucocytes and neutrophils in blood and BAL increased significantly in all groups after the challenge, but mice treated with Ll5d8 showed higher levels than the controls. Lp failed to improve resistance against Sp. Conclusion: The oral administration of Ll in the appropriate dose, improves the resistance against Sp, by inducing a stronger recruitment of inflammatory cells. This immunoadjuvant effect and their ability to be easily transformed made Ll a good candidate as delivery system for mucosal vaccination. 30. EFFECT OF LACTOBACILLUS CASEI ADMINISTRATION ON D-GALACTOSAMINE HEPATOTOXICITY: ROLE OF KÜPFFER CELLS Lazarte S, Vintiñi E, Alvarez S, Agüero G. FBQF, FAZ-UNT. CERELA-CONICET. Chacabuco145. S. M. de Tucumán. E-mail: gaguero@fbqf.unt.edu.ar We have demonstrated that Lactobacillus casei (Lc) administration prevents the bacterial translocation (BT) from the gut in acute liver injury induced by D-Galactosamine (D-Gal) in BALB-c mice. The Küpffer cells (KC) are hepatic macrophages and their increase would be an important event in the D-Gal hepatotoxicity. Because the bacterial endotoxin activates KC, we want to study its participation of in D-Gal hepatotoxicity and to determine the effect of Lc administration on KC. Adults BALB-c mice were injected with D- Gal (DG) another group were orally fed with Lc during 2 days previous to D-Gal injection (Lc+DG). Control mice with and without Lc, (Lc) and (NC) respectively, were inoculated with saline solution. Every group was injected with china ink and afterward liver was taken for biopsy to count the KC. Liver injury was tested by serum GPT and tissue damage. The hepatic architecture was normal in every group. However, the GPT and CK were increased in DG and Lc+DG groups respect to the NC and Lc groups (p
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