XXII Annual Scientific Meeting, Tucuman Biology Society ...

174 ABSTRACTS
33.
CLINICAL ALTERNATIVE TO REESTABLISH BIOLOGI-
CAL SPACE. PRELIMINARY CLINICAL STUDY
Valdivia G, Montero CA, Ballesteros G, Aragón HN, Gordillo ME,
Loi JA.
Periodontics Chair, University of Odontology, U.N.T. (National
University of Tucumán). E-mail: joseloi@odontologia.unt.edu.ar
Biological conditions represent the key factor for the clinical treatments
of periodontitis. Several authors reported non -surgical and
surgical treatments. Objectives: preliminary information about a
non-invasive procedure, preserving a normal area which allows the
reinsertion of collagen and the adhesion of the epithelium union,
thus re-establishing the biological space in a new position. Materials
and Methods: seven single permanent roots were selected, in
which forceps were used for their luxation and supragingival removal;
then they were splinted in a semi -rigid way. Clinical records
were taken in relation to the gingival margins of neighbour teeth.
The radiographic measures registered the distance between alveolar
bone crest and the enamel -cement limit in mesial and distal
relation to the neighbour teeth. Results: with Friedman test (p >
0.05) no significant changes were seen. Radiographic analysis (twoway
ANOVA), didn’t show changes between groups neither (p >
0.05). The mobility variable doesn’t show any change after 60 days.
Conclusions: this technique preserves aesthetics, biology, and can
be performed by the general dentist.
Subsidized by C.I.U.N.T. (Investigation Center of the National University
of Tucumán).
34.
REPRODUCTION IN MARSUPIAL FROG Gastrotheca
christiani (Amphibia, Anura)
Pucci Alcaide A, Alcaide M.
Fundación Miguel Lillo, Tucumán. 4000.
E-mail: felisaalcaide@hotmail.com
Gastrothece Christiani has a particular reproductive mode, his eggs
are carried in a female dorsal incubatory pouch, form by a skin
fold. This paper is the histomorphological and histochemical study
of the ovary, oviducts, and the embryo development in the pouch;
in order compared with other species of the Gastrotheca. The ovary,
oviducts and embryos are fixed in Stieve and were colored with H-
E, TB pH5,6; AB pH 2,5 and 0,5 combined with PA-Schiff. In the
ovary are oocytes in differents states. The stroma is abundant with
dense connective tissues, collagen fibers and follicles in different
stages of vitelogenic development; vascular-nervous packages and
thecals. Between the oocytes are MEC of proteics with some lipidic
vacuoles and oocytes in degeneration. Beside that we can find
proteins liquidicated component. It was observed only one atresic
follicle and a lutheum corpora. Folds with metacromatic-globed
cells form the oviducts with sulphated groups. Under the epithelium
we can find a dense and highly vascularizated connective tissue
with a few mucosae glands. The incubatriz pouch is
vascularizated thin skin fold with a stratificated epithelium and
mucosae glands. The embryos are in different developments stages.
It was observed; notochord, cartilage, muscles and some organs of
the digestive. Also we can observe great quantity of yolk in order to
achieve the embryos development to a mature stage; because this
specie has a direct development they will born as juvenile individuals.
Conclutions: There are a few oocytes in the pouch. Ovaries and
oviducts are different to species with other ovipositions modes.
BIOCELL 30(1), 2006
35.
INHIBITION OF PATHOGENS ASSOCIATED WITH
RED-LEG SYNDROME BY Enterococcus faecium STRAINS
Pasteris SE 1,2 , Roig Babot G 1 , Bühler MI 1 , Nader-Macías ME 2 .
1 INSIBIO-UNT and 2 CERELA-CONICET. Chacabuco 461. C.P.
4000. Tucumán. E-mail: pasteris@fbqf.unt.edu.ar
The commercial trade of Rana catesbeiana could be influenced with
epizootic such as red-leg syndrome (RLS) which causes mortality in
hatcheries. The utilization of probiotics represents an alternative to the
use of antibiotics and vaccines. In previous works we studied the beneficial
properties of Lactobacillus isolated from different areas of a hatchery.
The aim of this work was to study the inhibitory properties of Enterococcus
faecium strains against pathogens related with RLS: Proteus
vulgaris, Pseudomonas aeruginosa, Citrobacter freundii. Listeria
monocytogenes. and Salmonella enteritidis were included as meat spoilage.
Inhibitory properties were performed by the well difusion method
by using supernatants of overnigth Enterococcus cultures at 37°C. E.
faecium MCH 18a and MCH 22c isolated in september from healthy
animals inhibited all pathogens associated with RLS and L.
monocytogenes while one strain isolated from non-healthy animals inhibited
Ps. aeruginosa, pattern observed also in a strain from freswater
samples. From 27 strains isolated in december, only E. faecium GST 38
(isolated from balanced feed) inhibited RLS-related pathogens and L.
monocytogenes. The lost of inhibition observed after neutralization of
the supernatants as well as the low pH values indicated that the inhibition
was produced by acidity. However, L. monocytogenes was also inhibited
by hydrogen peroxide. In these work we select a group of E.
faecium strains to study other beneficial properties in order to design a
probiotic for aquaculture.
36.
NEUTROPHILS WITH TOXIC GRANULATIONS IN
NORMAL PREGNANT WOMAN
Lazarte S, Leri M, Agüero G.
Instituto de Bioquímica Aplicada, FBQF, UNT. Balcarce 747. San
M. de Tucumán. E-mail: gaguero@unt.edu.ar
The toxic or coarser granulation (TG) is the word that describe the
increase of density and possibly in the number of primary granulations
of neutrophils, that becames a great staining with the Giemsa
reactive. They can appear in infections, inflammation or intoxication.
Aim: to study the presence of TG in normal pregnant woman
in different periods of gestation. We realized micro-hematocrit determination,
the total an differential count of leukocytes (40x) and
the search of TG by optic microscope (100x), in 50 pregnant patients.
Three groups of 10 patients were chosen in aleatory way,
corresponding to 1°, 2° y 3° trimester of pregnancy, with an average
of age from 20 to 42 years old. Results:Hto (%):T1°=39,8±3,9;
2°=37,6±3,8; 3°=39,1±4.4. Leukocytes (10 9 /l):T1º=7,68±1,1;
2°=9,35±2,07 y 3°=10,42±2,26.
Neutrophil(%): T1°=68±4 ; 2°=68%±9 ; 3°=69±9. TG:4/10, , 6/10
y 7/10 pregnant woman of 1°,2° and 3° trimester respectively, presented
TG. It could see an important association (p