XXII Annual Scientific Meeting, Tucuman Biology Society ...

204 ABSTRACTS
153.
DETECTION OF VIABLE NON-CULTURABE Vibrio cholerae
O1 (NCVC) FROM RIVERS IN TUCUMAN, ARGENTINA
Silva C, Aulet O, De Allori CG, Cangemi R, Cecilia M.
Bacteriol., Fac Bqca,Qca,Fcia y Biotec., UNT. CP:4000.
Bacteria can use a variety of adaptive strategies to survive in the
environment. The temperature, light, evaporation and plankton can
affect the survival of microorganisms regarding their persistence
or ability to cause diseases. V.cholerae belongs to this group of
bacteria. In Tucumán the bacterium was sporadically isolated associated
with diarrhea. Due to its capacity to colonize sweet water
the objective of this study was to detect V. cholerae (NCVC) using
immunofluorescence (IF) to find environmental reservoirs, assessing
rivers in Tucumán. Three preestablished spots of the rivers Lules
(1) and Salí [Banda (2) and north canal (3)] were sampled during
the four seasons. The filter membranes for water were suspended
in 8 ml of PBS. To 1 ml of the PBS suspension and 1 ml of phyto
and zooplankton 100 μl of ANY medium was added and the mixtures
were incubated at 37°C for 6h. Then 4% formaldehyde was
added. Detection of Vibrio O1 NCVC using IF DFA-DVC (New
Horizons Diagnostics Lab, Columbia, MD, USA) Samples were
fixed on slides and reagent was added. Were incubated in a humid
chamber at 37°C for 30 min without light. Then they were washed
with PBS and examined. Vibrio O1 NCVC was detected in all the
samples with highest numbers in summer. It is necessary preventative
measures to avoid transformation of the rivers into a source of
infection.
154.
MICROBIOLOGICAL CONTROL OF TEA FROM SUPER-
MARKET SHELVES
Porcel N, Castillo M.
Cátedra de Bacteriología. Fac. de Bioq, Qca y Fcia. UNT. Ayacucho
491. CP: 4000.
Tea is a pleasant, popular, economical, safe and socially accepted
beverage, which is consumed daily by millions of people around
the world. It is obtained through an infusion of leaves from the
Camellia sinensis plant. This study make an assessment of the microbiological
quality of tea commercialized in S. M de Tuc, Arg, in
2004. Ten different tea brands, 5 coming in teabags and 5 in leaves,
were analyzed on the following: Total Mesophilic Aerobic Bacteria
(TMAB) in nutritious agar medium, Fungi and Yeasts (F+Y) in
fungus and yeast medium supplemented with chloramphenicol,
Total Coliform Bacteria at 37°C (C37) in Mac Conkey broth (most
probable number technique; MPN/100 ml), Fecal Coliform Bacteria
at 45°C (C45) in brilliant green broth, Presence or Absence of
Escherichia coli (EC) and Salmonella and Shigella (S/Sh) in lactose
broth, selenite and tetrathionate Salmonella Agar. TMAB figures
were between 2x10 2 and 2x10 4 cfu/ml. 4 samples showed values
for C37 within standard limits, whereas the 6 remaining were
above standard. Fungi varied between 2x10 and 3x10 3 and yeasts
between 5x10 and 3.1x10 3 . C45, EC and S/Sh were not detected.
Although most microbiological parameters of the commercial teas
were generally acceptable, presence of C37 in 60% of the cases is
a potential risk of appearance of pathogens. Consequently, periodic
controls should be intensified, so as to guarantee and assure
good quality manufacturing.
BIOCELL 30(1), 2006
155.
INDUCTION OF AN EXPERIMENTAL MODEL OF AU-
TOIMMUNE DISEASE BY INOCULATION OF THE RNP
ANTIGEN
Haro MI, Valdéz JC, Valverde M, Marsiglia R.
Histology Department, Faculty of Medicine, Universidad Nacional
de Tucumán. E-mail: mariaisabelharo@yahoo.com.ar
Mixed connective tissue disease (MCTD) is an illness of autoimmune
etiology with overlapping signs and symptoms of lupus,
dermatomyositis, rheumatoid arthritis and scleroderma and is associated
with anti-RNP antibodies. Objective: to induce an experimental
model for MCTD by inoculation with ribonuclearprotein
(RNP) antigen. Materials and Methods: 18 BALB/c mice of 40
weeks were inoculated as followed: group I) was injected with
100μg of RNP SC in complete Freund’s adjuvant and then weekly
with 50 μg in incomplete adjuvant until completing four inoculations;
group II) received 50 μg of RNP IP in complete Freund’s
adjuvant, continuing weekly with 50 μg until. Control mice were
inoculated with Freund’s adjuvant. Results: 8/18 mice shown visceral
hypertrophy; 4/18 alopecia; 2/18 corneal opacity; 2/18 malnutrition;
4/18 arthritis. There were no differences in function of
sex or inoculation route. No control mice shown signs or symptoms
of MCTD. Conclusion: our findings in inoculated mice with
RNP in Freund’s adjuvant were consistent with clinical features
described in patients with MCTD.
156.
HISTOPATHOLOGICAL FINDINGS IN LUNG OF AN
EXPERIMENTAL MODEL OF AUTOIMMUNE DISEASE
Valverde M, Uñates J, Alabarse G, Núñez JM, Carmona L, Haro MI.
Histology Department, Faculty of Medicine, Univ. Nacional de
Tucumán. E-mail: martahvb@hotmail.com
Mixed connective tissue disease (MCTD) was considered a pathology
of benign evolution but the clinical manifestations are now
well-known where the histopathological changes are wide and diverse.
Objective: to describe the histopathological findings in lung
using an experimental model of MCTD. Material and Methods: 18
BALB/c mice, of both sexes and 40 weeks of age were inoculated
with ribonuclearprotein (RNP) in Freund’s adjuvant (9 mice IP and
9 SC) in four doses, according to established protocol. Control
group: mice were only injected with Freund’s adjuvant. After the
third month of last inoculation mice were sacrificed and the organs
were processed (H-E and Gallego’s staining). Results: the most
characteristic findings were: lymphoid interstitial pneumonitis, interstitial
fibrosing pneumonitis and granulomas in subpleural area.
Conclusions: the histopathological findings in the lung of the mice
inoculated with the RNP antigen would be similar to lesions described
in patients with MCTD.