XXII Annual Scientific Meeting, Tucuman Biology Society ...

196 ABSTRACTS
121.
ISOLATION OF Candida SPECIES FROM DIFFERENT
NICHES OF ORAL CAVITY IN PERIODONTAL PATIENTS,
AND ITS RELATIONSHIP WITH SYSTEMIC CONDITIONS
López Rubio AC 1 , Garbero I 2 , Testa MM 1 , Cárdenas IL 1 .
1 Microbiology Department. 2 Preventive Dentistry. Faculty of
Dentistry. National University of Tucuman.
E-mail: carolopezrubio@hotmail.com
The aim of this work is to determine the prevalence of Candida
species in different ecological niches of the mouth: periodontal
pockets, saliva and oral mucosa, in periodontitis patients with and
without systemic diseases. Thirthy-three periodontitis patients between
19 and 76 years old were studied. Samples used for
micological diagnosis were: subgingival plaque, mucosa of palate,
cheek and tongue, and saliva. They were seeded in CRHOMagar
for colony counts and species identification. From the total of patients,
4 had diabetes, 1 had a cardiovascular disease, 4 were hypertensive,
and 7 were smokers. Candida was isolated from tongue
in 45.5% of patients, from saliva in 39.4%, from cheek mucosa in
24.2%, from palate in 21.2% and from subgingival plaque in 18.2%.
C. albicans was the most prevalent species in all ecological niches
and the only species identified in periodontal pockets. C. krusei was
isolated from saliva, tongue and palate; C. tropicalis in saliva and
tongue, and C. parapsilosis only in tongue. Significant statistical
differences (p= 0.004) between smokers and no smokers related with
Candida isolation from saliva. There were not significant statistical
differences between having or not other systemic diseases, except
when diabetic condition and smoking were considered together
(p=0.01). Tongue is the ecological niche with higher diversity of
Candida species, being the most prevalent C. albicans, and it was
the only species isolated form periodontal pockets. Diabetic patients
who are smokers had more risk to be colonized by yeast.
Supported by CIUNT.
122.
KNOWLEDGE OF GRADE AND POST GRADE DENTISTRY
STUDENTS OF DENTAL ATTENTION OF PREGNANT
WOMEN
Garbero I 1 , López Rubio AC 2 , Navarro A 1 , Gutiérrez de Ferro MI 2 ,
Cárdenas IL 2 .
1 Microbiology Department. 2 Preventive Dentistry. Faculty of
Dentistry. National University of Tucuman.
E-mail: carolopezrubio@hotmail.com
The aim of this work was to evaluate grade and postgrade students’
knowledge about dental practices in pregnant women. A qualitative-quantitative
research was realized using an auto-administered
questionnaire in a group of 28 dentistry students and 14 residents.
The questionnaire considered: knowledge of the clinical pregnancy
history, derivation to dental control during pregnancy control (PNC),
aspect of mouth health during the pregnancy, dental treatments that
can be realized without risk for the fetus, best moments for clinical
therapy, etc. Mean age of students was 24.7 ± 2.7 years old, 6 were
males and 36 women. 69% does not know the clinical perinatal
history, 59.5% knows derivation norms for dental surveillance during
PNC, and 73.8% thought that written authorization of physician
was need for any dental practice. 71.4% of the students considered
gingival status as the main problem. About the main reason
of dental complications, 90.5% of the students considered poor oral
hygiene, 81% hormonal changes in pregnancy and 54.8% vomits
of the first quarter. 88.1% considered that basic periodontal therapy
does not represent risk for the fetus, and 47.6% believed that the
second quarter was better for therapyThere were no significant
differences in knowledges between grade and post-grade students.
Knowledge demonstrated by dentistry students and residents about
dental practices during pregnancy were insufficient.
Supported by CIUNT.
BIOCELL 30(1), 2006
123.
RELATIONSHIP AMONG Candida SPECIES ISOLATED
FROM ORAL CAVITY WITH CARIES AND PERIODONTAL
DISEASE
Lopez Rubio AC 1 , Garbero I 2 , Testa MM 1 , Cardenas IL 1 .
1 Microbiology Department. 2 Preventive Dentistry. Faculty of
Dentistry. National University of Tucuman.
E-mail: carolopezrubio@hotmail.com
It has been suggested that the use of broad spectrum antibiotics
can promote superinfections with yeast in periodontal pockets.
Candida albicans is considered among dental caries associated
microorganisms. The aim of this work was to establish the relationship
among Candida isolation and caries and periodontal disease,
and to describe species distribution in both groups. Thirtythree
patients of the Faculty of Dentistry, with periodontal disease
(PD) and the same number with dental caries (C) were selected. A
clinical examination recording the following parameters were done:
sugar intake, plaque index and CPOD (number of teeth with caries,
lost and obturated). At the same time saliva simples for micological
studies were collected. Samples were seeded in CRHOMagar for
colony counts and species identification. Plaque Index mean was:
1.37 ± 0.67 (C) and 1.66 ± 0.60 (PD), (p>0,05). Saliva CFU/ml
mean were: 481.33 ± 962.45 (C) and 249.70 ± 896.64 (PD) (p>0,05).
In the 66.7% (C) and 63.6% (PD) yeast species was not identified.
24.2% (C) and 30.3% (PD) were C. albicans and 3% of each group
had C. tropicalis. C. krusei were isolated in only one periodontitis
patient and C. parapsilosis in two caries patients. There was not
difference in Candida isolation between the two oral diseases. C.
albicans was the species most frequently isolated in the two groups.
Supported by CIUNT.
124.
RELATIONSHIP BETWEEN PROSTAGLANDIN E 2 AND
ANTIGEN PRESENTING CELLS IN AN ANTITUMORAL
IMMUNOTHERAPY EXPERIMENTAL MODEL
Valdez JC, Gobbato N, Mercau GT, Huaman M, Mercau G.
Facultad de Medicina, Cátedra de Histología. Av. Roca 2100,
(4000) Tucumán. E-mail: pumavaldez@hotmail.com
Intrroduction: Dendritic cells (DC) are of transcendental significance
in the induction of antitumoral response. Prostaglandin E2
(PGE 2 ), and chemotherapic agents can inhibit the immune response.
We analyze the relationship between splenic and plasmatic PGE 2 ,
with the generation of splenic DC in the context of mice breast
cancer treated with chemotherapy and immunostimulation.
Material and methods: 12 Balb/c mice with M3 breast cancer
where separated in four groups: Mice in Group D were inoculated
with IV doxorubicin (D) 0.2 ml (150 ug /ml); Group V received 0,1
ml of IM Ribovac Vaccine (2,5 ug of bacterian RNA + 280 mg of
proteoglycans/ml); group DV received D and after 24 h Ribovac
Vaccine , while group T served as control. PGE 2 concentration and
the presence of DC were determined.
Results: The concentration of PGE 2 was significantly higher in
group D (460±57 pg/ml) and D+V (480±67 pg/ml) p < 0,05. The
percentage of DC was also higher in group V (28±2,9) when compared
with T (18±2,2 p