XXII Annual Scientific Meeting, Tucuman Biology Society ...

176 ABSTRACTS
41.
INCREASE OF ANTIOXIDANT ENZIMES IN THE
PLACENTA OF STRESSED RATS
Romanini MC 1 , Paz DA 3 , González E 2 , Soñez CA 1 , Mugnaini MT 1 ,
Rolando A 1 , Bozzo A 1 , Pastorino I 1 , Gauna HF 1 .
1 UNRC. 2 CEFyBO. 3 UBA. E-mail: cromanini@ayv.unrc.edu.ar
The placenta is a fundamental organ to maintain the homeostasis
of the maternal-fetal phase, and it is able to reflect any anomaly in
the maternal environment, for example, an increment in the stress.
The maternal stress is translated in an alteration in the profile oxidative
placentary. Reactivate oxygen species (ROS), (hydrogen
peroxide, superoxide anions and hydroxil radicals) are oxidants of
molecules that integrate the cells, damaging them. Superoxide
Dismutase (SOD), eliminates the superoxide anions and Catalase
(CAT) descompose the hydrogen peroxide. Our objective was to
evaluate the impact of the maternal stress on the activity of SOD
and CAT under controlled conditions of laboratory in placentas of
controls (C) and stressed chronically (S) Wistar rats of 12, 17 and
21 days of pregnancy. The chronic stress was achieved by immobilization
during 45 min. /3 times per week. The enzymatic activities
of SOD (Yamanaka’s technique) and of CAT(Kankofer’s technique)
were determined in placentary homogenates. Our results show that
SOD and CAT were increased in 17 and 21 days of pregnancy in S
rats with statistical significant differences (p