XXII Annual Scientific Meeting, Tucuman Biology Society ...

210 ABSTRACTS
177.
COOPER ACCUMULATION IN ROOTS, STEMS AND
LEAVES OF A FOOD PLANT
Benimeli CS 2 , Medina A 2 , Navarro CM 1 , Medina RB 2 , Amoroso MJ 2 ,
Gomez MI 1 .
1 Cát. Qca. and 2 Qca. Biológica. Fac. Cs. de la Salud. UNSTA. 9 de
Julio 169. (4000) S M Tucumán. E-mail: minesgomez@arnet.com.ar
Plants metal biosorption are affected by species, growth and chemical
elements characteristics. In Tucumán there is a copper filter
plant that produces metal contamination in agriculture surrounding
areas. The aim of this work was to determine Cu accumulation
in maize plant and its capacity of growth. Maize seeds were germinated
in sterile, humid and dark conditions at 30 °C for 3 days,
being later seeded in an inert material. The plants were watered 10
days with nutritive broth, 6 more with also CuSO 4 250 mg/L and
kept at 30 °C with light cycles of 12 h. The growth was evaluated
in roots, stems and leaves. Cu accumulation was measured by atomic
absorption spectrophotometer. A growth diminution of 10% in roots
and leaves was observed compared to the controls. Only steams
and leaves presented 16 and 13% weights reduction. Steams Cu
accumulation was 93% more than the controls, however in roots
and leaves were 83 and 86%. The capacity of Cu(II) accumulation
by a food plant, could be prejudicial for the trophic chain because
their toxicity in high concentrations
178.
ELICITATION OF DEFENSE REACTIONS IN
STRAWBERRY (Fragaria x ananassa) BY ELICITOR
DERIVED FROM Colletotrichum
Chalfoun NR, Arias ME, Salazar SM, Castagnaro AP, Díaz Ricci JC.
INSIBIO (CONICET-UNT). Chacabuco 461. CP 4000. Sección
Biotecnología de la EEAOC. Tucumán.
E-mail: nadiarchalfoun@hotmail.com
The oxidative burst generating reactive oxygen species (ROS: H O , 2 2
- O ) is one of the earliest plant responses activated by a defense
2
elicitor. Accumulation of H O can trigger cell wall thickening, in-
2 2
duction of defense genes and a localized cell death called hypersensitive
response (HR). The aim of the study was to analyze the
occurrence of defense reactions in response to a fungal elicitor in
strawberry plants. The elicitor was obtained from culture filtrate of
an avirulent strain of Colletotrichum fragariae and infiltrated on
the abaxial surface of two leaves per plant of the cultivar Pájaro.
Histochemical localization of H O showed spread micro-bursts in
2 2
treated leaves with a maximum accumulation four hours after infiltration.
In the same areas we have also detected the reinforcement
of mesophyll cell wall due to the apposition of lignin and the
swelling of few adaxial epidermal cells followed by their collapse
24 hours latter. HR cell death was visualized at infiltration sites by
yellow autofluorescence under UV-light (310 nm) excitation indicating
the accumulation of polyphenols. Our results indicate that
the H O burst is closely associated with HR and the lignification.
2 2
These local defense responses induced a systemic protection when
plants were challenged with a virulent pathogen.
BIOCELL 30(1), 2006
179.
ANALYSIS OF TISSUE TYPE PLASMINOGEN ACTIVATOR
(t-PA) IN PORCINE OVIDUCT
Roldán-Olarte M, Valdecantos P, Miceli DC.
Instituto de Biología. Facultad de Bioquímica, Química y Farmacia.
INSIBIO. UNT. Chacabuco 461. 4000. San Miguel de Tucumán. Email:
emroldanolarte@fbqf.unt.edu.ar
Plasminogen activators are highly specific serine-proteases that
convert plasminogen to plasmin, an active protease with a very
broad spectrum of substrates. In previous works, we determined
the activity of plasminogen activators and the expression of urokinase
type plasminogen activator (u-PA) in the epithelium of the
porcine oviduct. The aim of this work was to demonstrate that tissue
type plasminogen activator (t-PA) is synthesized and secreted
in the porcine oviduct as well as to study the differences between
the phases of the estrous cycle. Western Blot assays performed with
oviductal fluid obtained in the follicular and luteal phases, by using
a monoclonal antibody against t-PA, showed a band of 72 kDa
corresponding to MW reported for porcine t-PA. The presence of t-
PA was observed in both phases of the estrous cycle. The t-PA concentration
was higher in samples obtained during follicular phase
than luteal phase. The expression of t-PA gene studied by semiquantitative
RT-PCR in both ampulla and isthmus oviductal regions
indicated no significant differences between both segments. When
we studied the t-PA gene in the epithelial cells, an up-regulated
expression during the follicular phase in this tissue was observed.
According to these results, the presence of t-PA in epithelial cells
was also confirmed by immunohistochemical assays. This work
indicates that the t-PA is synthesized in the porcine oviduct and
secreted to the oviductal lumen, where it could activate plasminogen
to plasmin near gametes or embryos. This enzyme could act
directly or indirectly on the extracellular matrix (ECM) components
in the last case by activating matrix metalloproteases or grow
factors present in the ECM.
180.
REACTIVATES SEROLOGY FOR TRANSMISSIBLE
DISEASES BY TRANSFUSIONS IN DONORS OF THE
PROVINCE OF TUCUMÁN AT OFFICIAL LEVEL. PERIOD
2004
Arenas Hernández M de los A, De Rosa R, Guzmán A del V, Guzmán
MJ, Martorell Ortiz M del C, Gramajo Antuz GE.
Av. Avellaneda 750. SM de Tucumán, Tucumán. CP 4000.
Chacabuco 525. E-mail: rodrigoderosa@hotmail.com
Descriptive, transversal cohort study, over 12,981 donors from
Tucumán official data base (Argentina) Period 2004. Objectives:
Describe the frequency of reactive serology for transmissible disease
by transfusions (TDT) in blood donors and discriminate it
according to the type of disease. The information was obtained of
the Tucumán official data base. Exclusion Criteria: Samples without
pertinent studies. Descriptive study of variables was made.
Reactive serology presented in 1567(12%) on 12,981 donors. The
most frequently diseases founded were: Chagas 7%(862 cases), anti-
HBcore 4%(454), anti-HCV 1,4%(185), HBsAg, 1%(129), the rest
was presented in less of 0,4%. Comparing the frequency founded
(12%) with studies that used normatized systems of tamizaje (4%)
was observated a greater significantly difference. The differences
with the studies descripted would indicate that it would be necessary
to fit the tamizaje methods.