XXII Annual Scientific Meeting, Tucuman Biology Society ...

198 ABSTRACTS
129.
CONTENT OF POLYPHENOLS IN LEMON JUICE FROM
TUCUMAN
Alvarez A, Jorrat S, Genta M.
INTA EEA Famaillá, Ruta 301 km 32, Tucumán y Cát. de Qca.
Analítica II, FaCET, UNT, Avda. Roca 1800. Tucumán.
The content of total polyphenols is a parameter that allows to characterize
the lemon juice from a certain geographical region and
also to contribute information about a nutritional component that
has possitive effects on the human health, since there has been demonstrated
that the polyphenols reduce the risk of cardiovascular
diseases. The objective of the present work was to evaluate the
content of total polyphenols in lemon juice from Tucumán as a
parameter of characterization and to compare it with other drinks
reported in the bibliography as source of polyphenols, such as red
wine and black tea. There were analyzed 47 samples of concentrated
juice of lemon, representative each one of a batch of 10000
liters of concentrated juice. The concentrated juice was diluted up
to reaching the brix corresponding to a natural juice. The determination
of total polyphenols was realized for Folin Ciocalteu’s
method. The average content of total polyphenols was of (933.0 ±
11.3) mg of Gallic acid equivalent for liter. This concentration is
four times higher than the content of plyphenols of red wine (210
mg/L) and black tea (235 mg/L). In conclusion, the lemon juice
from Tucuman has a high content of total polyphenols.
130.
RADIOGRAPHIC STUDY OF MIXED DENTITION
ACCORDING TO NUTRITIONAL STATUS
Romero S 1 , Páez R 2 , Lencina V 3 , Lorca A 1 , Delgado AM 2,4 .
1 Sala de Rayos, 2 Odont. Preventiva, 4 Farmacología y Terap. Fac.
de Odontología. 3 Bioestadística, Fac. de Medicina. UNT.
Objective: To determine radiographically on size, shape, number
and mineralization stage of primary and permanent dentition on
normal and malnourished children from S.M. de Tucumán Material
and Metods: Two groups were formed: one uetrofic children
and other malnourished. Retroalveoar radioghaph from left lower
arch were taken. Dental age was determine by the dental developmental
stage adapted by Haavikko. Results: Sample was constituted
by 34 malnourished and 55 normal. Proportion of children
with studied alterations, didn‘t vary according to nutritional status.
(P>0.15 in all pathologies). It was detected though, that 1º permanent
molar eruption grade differ significativelly according to child
nutritional status (P=0.023), non erupted 1º molar proportion is
higher in malnourished children and root calcification degree differ
according to nutrition status (P=0.01). Proportion of children
with deficient radicular development degree (< a 1/3) is higher
between malnourished than normal. Conclusion: studied pathologies
don‘t differ according to nutritional state. Among malnourished
1º molar eruption degree and radicular development is lower
than that in normal children.
BIOCELL 30(1), 2006
131.
ISOLATION OF A PROTEIN DERIVED FROM SPERM
EXTRACTS CAPABLE OF INDUCING THE ACTIVATION
OF Bufo arenarum OOCYTES MATURED IN VITRO
Bonilla F, Ajmat MT, Sánchez Toranzo G, Zelarayán L, Oterino J,
Bühler MI.
Instituto de Biología. UNT - INSIBIO. Chacabuco 461. (4000)
Tucumán. E-mail: mbuhler@unt.edu.ar / mbuhler@unt.edu.ar
In the fertilization of most animals, egg activation is accompanied
by an increase in cytoplasmatic Ca++; however, the mechanism
through which the fertilizing sperm induce this phenomenon is still
controversial. This work aims at: a) the isolation of the active fraction
of the sperm extract; b) the analysis of its effect on egg activation
under different experimental conditions.
The sperm extract was subjected to gel filtration chromatography
and the fraction with biological activity was dialyzed against 1%
NaCl; then polyacrylamide-SDS electrophoresis was carried out.
The experiments were performed with in vitro denuded Bufo
arenarum oocytes. The activation parameters considered were the
disappearance of the white spot, the elevation of the vitelline envelope
and the cortical granules exocitosis.
The results indicate that in the fraction with biological activity of
the sperm extract there is a protein of approximately 30 Kda. This
protein is capable of activating the eggs in a dose-dependent manner
when it is added to the culture medium; however, it has no
effect when microinjected into the egg cytoplasm. These results
indicate that the protein isolated from the sperm extract would interact
with receptors on the oocyte membrane capable of activating
the signaling pathways that lead to activation.
132.
MICROBIOLOGICAL MONITORING OF SUGARCANE
JUICE AT DIFFERENT STAGES OF THE CLARIFICATION
PROCESS
Anduni G, Gusils C, Ruiz M, Cárdenas G.
EEAOC, Av. Williams Cross 3150. Tucumán, Argentina. E-mail:
guilleanduni@eeaoc.org.ar
Cane sugar manufacturing process begins with the reception of
sugarcane in the sugar mill, where it is crushed to extract its sugared
juice, which is subsequently filtered, sulfated, decanted, limed
and heated. The juice is then decanted in clarifiers to obtain clarified
juice. During all these stages, the microorganisms existing in
sugar cane play an important role in relation to the efficiency and
quality of the final product.
As a first step to control microbiologic quality, the microbial content
of sugarcane juices obtained in Tucumán sugar industry, was
analyzed. The microbiological methodology was performed according
to ICUMSA official protocols.
When analyzing the values obtained, it could be observed that in
the case of first expressed juice and mixed juice, most of sugar
mills exhibited very similar count of bacteria, fungi, yeast or
polysaccharide-producing microorganisms.
In clarified juices, values obtained were low, and fungus and yeast
were absent.
From the results mentioned above, it can be stated that it is important
to control different aspects of the clarification process in order
to avoid the appearance of undesired microorganisms affecting yield
efficiency.