XXII Annual Scientific Meeting, Tucuman Biology Society ...

188 ABSTRACTS
89.
ANTIMICROBIAL EFFECT OF XENOPHYLLUM POPOSUM
ON ACTINOMYCES NAESLUNDII
Torres S, Tracanna MI, Amani S, Poch M, Cárdenas L, Ferro M,
Gutiérrez S.
Microbiology Dept., Fac. of Dentistry. Farmacognosy Dept.,
Bioqca, Fac. UNT. Av. Benjamín Araoz 800. C. P: 4000. Tucumán.
E-mail: sofiatorresar@yahoo.com.ar
The control of dental caries involves the mechanic elimination of
dental plaque with the use of antimicrobial agents. The inhibition
of Actinomyces naeslundii, associated to this disease, due to
ethanolic extract of Xenophyllum poposum was demonstrated by
our laboratory. The aim of this work was to determine the Minimum
Inhibitory Concentration (MIC) and Minimum Bactericide
Concentration (MBC) of the ethanolic extract of X.poposum on
A.naeslundii. Dilutions of 1%, 5%, 10% and 20% of extract were
assayed. DO 560 nm at 0, 24 and 72 h was determined and the count of
colonies in Thioglycolate Agar was made to determine MBC. All
the tests were made by duplicate.
Results:1) The MIC of the extract in study was 10%. 2) The MBC
was 10%.
Conclusions: The determination of MIC and MBC of the ethanolic
extract in study is important to control of A. naeslundii. Alternative
therapies must keep in mind the ecological aspects of oral cavity
to maintain the balance of the total microbiota in mouth.
Subsidized by the Research Council of UNT and Laboratory NAF
90.
EVALUATION OF INFECTION CONTROL IN STUDENTS
OF FAC. OF DENTISTRY. UNT
Granillo BA, Annan MS, Benito de Cárdenas IL.
Microbiology Dept. Fac. Of Dentistry. UNT. Benjamín Araoz 800.
San Miguel de Tucumán. E-mail: granillo@arnet.com.ar
It is necessary to standardize the preventive measures inside the
Program of Infection Control in Faculty of Dentistry. The objective
of this work was to evaluate through questionnaires to students
of Fac. of Dentistry-UNT, on knowledge and application of
the norms of infection control.
It was compared to students’ groups according to time of coursed in
Microbiology. A multiple and anonymous survey of questions was
designed. Results: 53% is immunized for HVB; 93% uses physical
barriers; 86% make a correct sterilization; 67% recognizes the importance
of the handwashing; 87% assumes that the all healthcare is
responsible in the prevention of cross-infections. We empathize the
percentage of negative answers: 73% doesn’t carry out disinfection
of surfaces; 76% doesn’t purge the pipes of the dental water lines;
and 50% not know the measures in postexposure accidents.
Conclusions: The students know the risks to those that are exposed
in the clinic. It should be carried out monitoring and surveillance
in the clinical practices, immunization for HVB and to insist in the
Prevention Postexposure.
Supported by CIUNT.
BIOCELL 30(1), 2006
91.
COMPARATIVE STUDY BETWEEN PLANT EXTRACTS
(CAESALPINIA PARAGUARIENSIS) WITH ANTIBACTE-
RIAL ACTIVITY AND SYNTHETIC ANTIBIOTICS OF
FREQUENT APPLICATION IN ANTIINFECTION THERAPY
Sgariglia MA, Soberón JR, Quiroga EN, Vattuone MA.
Cátedra de Fitoquímica. Instituto de Estudios Vegetales “Dr. Antonio
R. Sampietro”. Facultad de Bioquímica, Química y Farmacia.
UNT. Ayacucho 471. (4000) S.M. de Tucumán. Argentina. E-mail:
instveg@unt.edu.ar
The aim of this study was the evaluation of the in vitro antibacterial
activity of Caesalpinia paraguariensis extracts (Infusion, Decoction
and Tincture) against 22 bacteria. This activity was compared
with that of synthetic antibiotics. Synthetic drugs: Amoxicillin
and Ciprofloxacin, were chosen taking into consideration their antibacterial
spectrum, action mode and mechanisms, theirs frequency
of use as therapeutic indication. Streptomycin sulphate, an ample
spectrum antibiotic, was employed to compare the plant pathogenic
action. We determined MICs and MBCs by broth microdilution
method, and agar dilution method respectively (Muller Hinton
medium). Although plant extracts and synthetic drug MICs and
MBCs are not comparable from a quantitative point of view, some
strains were sensible to the assayed extracts and simultaneously
resistant to synthetic antibiotics. Moreover, we will use them as
reference in the bioactivity-guided purification process.
92.
ANTIBACTERIAL ACTVITY OF CAESALPINIA
PARAGUARIENSIS EXTRACTS
Sgariglia MA, Soberón JR, Quiroga EN, Vattuone MA.
Cátedra de Fitoquímica. Instituto de Estudios Vegetales “Dr. Antonio
R. Sampietro”. Facultad de Bioquímica, Química y Farmacia.
UNT. Ayacucho 471. (4000) S. M. de Tucumán. Argentina. E-mail:
instveg@unt.edu.ar
The ethnomedicinal use of plants reveals their importance as source
of bioactive compounds and orients us toward the search of new
substances with potential therapeutic applications. The objective
of this study was to validate the antibacterial (AB) activity of extracts
of a native species of Argentinean North, used in popular
medicine as vulnerary. Guayacán (Fabaceae) bark extracts (Infusion
(I), Decoction (D) and Tincture (T)), according Argentine Pharmacopoeia
VI Ed., were assayed against 22 bacteria: Gram (+) and
Gram (-) isolated from superficial wounds, ATCC and plant pathogenic
bacteria (CECT). Broth microdilution and agar macrodilution
techniques were employed in Muller Hinton (MH) medium. MIC
and MBC values were determined. All tests were performed by
quadruplicate. The extracts have a wide antibacterial activity, showing
an evident growth inhibition and bacterial death on all the assayed
strains. MIC values of I and D were 20-300 and 24,9-398,7
μg. Extracted Material / 100 μl, respectively, that are higher than T
AB activity (20-650). MBC values were coincident or higher than
MIC values.