3. Postere - rmr.medica.ro

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<strong>3.</strong>
ence, fasting plasma glucose and HbA1c. As
we expected, the body and trunk adipose tissue
percents were higher in the group 2, but
without statistical signifi cance.
Fasting insulin and HOMA IR were positive-
ly correlated with waist circumference, body
mass index and body fat percent.
Study on the paraoxonases: repercussions
for chronic metabolic diseases and
atherosclerosis risk
Daniela Lixandru 1,2 , Irina Stoian 1 ,
Bogdana Virgolici 1 , E.V. Bacanu 3 , Maria Mohora 1 ,
Constanti n Ionescu-Tirgoviste 3
1 Department of Biochemistry,
University of Medicine and Pharmacy “Carol Davila”,
Bucharest, Romania
2 Insti tute of Biochemistry of the Romanian Academy,
Bucharest, Romania
3“ N Paulescu” Nati onal Insti tute of Diabetes, Nutriti on
and Metabolic Disease, Bucharest, Romania
One of the strongest risk factors for coronary
heart disease (CHD) has proved to be low plasma
high-density lipoprotein (HDL) concentration.
HDL is subject to substantial compositional variations
under both normal and pathological metabolic
conditions.
The antioxidant activity of HDL is largely due
to the paraoxonase1 (PON1) located on it. Studies
in the last two decades have demonstrated PON1
ability to protect against atherosclerosis by hydrolyzing
specifi c derivatives of oxidized cholesterol
and/or phospholipids in oxidized low-density lipoprotein
and in atherosclerotic lesions. Signifi cant
advances have been made in understanding the basic
biochemical function of PON1 and the discov-
REVISTA MEDICALÅ ROMÂNÅ – VOLUMUL LIX, NR. 2, An 2012
Sex HOMA-IR
Groups Women Men p
Group 1
HOMA-IR4
p
Age (years) 56.79±7.33 57.03±8.21 ns 58.51±5.92 56.47±7.30 ns
Weight (kg) 87.07±1<strong>3.</strong>16 96.58±11.12 ** 86.83±12.89 89.62±11.38 ns
BMI (kg/m2 ) 34.15±5.27 32.49 ±<strong>3.</strong>60 0.08 32.08±<strong>3.</strong>73 32.99±<strong>3.</strong>90 ns
WC (cm) 108.54±11.24 109.90±7.62 ns 104.27±9.50 108.47±9.76 ns
FPG (mg/dl) 152.63±41.87 130.79±37.51 0.02 130.65±3<strong>3.</strong>99 157.70±44.16 *
HbA1c (%) 6.85±1.30 6.81±1.10 ns 6.46±1.04 6.97±1.37 0.09
Fasti ng insulin (µU/ml) 15.59 ±8.32 12.40 ±5.16 * 8.11±2.84 18.24±6.47 **
HOMA-IR
index
5.90±<strong>3.</strong>44 4.15±2.12 * 2.62±0.96 6.97±2.91 **
Body adipose ti ssue
percent (%)
4<strong>3.</strong>48±4.64 29.62±5.71 ** 36.84±6.85 39.24±7.79 ns
Trunk adipose ti ssue
percent (%)
40.48±5.84 3<strong>3.</strong>71±4.45 ** 36.08±5.36 38.56±6.96 ns
ery of possible modulators of its activity. Recently
two other members of the PON gene family, namely,
PON2 and PON3 have also been reported to
possess antioxidant properties and may exhibit antiatherosclerotic
capacities as well. By this time is
well know that paraoxonases are enzymes with
three (paraoxonase, arylesterase and lactonase) activities
which are inversely related to cardiovascular
disease.
This study consists in the comparative analysis
of the PONs activity and the relevance for atherosclerotic
process. Is also present the implication of
environmental factors and oxidative stress as well
as the pozitiv corelation between PONs levels and
degrees of metabolic disorders associated with atherosclerosis
like obesity and diabetes mellitus.
Acknowledgement. Dr. D.L. was supported by
the postdoctoral program POSDRU/89/1.5/S/60746,
from European Social Fund.
Rolul stresului oxidati v în boala renală
diabeti că
Elena Violeta Băcanu 1 , Daniela Lixandru ²,
Irina Stoian 2 , Bogdana Vîrgolici 2 , Maria Mohora 2 ,
Constanti n Ionescu-Tîrgovişte 1
1 Insti tutul Naţional de Diabet, Nutriţie şi Boli
Metabolice ,,N.C. Paulescu“, Bucureşti , România
²Universitatea de Medicină şi Farmacie ,,Carol Davila“,
Catedra de Biochimie, Bucureşti , România
Introducere
Boala renală diabetică (BRD), defi nită ca o boală
glomerulară progresivă, de natură predominant infl
a matorie, evoluează negativ în mediul diabetic.
Scopul prezentului studiu a fost evaluarea statusului
oxidativ/antioxidativ şi corelarea acestuia cu mar-