Chromatotron

CHANGING ROTORS
Lift the retaining clips with a finger or thumb and slide back about 1 cm. The
Teflon lid can now be removed. Hold the shaft collar stationary when screwing
the endplate in or out. Alternatively and more conveniently, turn the shaft collar
and hold the endplate stationary.
1 Unscrew and remove endplate. → 2 Slide rotor up with finger → 3 Remove rotor.
and place shoehorn under
lower edge. ↓
6 Use endplate in center hole 5 Slide rotor up with finger 4 Lower new rotor
to slide rotor up into position. to allow removal of shoehorn. into position.
Screw in endplate by hand only. ← ←
A tight endplate can be levered loose with the endplate key. Insert into one of
the holes at the side of the endplate. See diagram page 3.
If the slack between the screw threads on the endplate and the hole in the rotor
causes slight eccentricity, loosen the endplate and screw up in a new position
relative to the rotor. Repeat until concentric. Eccentricity of the sorbent layer,
or the glass rotor, does not affect performance.
SORBENT LAYER THICKNESS
1 mm Layers. For separation of small samples, up to 100 mg per component,
250 mg total sample.
2 mm Layers. For larger sample loads, up to 300 mg per component, 750
mg total sample. Also for small samples of low solubility or which tend to tail.
The resolving powers of 1 and 2 mm layers are not significantly different for
light loadings.
4 mm Layers. For very large loads, up to about 1.5g. Gain experience with 1
and 2 mm layers before using these thick layers.
Layers up to a thickness of 8 mm can be prepared but are troublesome to use.
9