Chromatotron

_________________________________APPENDICES
APPENDIX 1. THE TEST MIXTURE
A mixture of high Rf colored compounds is useful for testing sorbent layers.
"The test mixture" refers to a mixture of the 2,4-dinitrophenylhydrazones of
cyclopentanone and cycloheptanone. The same derivatives of open-chain
ketones are also suitable. Azobenzene derivatives and the commercial dye
mixtures used with regular TLC plates are not recommended since they are
not completely stable and contain low Rf impurities that remain on the sorbent.
APPENDIX 2. INTRODUCING LARGER VOLUMES OF SAMPLES
Large volumes of sample solutions
can be introduced through a syringe
barrel clamped at the same height as
the Teflon funnel.(Suggested by
J.G. Krause, Niagara University).
A cotton plug filter is required
in the syringe barrel to prevent
clogging of the stainless steel
capillary tubing by solid impurities.
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