2009 Vienna - European Society of Human Genetics

Cytogenetics
P03.028
HLA B27 allele status in turkish patients with
spondyloartropathy
P. Ata Eren 1,2 , A. Aktaş 1 , N. Denizli 1 , F. Akdogan 1 , H. Sokmen 1 , S. Erdem 3 , M.
Solak 4,5 ;
1 Haydarpasa Numune Research and Training Hospital, Istanbul, Turkey, 2 Genetics
Diseases Research Center, Turkey, 3 Kirikkale Univ. Dept. of Medical Biology,
Ankara, Turkey, 4 Afyon Kocatepe Univ., Dept. of Medical Biology, Afyon,
Turkey, 5 Council of Higher Education of Republic of Turkey, Ankara, Turkey.
Some of the spondyloarthropathies are inflammatory joint diseases of
the vertebral column associated with the MHC class I molecule HLA-
B27. It has shown that HLA-B27 heavy chains can form stable homodimers
lacking ß2m, and it is possible that such an aberrant form of the
B27 molecule could be involved in disease pathogenesis
Two hundred and seventeen patients admitted to our center because
of artropathy and or uveitis symptoms between July 2008 and January
2009 were analysed for HLA B27 allele status. Patients were informed
and gave consent. Genomic DNA was isolated from peripheral
blood leukocytes by ethanol precipitation. HLA B27 allele status was
searched by sequence specific primer method by multiplex PCR covering
HLA B27 and B73 alleles. With the detection of the 145 and 95
base pair products along with internal control bands it was considered
as the presence of these alleles.
In this study 67% (145/217) of our patients were carrying B27 allele.
There were patients with uveitis (32/145), reactive artritis (25/147), inflammatory
bowel disease (18/147), spondyloartropathy (72/147). Patients
had neutrophilic type leukocytosis (43%), raised ESR (66%) and
anemia (45%). We determined female predominance in allele positive
group in contrast to the literature (p