2009 Vienna - European Society of Human Genetics

Genomics, Genomic technology and Epigenetics
Centa2 is also expressed in liver and pectoral fin bud. We observed a
weak signal in the bulbus arteriosus at 48-72 hpf, but not in the remaining
heart portions. Suz12 shows an intense hybridization signal in the
medial and rostral region of the embryo at 30-72 hpf, but no expression
in the heart. Considering that zebrafish heart has two chambers, we
speculate that Centa2 and Suz12 might be important for cardiac morphogenesis
in the most evoluted organisms, such as mammals, which
have a four-chambered heart.
P11.016
the regulation of cDK5R1 gene expression by miRNAs may
have a role in Alzheimer’s disease
S. Moncini 1 , M. Venturin 1 , A. Salvi 2 , P. Zuccotti 1 , V. Lanzi 1 , C. Sabelli 2 , G. De
Petro 2 , S. Barlati 2 , P. Riva 1 ;
1 Department of Biology and Genetics for Medical Sciences - University of Milan,
Milan, Italy, 2 Division of Biology and Genetics, Department of Biomedical Sciences
and Biotechnologies - University of Brescia, Brescia, Italy.
CDK5R1 encodes for p35, an activator of CDK5, which is involved in
neuronal migration and differentiation during CNS development and is
hyperactivated in Alzheimer’s disease (AD) leading to Tau hyperphosphorylation.
We recently reported that the large 3’UTR of CDK5R1
contains regulatory elements affecting transcript stability. Many microRNAs
(miRNAs) target sites have been predicted by PicTar software.
We evaluated the expression of nine pre-miRNAs, among the
20 miRNAs predicted to bind CDK5R1, in six cell lines. Among the expressed
miRNAs, we observed that five of them present a high number
of target sites with a free energy