2009 Vienna - European Society of Human Genetics

Statistical genetics, includes Mapping, linkage and association methods
TGF-β improve the efficiency of efferocytosis (engulfment of apoptotic
cells by phagocytes followed by cell replacement to maintain homeostasis)
in lung, and several lung diseases, including Asthma, Chronic
Obstructive Pulmonary Disease(COPD) and Cystic Fibrosis (CF) result
from impaired efferocytosis. In order to clarify the significance of
each of TGF- β isoforms in lung disease of people poisoned by toxic
inhalants, we examined the mRNA expression for TGF- β 1, TGF- β 2
and TGF- β 3 in lung biopsies of chemical injured patients, and compared
it with non-injured people. Lung samples were collected from
both groups using small pinchers attached to a long cable threaded
through the bronchoscope. Total RNA was extracted, cDNA libraries
were constructed, and Semiquantitative RT-PCR was performed using
GAPDH gene as internal control. Our result indicated that level
of TGF- β 1and TGF- β 3 mRNA was significantly higher in chemical
gas injured patients than non-injured group (p